Abstract
THE MAIN RESULTS PRESENTED IN THIS ARTICLE MAY BE SUMMARIZED AS FOLLOWS: 1. A test method with deseeded Avena seedlings for small concentrations of auxin and precursors of auxin has been described. 2. This method makes possible quantitative determinations of about ten times as low concentrations of hormone as can be obtained with the standard method, (a) Through an increase in the time of the test, so that nearly all the hormone applied can be utilized. (b) Through an increase in sensitivity of deseeded plants to unilaterally applied small concentrations of hormone. 3. The effect of deseeding in relation to curvature growth is primarily the prevention of auxin regeneration through the removal of the material for auxin synthesis, and in addition the prevention of physiological aging. 4. The mechanism of auxin synthesis in the tip of the coleoptile and the mechanism of auxin regeneration in the new physiological tip have been shown to be identical. 5. The application of the deseeded method is illustrated by determinations of auxin in primary leaves and coleoptile sections of Avena seedlings. 6. The deseeded method has been used as a test method for precursors of auxin obtainable from the coleoptile and from other sources. The method further makes possible a distinction between auxins and these substances which may become activated by the plant. 7. Evidence for the existence of a precursor of auxin in the plant is given (a) indirectly by determinations of the decrease in auxin synthesis in deseeded plants. (b) Directly by its isolation from the plant. 8. Precursors of hetero-auxin are demonstrated; their chemical nature and activation are briefly considered.
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Claims 11
Claim · model-written summary
The deseeded Avena method quantifies about ten times lower hormone concentrations than the standard method.
AvenaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method makes possible quantitative determinations of about ten times as low concentrations of hormone as can be obtained with the standard method”
t0“AVENA”
Claim · model-written summary
Longer assay time allows nearly all applied hormone to be utilized.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through an increase in the time of the test, so that nearly all the hormone applied can be utilized”
t0“AVENA”
Claim · model-written summary
Deseeded plants have increased sensitivity to unilaterally applied low hormone concentrations.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in sensitivity of deseeded plants to unilaterally applied small concentrations of hormone”
t0“AVENA”
Claim · model-written summary
Deseeding prevents auxin regeneration by removing material for auxin synthesis.
- Outcome
- auxin regeneratione0
- Direction
- preventione0
- Qualifier
- removal of the material for auxin synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prevention of auxin regeneration through the removal of the material for auxin synthesis”
t0“AVENA”
Claim · model-written summary
Deseeding prevents physiological aging.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prevention of physiological aging”
e1“The effect of deseeding in relation to curvature growth”
t0“AVENA”
Claim · model-written summary
Auxin synthesis in the coleoptile tip and regeneration in the new physiological tip have identical mechanisms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mechanism of auxin synthesis in the tip of the coleoptile and the mechanism of auxin regeneration in the new physiological tip have been shown to be identical”
t0“AVENA”
Claim · model-written summary
The deseeded method measured auxin in Avena primary leaves and coleoptile sections.
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of the deseeded method is illustrated by determinations of auxin in primary leaves and coleoptile sections of Avena seedlings”
Claim · model-written summary
The deseeded method distinguishes auxins from substances activated by plants.
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The method further makes possible a distinction between auxins and these substances which may become activated by the plant”
t0“AVENA”
Claim · model-written summary
Reduced auxin synthesis in deseeded plants indirectly supports an auxin precursor.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Evidence for the existence of a precursor of auxin in the plant is given (a) indirectly by determinations of the decrease in auxin synthesis in deseeded plants”
t0“AVENA”
Claim · model-written summary
An auxin precursor was isolated from the plant.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Directly by its isolation from the plant”
e1“Evidence for the existence of a precursor of auxin in the plant”
t0“AVENA”
Claim · model-written summary
Precursors of hetero-auxin were demonstrated.
- Outcome
- Precursors of hetero-auxine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Precursors of hetero-auxin are demonstrated”
t0“AVENA”