Abstract
The objectives of this study were to examine antioxidant enzyme responses to drought stress and rewatering at both enzymatic activity and transcript levels and to determine the major antioxidant processes associated with drought tolerance and post-drought recovery for a perennial grass species, kentucky bluegrass ( Poa pratensis ). Antioxidant enzyme responses to drought and rewatering in a drought-tolerant cultivar (Midnight) and a drought-sensitive cultivar (Brilliant) were compared in a growth chamber. Plants were exposed to 22 days of drought stress for ‘Midnight’ and 18 days for ‘Brilliant’ before rewatering to allow the leaf relative water content (RWC) of both cultivars to drop to the same level. ‘Midnight’ exhibited higher photochemical efficiency (F v /F m ) and lower electrolyte leakage compared with ‘Brilliant’ when at the same water deficit status (26% to 28% RWC). After 6 days of rewatering, all physiological parameters returned to the control level for ‘Midnight’, but only F v /F m fully recovered for ‘Brilliant’. The transcript level of cytosolic copper/zinc superoxide dismutase (cyt Cu/Zn SOD) and ascorbate peroxidase (APX) was significantly higher in ‘Midnight’ than in ‘Brilliant’ when exposed to the same level of water deficit (26% to 28% RWC), suggesting that SOD and APX could be involved in scavenging oxidative stress-induced reactive oxygen species in kentucky bluegrass through changes in the level of gene expression. Significantly higher activities of APX, monodehydroascorbate reductase, glutathione reductase, and dehydroascorbate reductase as well as lower lipid peroxidation levels were observed in ‘Midnight’ versus ‘Brilliant’ when exposed to drought. However, the activities of SOD, catalase (CAT), and guaiacol peroxidase (POD) did not differ between the two cultivars. After 6 days of rewatering, ‘Midnight’ displayed significantly higher activity levels of CAT, POD, and APX compared with ‘Brilliant’. The enzyme activity results indicate that enzymes involved in the ascorbate–glutathine cycle may play important roles in antioxidant protection to drought damage, whereas CAT, POD, and APX could be associated with better post-drought recovery in kentucky bluegrass.
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Claims 10
Claim · model-written summary
Midnight had higher photochemical efficiency (Fv/Fm) than Brilliant at the same water deficit (26% to 28% RWC).
- Taxon
- kentucky bluegrass
.jpg?width=60)
Poa pratensise2 - Material
- Midnighte1
- Treatment
- water deficit status (26% to 28% RWC)e1
- Outcome
- photochemical efficiency (F v /F m )e1
- Direction
- highere1
- Comparator
- Brilliante1
- Setting
- growth chambere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“‘Midnight’ exhibited higher photochemical efficiency (F v /F m ) and lower electrolyte leakage compared with ‘Brilliant’ when at the same water deficit status (26% to 28% RWC)”
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e3“were compared in a growth chamber”
Claim · model-written summary
Midnight had lower electrolyte leakage than Brilliant at the same water deficit (26% to 28% RWC).
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte1
- Treatment
- water deficit status (26% to 28% RWC)e1
- Outcome
- electrolyte leakagee1
- Direction
- lowere1
- Comparator
- Brilliante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“‘Midnight’ exhibited higher photochemical efficiency (F v /F m ) and lower electrolyte leakage compared with ‘Brilliant’ when at the same water deficit status (26% to 28% RWC)”
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
Claim · model-written summary
After 6 days of rewatering, all physiological parameters of Midnight returned to the control level.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte4
- Treatment
- rewateringe4
- Outcome
- physiological parameterse4
- Direction
- returnede4
- Comparator
- control levele4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e4“After 6 days of rewatering, all physiological parameters returned to the control level for ‘Midnight’, but only F v /F m fully recovered for ‘Brilliant’”
Claim · model-written summary
After 6 days of rewatering, only Fv/Fm fully recovered in Brilliant.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Brilliante4
- Treatment
- rewateringe4
- Outcome
- F v /F me4
- Direction
- fully recoverede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e4“After 6 days of rewatering, all physiological parameters returned to the control level for ‘Midnight’, but only F v /F m fully recovered for ‘Brilliant’”
Claim · model-written summary
Transcript levels of cyt Cu/Zn SOD and APX were significantly higher in Midnight than in Brilliant at the same water deficit (26% to 28% RWC).
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte5
- Treatment
- water deficit (26% to 28% RWC)e5
- Gene
- cytosolic copper/zinc superoxide dismutase (cyt Cu/Zn SOD) and ascorbate peroxidase (APX)e5
- Outcome
- transcript levele5
- Direction
- highere5
- Comparator
- Brilliante5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e5“The transcript level of cytosolic copper/zinc superoxide dismutase (cyt Cu/Zn SOD) and ascorbate peroxidase (APX) was significantly higher in ‘Midnight’ than in ‘Brilliant’ when exposed to the same level of water deficit (26% to 28% RWC)”
Claim · model-written summary
Under drought, Midnight had higher activities of APX, monodehydroascorbate reductase and glutathione reductase than Brilliant.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte6
- Treatment
- droughte6
- Outcome
- activities of APX, monodehydroascorbate reductase, glutathione reductasee6
- Direction
- highere6
- Comparator
- Brilliante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e6“Significantly higher activities of APX, monodehydroascorbate reductase, glutathione reductase, and dehydroascorbate reductase as well as lower lipid peroxidation levels were observed in ‘Midnight’ versus ‘Brilliant’ when exposed to drought”
Claim · model-written summary
Under drought, Midnight had higher dehydroascorbate reductase activity than Brilliant.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte6
- Treatment
- droughte6
- Outcome
- dehydroascorbate reductasee6
- Direction
- highere6
- Comparator
- Brilliante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e6“Significantly higher activities of APX, monodehydroascorbate reductase, glutathione reductase, and dehydroascorbate reductase as well as lower lipid peroxidation levels were observed in ‘Midnight’ versus ‘Brilliant’ when exposed to drought”
Claim · model-written summary
Under drought, Midnight had lower lipid peroxidation levels than Brilliant.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte6
- Treatment
- droughte6
- Outcome
- lipid peroxidation levelse6
- Direction
- lowere6
- Comparator
- Brilliante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e6“Significantly higher activities of APX, monodehydroascorbate reductase, glutathione reductase, and dehydroascorbate reductase as well as lower lipid peroxidation levels were observed in ‘Midnight’ versus ‘Brilliant’ when exposed to drought”
Claim · model-written summary
SOD, CAT and POD activities did not differ between the two cultivars.
- Taxon
- kentucky bluegrassPoa pratensise2
- Outcome
- activities of SOD, catalase (CAT), and guaiacol peroxidase (POD)e7
- Direction
- did not differe7
- Comparator
- the two cultivarse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e7“However, the activities of SOD, catalase (CAT), and guaiacol peroxidase (POD) did not differ between the two cultivars”
Claim · model-written summary
After 6 days of rewatering, Midnight had significantly higher CAT, POD and APX activity than Brilliant.
- Taxon
- kentucky bluegrassPoa pratensise2
- Material
- Midnighte3
- Treatment
- rewateringe3
- Outcome
- activity levels of CAT, POD, and APXe3
- Direction
- highere3
- Comparator
- Brilliante3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“perennial grass species, kentucky bluegrass ( Poa pratensis )”
e3“After 6 days of rewatering, ‘Midnight’ displayed significantly higher activity levels of CAT, POD, and APX compared with ‘Brilliant’”
The same questions in other papers
drought · physiology: photosynthesis · Poa pratensis (Kentucky bluegrass)Positive and negative results
1 paper in the same direction as this paper · 6 in the opposite direction
- negative
- positive
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.