Abstract
Using the method described by GRIGA (1998), embryoids were obtained (through direct or indirect somatic embryogenesis) in cultures of shoot apical meristems of the following pea cultivars: Bankier, Dino, Hazard, Ilowiecki, Konserwowy IHAR, Kosynier, Makler, Oskar, Pegaz, as well as unregistered line HM-6. With cultivars Izolda and Lantra the efforts at somatic embryogenesis (SE) induction remained unsuccessful. The highest responsiveness to SE induction was observed (after 14 days of treatment with a relatively low concentration of picloram – 2.5 μM) in cultivars Oskar, Hazard and line HM-6, in which embryoids were formed with frequencies of 31, 15.9 and 12.5%, respectively. Increasing picloram level to 5 μM and extending period of induction to 28 days, it was possible to obtain SE efficiency above 10% in cultivars Konserwowy IHAR, Dino and Kosynier. Photoperiod affected SE efficiency and the degree and direction of this influence greatly depended on pea cultivar.
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Claims 7
Claim · model-written summary
Embryoids were obtained in nine named cultivars and line HM-6.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- shoot apical meristemse0
- Outcome
- embryoids were obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“embryoids were obtained (through direct or indirect somatic embryogenesis) in cultures of shoot apical meristems of the following pea cultivars: Bankier, Dino, Hazard, Ilowiecki, Konserwowy IHAR, Kosynier, Makler, Oskar, Pegaz, as well as unregistered line HM-6”
Claim · model-written summary
Somatic embryogenesis induction was unsuccessful in Izolda and Lantra.
- Taxon
- peaLathyrus oleraceust0
- Material
- Izolda and Lantrae0
- Outcome
- somatic embryogenesis (SE) induction remained unsuccessfule0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With cultivars Izolda and Lantra the efforts at somatic embryogenesis (SE) induction remained unsuccessful”
t0“Capacity for Somatic Embryogenesis in Different Pea Cultivars”
Claim · model-written summary
Oskar formed embryoids at 31% after the stated picloram treatment.
- Taxon
- peaLathyrus oleraceust0
- Material
- Oskare0
- Treatment
- picloram – 2.5 μMe0
- Outcome
- embryoids were formed with frequenciese0
- Value
- 31e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after 14 days of treatment with a relatively low concentration of picloram – 2.5 μM) in cultivars Oskar, Hazard and line HM-6, in which embryoids were formed with frequencies of 31, 15.9 and 12.5%, respectively”
t0“Capacity for Somatic Embryogenesis in Different Pea Cultivars”
Claim · model-written summary
Hazard formed embryoids at 15.9% after the stated picloram treatment.
- Taxon
- peaLathyrus oleraceust0
- Material
- Hazarde0
- Treatment
- picloram – 2.5 μMe0
- Outcome
- embryoids were formed with frequenciese0
- Value
- 15.9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after 14 days of treatment with a relatively low concentration of picloram – 2.5 μM) in cultivars Oskar, Hazard and line HM-6, in which embryoids were formed with frequencies of 31, 15.9 and 12.5%, respectively”
t0“Capacity for Somatic Embryogenesis in Different Pea Cultivars”
Claim · model-written summary
HM-6 formed embryoids at 12.5% after the stated picloram treatment.
- Taxon
- peaLathyrus oleraceust0
- Material
- HM-6e0
- Treatment
- picloram – 2.5 μMe0
- Outcome
- embryoids were formed with frequenciese0
- Value
- 12.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after 14 days of treatment with a relatively low concentration of picloram – 2.5 μM) in cultivars Oskar, Hazard and line HM-6, in which embryoids were formed with frequencies of 31, 15.9 and 12.5%, respectively”
t0“Capacity for Somatic Embryogenesis in Different Pea Cultivars”
Claim · model-written summary
At 5 μM picloram for 28 days, Konserwowy IHAR, Dino and Kosynier exceeded 10% embryogenesis efficiency.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- picloram level to 5 μMe0
- Outcome
- SE efficiencye0
- Value
- above 10%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing picloram level to 5 μM and extending period of induction to 28 days, it was possible to obtain SE efficiency above 10% in cultivars Konserwowy IHAR, Dino and Kosynier”
t0“Capacity for Somatic Embryogenesis in Different Pea Cultivars”
Claim · model-written summary
Photoperiod effects on somatic embryogenesis efficiency depended on cultivar.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- Photoperiode0
- Outcome
- SE efficiencye0
- Qualifier
- greatly depended on pea cultivare0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photoperiod affected SE efficiency and the degree and direction of this influence greatly depended on pea cultivar”