Abstract
Cauliflower (Brassica oleracea var. botrytis) was transformed, using Agrobacterium tumefaciens, with an autoregulated isopentenyl transferase (ipt) gene under the control of a senescence‐associated gene promoter, pSAG12, isolated from Arabidopsis thaliana. The effect of introducing this chimeric construct on cytokinin (CK) content, chlorophyll retention, and plant morphology and development were investigated. A range of CK and chlorophyll contents was found among the individual primary transformants. Progeny were studied from one of the primary transformed lines that did not have elevated cytokinin content and was phenotypically similar to the parent line but displayed delayed leaf senescence. The pSAG12:ipt gene was inherited in a Mendelian manner, and the effect of this gene on senescence‐related parameters was observed in a number of the progeny. While the pSAG12:ipt progeny did exhibit delayed leaf senescence, it was accompanied by undesirable agronomic traits, including less synchronous curd initiation, smaller curd size, and greater susceptibility to fungal infection.
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Claims 10
Claim · model-written summary
Primary cauliflower transformants varied in cytokinin and chlorophyll content.
- Taxon
- Cauliflower
Brassica oleracea var. botrytise0 - Material
- individual primary transformantse1
- Outcome
- CK and chlorophyll contentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“A range of CK and chlorophyll contents was found among the individual primary transformants”
Claim · model-written summary
The selected primary transformed line had no elevated cytokinin content.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Material
- primary transformed linese1
- Outcome
- cytokinin contente1
- Direction
- did not have elevatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“one of the primary transformed lines that did not have elevated cytokinin content”
Claim · model-written summary
The selected line was phenotypically similar to its parent line.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Outcome
- phenotypically similare1
- Comparator
- parent linee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“was phenotypically similar to the parent line”
Claim · model-written summary
The selected transformed line displayed delayed leaf senescence.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Organ / stage
- leafe1
- Outcome
- leaf senescencee1
- Direction
- delayede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“displayed delayed leaf senescence”
Claim · model-written summary
The pSAG12:ipt gene was inherited in a Mendelian manner.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Gene
- pSAG12:ipt genee1
- Outcome
- inherited in a Mendelian mannere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“The pSAG12:ipt gene was inherited in a Mendelian manner”
Claim · model-written summary
Senescence related effects of the gene were seen in several progeny.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Material
- progenye1
- Outcome
- senescence‐related parameterse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“the effect of this gene on senescence‐related parameters was observed in a number of the progeny”
Claim · model-written summary
pSAG12:ipt progeny exhibited delayed leaf senescence.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Material
- pSAG12:ipt progenye1
- Outcome
- leaf senescencee1
- Direction
- delayede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“the pSAG12:ipt progeny did exhibit delayed leaf senescence”
Claim · model-written summary
pSAG12:ipt progeny had less synchronous curd initiation.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Outcome
- curd initiatione1
- Direction
- less synchronouse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“less synchronous curd initiation”
Claim · model-written summary
pSAG12:ipt progeny had smaller curds.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Outcome
- curd sizee1
- Direction
- smallere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“smaller curd size”
Claim · model-written summary
pSAG12:ipt progeny had greater susceptibility to fungal infection.
- Taxon
- CauliflowerBrassica oleracea var. botrytise0
- Outcome
- susceptibility to fungal infectione1
- Direction
- greatere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cauliflower (Brassica oleracea var. botrytis)”
e1“greater susceptibility to fungal infection”