Paper · 2009 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Effect of sorbitol on callus induction and plant regeneration in wheat.

Mahmood Ul Hassan; Zaheer Ahmed; Muhammad Munir; et al.

11
claims
22
verbatim quotes
20
citations
1
research areas
✓
Open access

Abstract

Six wheat genotypes were evaluated for their response to callus induction and regeneration on MS medium modified with different concentrations of sorbitol, that is, 0, 10, 20, 30 gL-1 along with optimum (3 mgL-1) concentration of 2,4-D. Variability was observed among different genotypes for callus induction. Highest callus induction frequency was shown by Wafaq- 2001, which was about 85.62% followed by Inqalab-91 which showed 71.94% callus induction. While minimum callus induction frequency was shown by Saleem-2000 which was about 51.21%. Regarding sorbitol concentration highest average callus induction frequency (79.20%) was obtained at 20 gL-1 and lowest average callus induction frequency (59.20%) was observed at 30 gL-1. In Wafaq-2001 and Inqalab-91 plant regeneration increased gradually by increasing the sorbitol concentration from 0 to 20 gL-1 but then it decreased. Similarly Auqab-2002 had no regeneration al all on non-sorbitol medium but showed regeneration on addition of sorbitol. Similarly time duration required for plant regeneration also decreased by increasing the concentration of sorbitol. It was also observed that sorbitol has given more strength to regenerated plant.

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

Callus induction varied among wheat genotypes.

Material
different genotypese1
Outcome
callus inductione1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e1

“Variability was observed among different genotypes for callus induction”

✓ located verbatim in the source · abstract, characters 237–308 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Wafaq-2001 had the highest callus induction frequency, about 85.62%.

Material
Wafaq- 2001e2
Outcome
callus induction frequencye2
Direction
Higheste2
Value
85.62%e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e2

“Highest callus induction frequency was shown by Wafaq- 2001, which was about 85.62%”

✓ located verbatim in the source · abstract, characters 310–393 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Inqalab-91 showed a callus induction frequency of 71.94%.

Material
Inqalab-91e3
Outcome
callus inductione3
Value
71.94%e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e3

“followed by Inqalab-91 which showed 71.94% callus induction”

✓ located verbatim in the source · abstract, characters 394–453 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Saleem-2000 had the minimum callus induction frequency, about 51.21%.

Material
Saleem-2000e4
Outcome
callus induction frequencye4
Direction
minimume4
Value
51.21%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e4

“While minimum callus induction frequency was shown by Saleem-2000 which was about 51.21%”

✓ located verbatim in the source · abstract, characters 455–543 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Sorbitol at 20 gL-1 gave the highest average callus induction frequency, 79.20%.

Treatment
20 gL-1e5
Outcome
average callus induction frequencye5
Direction
higheste5
Value
79.20%e5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e5

“Regarding sorbitol concentration highest average callus induction frequency (79.20%) was obtained at 20 gL-1”

✓ located verbatim in the source · abstract, characters 545–653 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Sorbitol at 30 gL-1 gave the lowest average callus induction frequency, 59.20%.

Treatment
30 gL-1e6
Outcome
average callus induction frequencye6
Direction
loweste6
Value
59.20%e6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e6

“lowest average callus induction frequency (59.20%) was observed at 30 gL-1”

✓ located verbatim in the source · abstract, characters 658–732 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Plant regeneration in Wafaq-2001 and Inqalab-91 increased gradually as sorbitol rose from 0 to 20 gL-1.

Material
Wafaq-2001 and Inqalab-91e7
Treatment
sorbitol concentration from 0 to 20 gL-1e7
Outcome
plant regeneratione7
Direction
increased graduallye7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e7

“In Wafaq-2001 and Inqalab-91 plant regeneration increased gradually by increasing the sorbitol concentration from 0 to 20 gL-1 but then it decreased”

✓ located verbatim in the source · abstract, characters 734–882 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Plant regeneration in Wafaq-2001 and Inqalab-91 decreased beyond 20 gL-1 sorbitol.

Material
Wafaq-2001 and Inqalab-91e7
Treatment
20 gL-1e7
Outcome
plant regeneratione7
Direction
decreasede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e7

“In Wafaq-2001 and Inqalab-91 plant regeneration increased gradually by increasing the sorbitol concentration from 0 to 20 gL-1 but then it decreased”

✓ located verbatim in the source · abstract, characters 734–882 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Auqab-2002 regenerated only when sorbitol was added to the medium.

Material
Auqab-2002e1
Treatment
addition of sorbitole1
Outcome
regeneratione1
Comparator
non-sorbitol mediume1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e1

“Similarly Auqab-2002 had no regeneration al all on non-sorbitol medium but showed regeneration on addition of sorbitol”

✓ located verbatim in the source · abstract, characters 884–1002 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher sorbitol concentration shortened the time required for plant regeneration.

Treatment
increasing the concentration of sorbitole1
Outcome
time duration required for plant regeneratione1
Direction
decreasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e1

“Similarly time duration required for plant regeneration also decreased by increasing the concentration of sorbitol”

✓ located verbatim in the source · abstract, characters 1004–1118 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sorbitol gave regenerated wheat plants more strength.

Treatment
sorbitole1
Outcome
strength to regenerated plante1
Direction
moree1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six wheat genotypes were evaluated”

✓ located verbatim in the source · abstract, characters 0–34 · show in text ↑
e1

“It was also observed that sorbitol has given more strength to regenerated plant”

✓ located verbatim in the source · abstract, characters 1120–1199 · show in text ↑

glucitol · growth · Triticum aestivum (wheat)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.