Abstract
Scutellar somatic embryos are most commonly used as a target tissue for transformation and regeneration of transgenic wheat plants. Tissue culture responsiveness of elite varieties of wheat is one of the critical factors limiting high-frequency transformation. This study investigated the influence of sorbitol and sucroseinduced osmotic stress on growth and plantlet regeneration in callus cultures of wheat (Triticum aestivum L. em Thell). Immature embryos, at early-medium milking stages, of field grown wheat cvs., HUW206, HUW234, Sonalika and HD2009, were induced to form the callus on MS medium supplemented with 2, 4-D and 3% sucrose alone/or in a combination of sucrose and sorbitol. Fresh weight of callus was significantly promoted when 1.5% (w/v) sorbitol was added to the MS medium in conjunction with 3% (w/v) sucrose. However, higher concentration of sorbitol concentration (3% w/v) in conjunction with 3% (w/v) sucrose did not further improve fresh weight. Sorbitol in combination with sucrose caused a decrease in the water content from calli and promoted embryogenic callus formation. After 4 weeks, a high frequency (10–25%) shoot formation was obtained on MS media, IAA 1 mgL1 and zeatin 1 mgL−1 in supplementation with sucrose and sorbitol. However, optimum plantlet regeneration (45–65%) was found on subsequent subculture on regeneration media containing 3% (w/v) sucrose and 3% (w/v) sorbitol. Osmotic stress induced by the combination of sucrose and sorbitol is beneficial for embryogenecity of the friable calli and high-frequency plantlet regeneration.
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Claims 6
Claim · model-written summary
Adding 1.5% sorbitol to 3% sucrose significantly increased callus fresh weight.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- calluse0
- Treatment
- 1.5% (w/v) sorbitole0
- Outcome
- Fresh weight of calluse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fresh weight of callus was significantly promoted when 1.5% (w/v) sorbitol was added to the MS medium in conjunction with 3% (w/v) sucrose.”
t0“Wheat”
Claim · model-written summary
Three percent sorbitol with 3% sucrose did not further improve callus fresh weight.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sorbitol concentration (3% w/v) in conjunction with 3% (w/v) sucrosee0
- Outcome
- fresh weighte0
- Direction
- did not further improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher concentration of sorbitol concentration (3% w/v) in conjunction with 3% (w/v) sucrose did not further improve fresh weight.”
t0“Wheat”
Claim · model-written summary
Sorbitol with sucrose decreased callus water content.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Sorbitol in combination with sucrosee0
- Outcome
- water content from callie0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorbitol in combination with sucrose caused a decrease in the water content from calli”
t0“Wheat”
Claim · model-written summary
Sorbitol with sucrose promoted embryogenic callus formation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Sorbitol in combination with sucrosee0
- Outcome
- embryogenic callus formatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorbitol in combination with sucrose caused a decrease in the water content from calli and promoted embryogenic callus formation.”
t0“Wheat”
Claim · model-written summary
After four weeks, supplemented medium yielded 10–25% shoot formation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrose and sorbitole0
- Outcome
- shoot formatione0
- Value
- 10–25%e0
- Qualifier
- After 4 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 4 weeks, a high frequency (10–25%) shoot formation was obtained on MS media, IAA 1 mgL1 and zeatin 1 mgL−1 in supplementation with sucrose and sorbitol.”
t0“Wheat”
Claim · model-written summary
Subculture with 3% sucrose and 3% sorbitol gave optimum regeneration of 45–65%.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- 3% (w/v) sucrose and 3% (w/v) sorbitole0
- Outcome
- plantlet regeneratione0
- Direction
- optimume0
- Value
- 45–65%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimum plantlet regeneration (45–65%) was found on subsequent subculture on regeneration media containing 3% (w/v) sucrose and 3% (w/v) sorbitol.”
t0“Wheat”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.