Claim · model-written summary
Subculture with 3% sucrose and 3% sorbitol gave optimum regeneration of 45–65%.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- 3% (w/v) sucrose and 3% (w/v) sorbitole0
- Outcome
- plantlet regeneratione0
- Direction
- optimume0
- Value
- 45–65%e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimum plantlet regeneration (45–65%) was found on subsequent subculture on regeneration media containing 3% (w/v) sucrose and 3% (w/v) sorbitol.”
t0“Wheat”
Title
Sorbitol and Sucrose-Induced Osmotic Stress on Growth of Wheat Callus and Plantlet Regeneration
Vinay Kumar Mishra; Ram Singh Current Trends in Biotechnology and Pharmacy 2016
Other claims from this paper (5)
- Intervention effect Adding 1.5% sorbitol to 3% sucrose significantly increased callus fresh weight.
- Intervention effect Three percent sorbitol with 3% sucrose did not further improve callus fresh weight.
- Intervention effect Sorbitol with sucrose decreased callus water content.
- Intervention effect Sorbitol with sucrose promoted embryogenic callus formation.
- Observation After four weeks, supplemented medium yielded 10–25% shoot formation.