Abstract
The research work was carried out to study the effect of sucrose induced osmotic stress on callus growth and biochemical aspects of two wheat genotypes (S-24 and MH-97). The seeds were cultured on Linsmaier and Skoog medium containing 30g sucrose, 8g agar, 5mg L -1 thiamine HCl and 3mg L -1 2, 4-Dichlorophenoxyacetic acid. One month old calli were subcultured for 15 d in liquid LS-medium supplemented with same concentration of thiamine HCl and 2,4-D. and different concentrations of sucrose [control, (3%), 4%, 6% 5% and 8%]. After 15 d of sucrose induced osmotic stress the results revealed that relative growth rate (fresh), macro cations (K + , Ca 2+ , Mg 2+ )and micro cations (Mn 2+ , Fe 2+ ) significantly decreased, while dry weight, free proline, total soluble carbohydrates contents and water relation parameters significantly increased (more negative) as concentration of sucrose increased in the culture medium. The effects of sucrose induced osmotic stress was greater on MH-97 than S-24.It is concluded that increasing sucrose concentrations in the medium above control caused osmotic stress and it also been found that accumulation of free proline and total soluble carbohydrates accumulated in greater amount responsible for turgor maintenance and increase in callus dry weights.
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Claims 8
Claim · model-written summary
Increasing sucrose significantly reduced fresh relative growth rate of wheat calli.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- sucrosee1
- Outcome
- relative growth rate (fresh)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative growth rate (fresh), macro cations (K + , Ca 2+ , Mg 2+ )and micro cations (Mn 2+ , Fe 2+ ) significantly decreased”
e1“as concentration of sucrose increased in the culture medium”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Increasing sucrose significantly reduced K, Ca, Mg, Mn and Fe cations in wheat calli.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrosee1
- Outcome
- macro cations (K + , Ca 2+ , Mg 2+ )and micro cations (Mn 2+ , Fe 2+ )e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“macro cations (K + , Ca 2+ , Mg 2+ )and micro cations (Mn 2+ , Fe 2+ ) significantly decreased”
e1“as concentration of sucrose increased in the culture medium”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Increasing sucrose significantly increased wheat callus dry weight.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrosee0
- Outcome
- dry weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry weight, free proline, total soluble carbohydrates contents and water relation parameters significantly increased (more negative) as concentration of sucrose increased in the culture medium.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Increasing sucrose significantly increased free proline in wheat calli.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrosee0
- Outcome
- free prolinee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry weight, free proline, total soluble carbohydrates contents and water relation parameters significantly increased (more negative) as concentration of sucrose increased in the culture medium.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Increasing sucrose significantly increased total soluble carbohydrate content of wheat calli.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrosee0
- Outcome
- total soluble carbohydrates contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry weight, free proline, total soluble carbohydrates contents and water relation parameters significantly increased (more negative) as concentration of sucrose increased in the culture medium.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Water relation parameters in wheat calli became more negative as sucrose increased.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sucrosee0
- Outcome
- water relation parameterse0
- Direction
- more negativee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“water relation parameters significantly increased (more negative) as concentration of sucrose increased in the culture medium.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
Sucrose osmotic stress affected wheat genotype MH-97 more than S-24.
- Taxon
- wheatTriticum aestivumt0
- Material
- MH-97e0
- Outcome
- effects of sucrose induced osmotic stresse0
- Direction
- greatere0
- Comparator
- S-24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effects of sucrose induced osmotic stress was greater on MH-97 than S-24.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
Claim · model-written summary
The authors attributed turgor maintenance and increased callus dry weight to accumulated proline and carbohydrates.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- turgor maintenance and increase in callus dry weightse0
- Qualifier
- accumulation of free proline and total soluble carbohydratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of free proline and total soluble carbohydrates accumulated in greater amount responsible for turgor maintenance and increase in callus dry weights.”
t0“EFFECT OF SUCROSE INDUCED OSMOTIC STRESS ON CALLUS GROWTH AND BIOCHEMICAL ASPECTS OF TWO WHEAT GENOTYPES”
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8 papers in the same direction as this paper · 7 in the opposite direction
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.