Paper · 2002 · Acta Agriculturae Boreall—sinica

Effects of Carbohydrate Composition and Concentration on Anther Culture and Isolated Microspores Culture in Wheat( Triticum aestivum L.)

Lan Su

13
claims
26
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

The carbohydrate composition containing maltose,sucrose and the mixture of maltose and sucrose respectively in induction medium of wheat anther culture were studied.The callus rate induced in medium using maltose as the sole carbohydrate was found to be the highest,followed by that using the mixture of maltose and sucrose,and the callus rate in that using sucrose medium was the lowest.In the whole carbohydrate experiment,the callus rate increased with the increasing of maltose content.The effects of the carbohydrate on callus rate related to their hybolysis products.Maltose was the best carbohydrate in isolated microspores culture.The microspores incubated in other carbohydrate did not form callus.Some of microspores incubated for 3 days in only maltose medium had viability.It was concluded that high concentration of sucrose,glucose and fructose was toxic to the microspores.The initial concentration of glucose up to 2 0 mmol/L resulted in the microspores death.When high concentration of glucose was added after 1 day incubation with moltose,the microspores was still viable.The viability of microspore increased with the incubation time.

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Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maltose gave the highest anther culture callus rate.

Treatment
maltose as the sole carbohydratee0
Outcome
callus ratee0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“callus rate induced in medium using maltose as the sole carbohydrate was found to be the highest”

✓ located verbatim in the source · abstract, characters 169–265 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The maltose and sucrose mixture gave an intermediate anther culture callus rate.

Treatment
mixture of maltose and sucrosee0
Outcome
callus ratee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“callus rate induced in medium using maltose as the sole carbohydrate was found to be the highest,followed by that using the mixture of maltose and sucrose”

✓ located verbatim in the source · abstract, characters 169–323 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Sucrose medium gave the lowest anther culture callus rate.

Treatment
sucrose mediume0
Outcome
callus ratee0
Direction
loweste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“callus rate in that using sucrose medium was the lowest”

✓ located verbatim in the source · abstract, characters 332–387 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus rate increased with maltose content.

Treatment
maltose contente0
Outcome
callus ratee0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“callus rate increased with the increasing of maltose content”

✓ located verbatim in the source · abstract, characters 429–489 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maltose was the best carbohydrate for isolated microspore culture.

Treatment
Maltosee0
Outcome
best carbohydrate in isolated microspores culturee0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“Maltose was the best carbohydrate in isolated microspores culture”

✓ located verbatim in the source · abstract, characters 573–638 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Microspores in other carbohydrates did not form callus.

Treatment
other carbohydratee0
Outcome
did not form calluse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“microspores incubated in other carbohydrate did not form callus”

✓ located verbatim in the source · abstract, characters 643–706 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Some microspores remained viable after three days in maltose-only medium.

Treatment
only maltose mediume0
Outcome
had viabilitye0
Qualifier
for 3 dayse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“Some of microspores incubated for 3 days in only maltose medium had viability”

✓ located verbatim in the source · abstract, characters 707–784 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High sucrose concentration was toxic to microspores.

Treatment
high concentration of sucrosee0
Outcome
toxic to the microsporese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“high concentration of sucrose,glucose and fructose was toxic to the microspores”

✓ located verbatim in the source · abstract, characters 807–886 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High glucose concentration was toxic to microspores.

Treatment
glucosee0
Outcome
toxic to the microsporese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“high concentration of sucrose,glucose and fructose was toxic to the microspores”

✓ located verbatim in the source · abstract, characters 807–886 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High fructose concentration was toxic to microspores.

Treatment
fructosee0
Outcome
toxic to the microsporese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“high concentration of sucrose,glucose and fructose was toxic to the microspores”

✓ located verbatim in the source · abstract, characters 807–886 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Initial glucose at 2 0 mmol/L killed microspores.

Treatment
glucose up to 2 0 mmol/Le0
Outcome
microspores deathe0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“initial concentration of glucose up to 2 0 mmol/L resulted in the microspores death”

✓ located verbatim in the source · abstract, characters 891–974 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Microspores remained viable when high glucose was added after one day with maltose.

Treatment
high concentration of glucosee0
Outcome
microspores was still viablee0
Qualifier
after 1 day incubation with moltosee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“When high concentration of glucose was added after 1 day incubation with moltose,the microspores was still viable”

✓ located verbatim in the source · abstract, characters 975–1088 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Microspore viability increased with incubation time.

Treatment
incubation timee0
Outcome
viability of microsporee0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat”

✓ located verbatim in the source · title, characters 108–113 · show in text ↑
e0

“viability of microspore increased with the incubation time”

✓ located verbatim in the source · abstract, characters 1093–1151 · show in text ↑

glucose · survival · Triticum aestivum (wheat)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

sucrose · growth · Triticum aestivum (wheat)Positive and negative results

16 papers: 9 positive, 7 negative or no effect

6 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

carbohydrate · growth · Triticum aestivum (wheat)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

maltose · growth · Triticum aestivum (wheat)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

in vitro · growth · Triticum aestivum (wheat)Positive and negative results

106 papers: 73 positive, 33 negative or no effect

72 papers in the same direction as this paper · 31 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

102 papers more in this group

See all 106 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.