Abstract
Brassinosteroids (BRs), a group of naturally occurring plant steroidal compounds, are essential for plant growth, development and stress tolerance. Recent studies showed that BRs could induce systemic tolerance to biotic and abiotic stresses; however, the molecular mechanisms by which BRs signals lead to responses in the whole plant are largely unknown. In this study, 24-epibrassinosteroid (EBR)-induced systemic tolerance in Cucumis sativus L. cv. Jinyan No. 4 was analyzed through the assessment of symptoms of photooxidative stress by chlorophyll fluorescence imaging pulse amplitude modulation. Expression of defense/stress related genes were induced in both treated local leaves and untreated systemic leaves by local EBR application. With the suppressive subtractive hybridization (SSH) library using cDNA from the phloem sap of EBR-treated plants as the tester and distilled water (DW)-treated plants as the driver, 14 transcripts out of 260 clones were identified. Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR) validated the specific up-regulation of these transcripts. Of the differentially expressed transcripts with known functions, transcripts for the selected four cDNAs, which encode an auxin-responsive protein (IAA14), a putative ankyrin-repeat protein, an F-box protein (PP2), and a major latex, pathogenesis-related (MLP)-like protein, were induced in local leaves, systemic leaves and roots after foliar application of EBR onto mature leaves. Our results demonstrated that EBR-induced systemic tolerance is accompanied with increased transcript of genes in the defense response in other organs. The potential role of phloem mRNAs as signaling components in mediating BR-regulated systemic resistance is discussed.
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Claims 6
Claim · model-written summary
Local EBR application induced defense and stress gene expression in treated leaves.
- Taxon
- Cucumis sativus
Cucumis sativust0 - Organ / stage
- treated local leavese0
- Treatment
- local EBR applicatione0
- Outcome
- Expression of defense/stress related genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of defense/stress related genes were induced in both treated local leaves and untreated systemic leaves by local EBR application”
t0“Cucumis sativus”
Claim · model-written summary
Local EBR application induced defense and stress gene expression in untreated systemic leaves.
- Taxon
- Cucumis sativusCucumis sativust0
- Organ / stage
- untreated systemic leavese0
- Treatment
- local EBR applicatione0
- Outcome
- Expression of defense/stress related genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of defense/stress related genes were induced in both treated local leaves and untreated systemic leaves by local EBR application”
t0“Cucumis sativus”
Claim · model-written summary
The phloem sap SSH library identified 14 transcripts among 260 clones.
- Taxon
- Cucumis sativusCucumis sativust0
- Outcome
- transcriptse0
- Value
- 14 transcripts out of 260 clonese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 transcripts out of 260 clones were identified”
t0“Cucumis sativus”
Claim · model-written summary
RT-qPCR validated specific up-regulation of the identified transcripts.
- Taxon
- Cucumis sativusCucumis sativust0
- Outcome
- specific up-regulation of these transcriptse0
- Direction
- up-regulatione0
- Comparator
- RT-qPCRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR) validated the specific up-regulation of these transcripts”
t0“Cucumis sativus”
Claim · model-written summary
EBR foliar application induced transcripts for four selected cDNAs in local leaves, systemic leaves and roots.
- Taxon
- Cucumis sativusCucumis sativust0
- Organ / stage
- local leaves, systemic leaves and rootse0
- Treatment
- foliar application of EBRe0
- Gene
- IAA14e0
- Outcome
- transcripts for the selected four cDNAse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts for the selected four cDNAs, which encode an auxin-responsive protein (IAA14), a putative ankyrin-repeat protein, an F-box protein (PP2), and a major latex, pathogenesis-related (MLP)-like protein, were induced in local leaves, systemic leaves and roots after foliar application of EBR onto mature leaves”
t0“Cucumis sativus”
Claim · model-written summary
EBR-induced systemic tolerance was accompanied by increased defense-response gene transcripts in other organs.
- Taxon
- Cucumis sativusCucumis sativust0
- Organ / stage
- other organse0
- Treatment
- EBRe0
- Outcome
- transcript of genes in the defense responsee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EBR-induced systemic tolerance is accompanied with increased transcript of genes in the defense response in other organs”
t0“Cucumis sativus”
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