Abstract
We had previously shown that several transcription factors of the ethylene (ET) response factor (ERF) family were induced with different but overlapping kinetics following challenge of Arabidopsis (Arabidopsis thaliana) with Pseudomonas syringae pv tomato DC3000 (avrRpt2). One of these genes, a transcriptional activator, AtERF14, was induced at the same time as ERF-target genes (ChiB, basic chitinase). To unravel the potential function of AtERF14 in regulating the plant defense response, we have analyzed gain- and loss-of-function mutants. We show here that AtERF14 has a prominent role in the plant defense response, since overexpression of AtERF14 had dramatic effects on both plant phenotype and defense gene expression and AtERF14 loss-of-function mutants showed impaired induction of defense genes following exogenous ET treatment and increased susceptibility to Fusarium oxysporum. Moreover, the expression of other ERF genes involved in defense and ET/jasmonic acid responses, such as ERF1 and AtERF2, depends on AtERF14 expression. A number of ERFs have been shown to function in the defense response through overexpression. However, the effect of loss of AtERF14 function on defense gene expression, pathogen resistance, and regulation of the expression of other ERF genes is unique thus far. These results suggest a unique role for AtERF14 in regulating the plant defense response.
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Claims 6
Claim · model-written summary
AtERF14 was induced at the same time as ERF target genes after bacterial challenge.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Interactor
- Pseudomonas syringae pv tomato DC3000 (avrRpt2)
Pseudomonas syringaee2pathogen - Gene
- AtERF14e1
- Outcome
- induced at the same time as ERF-target genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“AtERF14, was induced at the same time as ERF-target genes (ChiB, basic chitinase)”
e2“following challenge of Arabidopsis (Arabidopsis thaliana) with Pseudomonas syringae pv tomato DC3000 (avrRpt2)”
Claim · model-written summary
AtERF14 overexpression affected the Arabidopsis plant phenotype.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Treatment
- overexpression of AtERF14e1
- Gene
- AtERF14e1
- Outcome
- plant phenotypee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“overexpression of AtERF14 had dramatic effects on both plant phenotype and defense gene expression”
Claim · model-written summary
AtERF14 overexpression affected defense gene expression.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Treatment
- overexpression of AtERF14e1
- Gene
- AtERF14e1
- Outcome
- defense gene expressione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“overexpression of AtERF14 had dramatic effects on both plant phenotype and defense gene expression”
Claim · model-written summary
Loss of AtERF14 function impaired induction of defense genes after exogenous ethylene.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- AtERF14 loss-of-function mutantse1
- Treatment
- exogenous ET treatmente1
- Gene
- AtERF14e1
- Outcome
- induction of defense genese1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“AtERF14 loss-of-function mutants showed impaired induction of defense genes following exogenous ET treatment”
Claim · model-written summary
AtERF14 loss-of-function mutants were more susceptible to Fusarium oxysporum.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Material
- AtERF14 loss-of-function mutantse1
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume1pathogen - Gene
- AtERF14e1
- Outcome
- susceptibility to Fusarium oxysporume1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“AtERF14 loss-of-function mutants showed impaired induction of defense genes following exogenous ET treatment and increased susceptibility to Fusarium oxysporum”
Claim · model-written summary
Expression of ERF1 and AtERF2 depended on AtERF14 expression.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae0
- Gene
- AtERF14e1
- Outcome
- expression of other ERF genes involved in defense and ET/jasmonic acid responses, such as ERF1 and AtERF2e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“the expression of other ERF genes involved in defense and ET/jasmonic acid responses, such as ERF1 and AtERF2, depends on AtERF14 expression”
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