Abstract
Plants have evolved an array of adaptive responses to low Pi availability, a process modulated by various external stimuli and endogenous growth regulatory signals. Little is known about how these signaling processes interact to produce an integrated response. Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1) encodes a conserved MYB-type transcription factor that is essential for programming Pi starvation-induced gene expression and downstream Pi starvation responses (PSRs). Here, we show that loss-of-function mutations in FHY3 and FAR1, encoding two positive regulators of phytochrome signaling, and in EIN3, encoding a master regulator of ethylene responses, cause attenuated PHR1 expression, whereas mutation in HY5, encoding another positive regulator of light signaling, causes increased PHR1 expression. FHY3, FAR1, HY5, and EIN3 directly bind to the PHR1 promoter through distinct cis-elements. FHY3, FAR1, and EIN3 activate, while HY5 represses, PHR1 expression. FHY3 directly interacts with EIN3, and HY5 suppresses the transcriptional activation activity of FHY3 and EIN3 on PHR1. Finally, both light and ethylene promote FHY3 protein accumulation, and ethylene blocks the light-promoted stabilization of HY5. Our results suggest that light and ethylene coordinately regulate PHR1 expression and PSRs through signaling convergence at the PHR1 promoter.
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Claims 11
Claim · model-written summary
FHY3, FAR1 and EIN3 loss of function attenuated PHR1 expression.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- loss-of-function mutations in FHY3 and FAR1e1
- Gene
- PHR1e0
- Outcome
- PHR1 expressione1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“loss-of-function mutations in FHY3 and FAR1, encoding two positive regulators of phytochrome signaling, and in EIN3, encoding a master regulator of ethylene responses, cause attenuated PHR1 expression”
Claim · model-written summary
HY5 mutation increased PHR1 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- mutation in HY5e1
- Gene
- PHR1e0
- Outcome
- PHR1 expressione1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“mutation in HY5, encoding another positive regulator of light signaling, causes increased PHR1 expression”
Claim · model-written summary
FHY3, FAR1, HY5 and EIN3 directly bound distinct cis-elements of the PHR1 promoter.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- FHY3, FAR1, HY5, and EIN3e1
- Outcome
- bind to the PHR1 promotere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“FHY3, FAR1, HY5, and EIN3 directly bind to the PHR1 promoter through distinct cis-elements”
Claim · model-written summary
FHY3, FAR1 and EIN3 activated PHR1 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- FHY3, FAR1, and EIN3e1
- Outcome
- PHR1 expressione1
- Direction
- activatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“FHY3, FAR1, and EIN3 activate, while HY5 represses, PHR1 expression”
Claim · model-written summary
HY5 repressed PHR1 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- HY5e1
- Outcome
- PHR1 expressione1
- Direction
- repressese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“HY5 represses, PHR1 expression”
Claim · model-written summary
FHY3 directly interacted with EIN3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- FHY3e1
- Outcome
- interacts with EIN3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“FHY3 directly interacts with EIN3”
Claim · model-written summary
HY5 suppressed FHY3 and EIN3 transcriptional activation of PHR1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- HY5e1
- Outcome
- transcriptional activation activity of FHY3 and EIN3 on PHR1e1
- Direction
- suppressese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“HY5 suppresses the transcriptional activation activity of FHY3 and EIN3 on PHR1”
Claim · model-written summary
Light promoted FHY3 protein accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- lighte1
- Gene
- FHY3e1
- Outcome
- FHY3 protein accumulatione1
- Direction
- promotee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“both light and ethylene promote FHY3 protein accumulation”
Claim · model-written summary
Ethylene promoted FHY3 protein accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- ethylenee1
- Gene
- FHY3e1
- Outcome
- FHY3 protein accumulatione1
- Direction
- promotee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“both light and ethylene promote FHY3 protein accumulation”
Claim · model-written summary
Ethylene blocked light-promoted HY5 stabilization.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- ethylenee1
- Gene
- HY5e1
- Outcome
- light-promoted stabilization of HY5e1
- Direction
- blockse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“ethylene blocks the light-promoted stabilization of HY5”
Claim · model-written summary
The authors infer coordinated light and ethylene regulation of PHR1 expression and phosphate starvation responses through the PHR1 promoter.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- PHR1e1
- Outcome
- PHR1 expression and PSRse1
- Qualifier
- signaling convergence at the PHR1 promotere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana PHOSPHATE STARVATION RESPONSE1 (PHR1)”
e1“light and ethylene coordinately regulate PHR1 expression and PSRs through signaling convergence at the PHR1 promoter”
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