Abstract
Guard cell protoplasts of Vicia faba treated with 10 [mu]M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 [mu]m. Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation. Concomitantly, levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased 20% compared to levels in control cells; levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol did not change significantly in response to ABA treatment. These results show that phosphoinositide turnover is activated in response to ABA in guard cells. We conclude that phosphoinositide signaling is likely to be a step in the biochemical cascade that couples ABA to guard cell shrinking and stomatal closure.
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Claims 6
Claim · model-written summary
Guard cell protoplasts of Vicia faba treated with 10 uM (+)ABA in the light shrank by 20% in diameter within 1.5 h, from 24.1 to 19.6 um.
- Taxon
- Vicia faba
Vicia fabae1 - Organ / stage
- Guard cell protoplastse1
- Treatment
- 10 [mu]M (+)abscisic acid (ABA)e1
- Outcome
- diametere1
- Direction
- decreasee1
- Value
- 20%e1
- Qualifier
- within 1.5 h, from 24.1 to 19.6 [mu]me1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Guard cell protoplasts of Vicia faba treated with 10 [mu]M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 [mu]m”
Claim · model-written summary
Within 10 s of ABA administration, inositol 1,4,5-trisphosphate levels rose by 90%, provided cells had been treated with Li+ beforehand.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- administration of ABAe1
- Outcome
- levels of inositol 1,4,5-trisphosphatee1
- Direction
- increasee1
- Value
- 90%e1
- Qualifier
- provided that cells were treated with Li+e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e1“Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation”
Claim · model-written summary
Levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased by 20% compared with control cells after ABA.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- ABAe2
- Outcome
- levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphatee1
- Direction
- decreasede1
- Value
- 20%e1
- Comparator
- control cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e1“levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased 20% compared to levels in control cells”
e2“administration of ABA”
Claim · model-written summary
Label levels in phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol did not change significantly with ABA treatment.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- ABA treatmente1
- Outcome
- levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerole1
- Direction
- did not change significantlye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e1“levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol did not change significantly in response to ABA treatment”
Claim · model-written summary
The authors conclude that phosphoinositide turnover is activated by ABA in guard cells.
- Taxon
- Vicia fabaVicia fabat0
- Organ / stage
- guard cellse1
- Treatment
- ABAe1
- Outcome
- phosphoinositide turnovere1
- Direction
- activatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e1“phosphoinositide turnover is activated in response to ABA in guard cells”
Claim · model-written summary
The authors conclude that phosphoinositide signaling is likely a step in the cascade coupling ABA to guard cell shrinking and stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- ABAe1
- Outcome
- guard cell shrinking and stomatal closuree1
- Qualifier
- phosphoinositide signaling is likely to be a step in the biochemical cascadee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vicia faba”
e1“phosphoinositide signaling is likely to be a step in the biochemical cascade that couples ABA to guard cell shrinking and stomatal closure”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Vicia faba (faba bean)Results differ
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.