Paper · 2009 · Genetics and Molecular Research

Salt stress causes a shift in the localization pattern of germin gene expression

M. Caliskan

2
claims
4
verbatim quotes
14
citations
2
research areas
✓
Open access

Abstract

The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of plant development, such as defense, embryonic development and response to biotic as well as abiotic stress, including salt. The responses of germin and germin-like genes to salt stress vary in different plants. RT-PCR and in situ RNA hybridization methods were employed to analyze quantitative changes and to detect the localization pattern of germin gene products in salt-stressed and water-grown wheat embryos. Salt stress caused no quantitative changes in the synthesis of germin gene products. However, in situ RNA hybridization revealed changes in the expression site of germin gene products. Coleorhiza cells were found to be the site of germin accumulation in water-grown embryos; however, in salt-stressed embryos, germin mRNA accumulated in coleoptile cells instead of coleorhiza tissue.

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Claims 2

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Salt stress caused no quantitative change in germin synthesis in wheat embryos.

Organ / stage
embryose1
Treatment
Salt stresse0
Gene
germine0
Outcome
synthesis of germin gene productse0
Direction
no quantitative changese0
Comparator
water-growne1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Salt stress caused no quantitative changes in the synthesis of germin gene products”

✓ located verbatim in the source · abstract, characters 741–824 · show in text ↑
e1

“salt-stressed and water-grown wheat embryos”

✓ located verbatim in the source · abstract, characters 696–739 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Salt stress shifted germin mRNA accumulation from coleorhiza to coleoptile cells in wheat embryos.

Organ / stage
coleoptile cellse0
Treatment
salt-stressede0
Gene
germine0
Outcome
germin mRNA accumulated in coleoptile cells instead of coleorhiza tissuee0
Comparator
coleorhiza tissuee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“in salt-stressed embryos, germin mRNA accumulated in coleoptile cells instead of coleorhiza tissue”

✓ located verbatim in the source · abstract, characters 1025–1123 · show in text ↑
e1

“wheat embryos”

✓ located verbatim in the source · abstract, characters 726–739 · show in text ↑

salinity · molecular: expression · Triticum aestivum (wheat)Results differ

39 papers: 22 positive, 17 negative or no effect

16 papers in the same direction as this paper · 22 in the opposite direction

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.