Abstract
This study was conducted to develop an optimized embryogenesis and plantlet regeneration protocol for wheat (Triticum aestivum L.) cultivar Khaleefa. The effects of sucrose concentrations, activated charcoal, potassium phosphate, ammonium nitrate, potassium nitrate, chelated iron, different concentrations of benzyladenine (BAP), kinetin (KN) and 5phenylcarbamoylamino (TDZ) in combination with indole-3-acetic acid (IAA) and different concentrations of silver nitrate were evaluated. Results showed that adding sucrose at (6%), activated charcoal (AC) at 0.5 g -1 and the normal concentrations of potassium phosphate, ammonium nitrate, potassium nitrate and chelated iron in Murashige and Skoog basal medium (MS) salt were the best for promoting embryogenesis. Adding 2.0 mg -1 BA in combination with 1.0 mg -1 IAA gave the highest value of somatic embryos formation compared with other concentrations tested and addition of 20.0 mg -1 silver nitrate to regeneration medium encouraged shoot regeneration.
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Claims 5
Claim · model-written summary
Six percent sucrose best promoted wheat embryogenesis.
- Taxon
- wheat (Triticum aestivum L.)
Triticum aestivumt0 - Treatment
- sucrose at (6%)e0
- Outcome
- embryogenesise0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adding sucrose at (6%), activated charcoal (AC) at 0.5 g -1 and the normal concentrations of potassium phosphate, ammonium nitrate, potassium nitrate and chelated iron in Murashige and Skoog basal medium (MS) salt were the best for promoting embryogenesis”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
Activated charcoal at 0.5 g -1 best promoted wheat embryogenesis.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- activated charcoal (AC) at 0.5 g -1e0
- Outcome
- embryogenesise0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activated charcoal (AC) at 0.5 g -1 and the normal concentrations of potassium phosphate, ammonium nitrate, potassium nitrate and chelated iron in Murashige and Skoog basal medium (MS) salt were the best for promoting embryogenesis”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
Normal MS salt concentrations best promoted embryogenesis.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- normal concentrations of potassium phosphate, ammonium nitrate, potassium nitrate and chelated irone0
- Outcome
- embryogenesise0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the normal concentrations of potassium phosphate, ammonium nitrate, potassium nitrate and chelated iron in Murashige and Skoog basal medium (MS) salt were the best for promoting embryogenesis”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
BA plus IAA gave the highest somatic embryo formation among tested concentrations.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- 2.0 mg -1 BA in combination with 1.0 mg -1 IAAe0
- Outcome
- somatic embryos formatione0
- Direction
- higheste0
- Comparator
- other concentrations testede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding 2.0 mg -1 BA in combination with 1.0 mg -1 IAA gave the highest value of somatic embryos formation compared with other concentrations tested”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
Silver nitrate at 20.0 mg -1 encouraged shoot regeneration.
- Taxon
- wheat (Triticum aestivum L.)Triticum aestivumt0
- Treatment
- 20.0 mg -1 silver nitratee0
- Outcome
- shoot regeneratione0
- Direction
- encouragede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“addition of 20.0 mg -1 silver nitrate to regeneration medium encouraged shoot regeneration”
t0“wheat (Triticum aestivum L.)”
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.