Abstract
Abstract We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos. Firstly, we established the optimal combination of basal media, inoculation method and pretreatment method using biostatistical methods. The results indicated that the combination of MS medium and longitudinally bisected mature embryos showed the highest culture efficiency, whereas the pretreatment method had no significant effects on callus induction or plant regeneration. A 70% primary callus induction rate was achieved on MS medium containing 2 mg/l 2,4‐ d for all tested cultivars. Primary calli were then transferred onto the subculture medium to initiate embryogenic calli. Supplementation of the subculture medium with the appropriate combination of phytohormones (2.0 mg/l 2,4‐ d , 0.5 mg/l BA and 0.1 mg/l NAA) significantly enhanced embryogenic callus production. The addition of AgNO 3 (10 mg/l) in regeneration medium promoted plant regeneration, whereas CuSO 4 stimulated root formation. The use of this protocol achieved successful plant regeneration in eight tested cultivars. The culture efficiency ranged from 15.3% to 36.8%, suggesting this regeneration system may be an effective alternative for wheat genetic transformation.
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Claims 9
Claim · model-written summary
The combination of MS medium and longitudinally bisected mature embryos gave the highest culture efficiency.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- combination of MS medium and longitudinally bisected mature embryose1
- Outcome
- culture efficiencye1
- Direction
- higheste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e1“The results indicated that the combination of MS medium and longitudinally bisected mature embryos showed the highest culture efficiency”
Claim · model-written summary
The pretreatment method had no significant effect on callus induction or plant regeneration in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- pretreatment methode2
- Outcome
- callus induction or plant regeneratione2
- Direction
- no significant effectse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e2“the pretreatment method had no significant effects on callus induction or plant regeneration”
Claim · model-written summary
A 70% primary callus induction rate was achieved on MS medium containing 2 mg/l 2,4-D for all tested wheat cultivars.
- Taxon
- wheatTriticum aestivume0
- Material
- all tested cultivarse3
- Treatment
- MS medium containing 2 mg/l 2,4‐ de3
- Outcome
- primary callus induction ratee3
- Value
- 70%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e3“A 70% primary callus induction rate was achieved on MS medium containing 2 mg/l 2,4‐ d for all tested cultivars”
Claim · model-written summary
Phytohormone supplementation of the subculture medium significantly enhanced embryogenic callus production in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 2.0 mg/l 2,4‐ d , 0.5 mg/l BA and 0.1 mg/l NAAe4
- Outcome
- embryogenic callus productione4
- Direction
- significantly enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e4“Supplementation of the subculture medium with the appropriate combination of phytohormones (2.0 mg/l 2,4‐ d , 0.5 mg/l BA and 0.1 mg/l NAA) significantly enhanced embryogenic callus production”
Claim · model-written summary
AgNO 3 (10 mg/l) in regeneration medium promoted plant regeneration in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- AgNO 3 (10 mg/l)e5
- Outcome
- plant regeneratione5
- Direction
- promotede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e5“The addition of AgNO 3 (10 mg/l) in regeneration medium promoted plant regeneration”
Claim · model-written summary
CuSO 4 stimulated root formation in wheat regeneration.
- Taxon
- wheatTriticum aestivume0
- Treatment
- CuSO 4e6
- Outcome
- root formatione6
- Direction
- stimulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e6“CuSO 4 stimulated root formation”
Claim · model-written summary
The protocol achieved successful plant regeneration in eight tested wheat cultivars.
- Taxon
- wheatTriticum aestivume0
- Material
- eight tested cultivarse7
- Outcome
- plant regeneratione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e7“The use of this protocol achieved successful plant regeneration in eight tested cultivars”
Claim · model-written summary
Culture efficiency in wheat ranged from 15.3% to 36.8%.
- Taxon
- wheatTriticum aestivume0
- Outcome
- culture efficiencye7
- Value
- 15.3% to 36.8%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e7“The culture efficiency ranged from 15.3% to 36.8%”
Claim · model-written summary
The regeneration system may be an effective alternative for wheat genetic transformation.
- Taxon
- wheatTriticum aestivume0
- Outcome
- effective alternative for wheat genetic transformatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos”
e6“suggesting this regeneration system may be an effective alternative for wheat genetic transformation”
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