Abstract
Selenium (Se) is an essential element for many organisms but is toxic at higher levels. CpNifS is a chloroplastic NifS-like protein in Arabidopsis (Arabidopsis thaliana) that can catalyze the conversion of cysteine into alanine and elemental sulfur (S0) and of selenocysteine into alanine and elemental Se (Se0). We overexpressed CpNifS to investigate the effects on Se metabolism in plants. CpNifS overexpression significantly enhanced selenate tolerance (1.9-fold) and Se accumulation (2.2-fold). CpNifS overexpressors showed significantly reduced Se incorporation into protein, which may explain their higher Se tolerance. Also, sulfur accumulation was enhanced by approximately 30% in CpNifS overexpressors, both on media with and without selenate. Root transcriptome changes in response to selenate mimicked the effects observed under sulfur starvation. There were only a few transcriptome differences between CpNifS-overexpressing plants and wild type, besides the 25- to 40-fold increase in CpNifS levels. Judged from x-ray analysis of near edge spectrum, both CpNifS overexpressors and wild type accumulated mostly selenate (Se(VI)). In conclusion, overexpression of this plant NifS-like protein had a pronounced effect on plant Se metabolism. The observed enhanced Se accumulation and tolerance of CpNifS overexpressors show promise for use in phytoremediation.
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Claims 9
Claim · model-written summary
CpNifS overexpression significantly enhanced selenate tolerance by 1.9-fold.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- CpNifS overexpressione0
- Gene
- CpNifSe0
- Outcome
- selenate tolerancee0
- Direction
- enhancede0
- Value
- 1.9-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CpNifS overexpression significantly enhanced selenate tolerance (1.9-fold) and Se accumulation (2.2-fold).”
t0“Arabidopsis”
Claim · model-written summary
CpNifS overexpression significantly enhanced selenium accumulation by 2.2-fold.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- CpNifS overexpressione0
- Gene
- CpNifSe0
- Outcome
- Se accumulatione0
- Direction
- enhancede0
- Value
- 2.2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CpNifS overexpression significantly enhanced selenate tolerance (1.9-fold) and Se accumulation (2.2-fold).”
t0“Arabidopsis”
Claim · model-written summary
CpNifS overexpressors had significantly reduced selenium incorporation into protein.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- CpNifS overexpressorse0
- Outcome
- Se incorporation into proteine0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CpNifS overexpressors showed significantly reduced Se incorporation into protein”
t0“Arabidopsis”
Claim · model-written summary
Sulfur accumulation was enhanced by approximately 30% in CpNifS overexpressors with and without selenate.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- CpNifS overexpressorse0
- Outcome
- sulfur accumulatione0
- Direction
- enhancede0
- Value
- approximately 30%e0
- Qualifier
- both on media with and without selenatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sulfur accumulation was enhanced by approximately 30% in CpNifS overexpressors, both on media with and without selenate”
t0“Arabidopsis”
Claim · model-written summary
The root transcriptome response to selenate resembled sulfur starvation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- selenatee0
- Outcome
- Root transcriptome changese0
- Comparator
- sulfur starvatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root transcriptome changes in response to selenate mimicked the effects observed under sulfur starvation.”
t0“Arabidopsis”
Claim · model-written summary
Only a few transcriptome differences distinguished CpNifS overexpressors from wild type.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CpNifSe0
- Outcome
- transcriptome differencese0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were only a few transcriptome differences between CpNifS-overexpressing plants and wild type, besides the 25- to 40-fold increase in CpNifS levels.”
t0“Arabidopsis”
Claim · model-written summary
CpNifS levels increased 25- to 40-fold in overexpressors relative to wild type.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CpNifSe0
- Outcome
- CpNifS levelse0
- Direction
- increasee0
- Value
- 25- to 40-folde0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were only a few transcriptome differences between CpNifS-overexpressing plants and wild type, besides the 25- to 40-fold increase in CpNifS levels.”
t0“Arabidopsis”
Claim · model-written summary
Both overexpressors and wild type accumulated mostly selenate.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- accumulated mostly selenate (Se(VI))e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both CpNifS overexpressors and wild type accumulated mostly selenate (Se(VI))”
t0“Arabidopsis”
Claim · model-written summary
The enhanced accumulation and tolerance of overexpressors show promise for phytoremediation.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- use in phytoremediatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The observed enhanced Se accumulation and tolerance of CpNifS overexpressors show promise for use in phytoremediation.”
t0“Arabidopsis”