Paper · 1977 · PLANT PHYSIOLOGY

Tannins as Gibberellin Antagonists in the Synthesis of α-Amylase and Acid Phosphatase by Barley Seeds

Andrew Jacobson; Mary Ritzel Corcoran

7
claims
11
verbatim quotes
26
citations
1
research areas
✓
Open access

Abstract

The tannins chebulinic acid or tara tannin were added to an incubation system in which GA(3) induces enzyme synthesis in endosperm half seeds of barley (Hordeum vulgare L.). The activity of amylase and acid phosphatase in the incubation medium was reduced compared to the activity in the medium after incubation with GA(3) alone. When embryo half seeds of barley were incubated with chebulinic acid or tara tannin in the absence of added GA(3), the enzyme activity of the incubation medium was also reduced. The activity of preformed enzymes obtained from endosperm half seeds previously induced with GA(3) was not reduced by the addition of tannin. Comparisons were made of the amount of enzyme activity from breis of aleurone layers incubated with GA(3) in the presence and absence of tannins. The amounts of activity were relatively small and approximately equal in both cases, indicating that secretion from the aleurone was not blocked by the tannins. The reduction of enzyme activity caused by tannins in both endosperm and embryo half seeds could be completely reversed by the addition of GA(3).

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chebulinic acid reduced amylase and acid phosphatase activity in barley endosperm medium relative to GA(3) alone.

Treatment
chebulinic acide0
Outcome
activity of amylase and acid phosphatasee0
Direction
reducede0
Comparator
GA(3) alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The tannins chebulinic acid or tara tannin were added to an incubation system in which GA(3) induces enzyme synthesis in endosperm half seeds of barley (Hordeum vulgare L.). The activity of amylase and acid phosphatase in the incubation medium was reduced compared to the activity in the medium after incubation with GA(3) alone.”

✓ located verbatim in the source · abstract, characters 0–329 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Tara tannin reduced amylase and acid phosphatase activity in barley endosperm medium relative to GA(3) alone.

Treatment
tara tannine0
Outcome
activity of amylase and acid phosphatasee0
Direction
reducede0
Comparator
GA(3) alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The tannins chebulinic acid or tara tannin were added to an incubation system in which GA(3) induces enzyme synthesis in endosperm half seeds of barley (Hordeum vulgare L.). The activity of amylase and acid phosphatase in the incubation medium was reduced compared to the activity in the medium after incubation with GA(3) alone.”

✓ located verbatim in the source · abstract, characters 0–329 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chebulinic acid reduced enzyme activity in embryo half seeds without added GA(3).

Organ / stage
embryo half seedse0
Treatment
chebulinic acide0
Outcome
enzyme activity of the incubation mediume0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“When embryo half seeds of barley were incubated with chebulinic acid or tara tannin in the absence of added GA(3), the enzyme activity of the incubation medium was also reduced.”

✓ located verbatim in the source · abstract, characters 330–507 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Tara tannin reduced enzyme activity in embryo half seeds without added GA(3).

Organ / stage
embryo half seedse0
Treatment
tara tannine0
Outcome
enzyme activity of the incubation mediume0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“When embryo half seeds of barley were incubated with chebulinic acid or tara tannin in the absence of added GA(3), the enzyme activity of the incubation medium was also reduced.”

✓ located verbatim in the source · abstract, characters 330–507 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tannin did not reduce activity of preformed enzymes in barley endosperm half seeds.

Taxon
Barley SeedsHordeum vulgaret0
Treatment
addition of tannine0
Outcome
activity of preformed enzymese0
Direction
not reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The activity of preformed enzymes obtained from endosperm half seeds previously induced with GA(3) was not reduced by the addition of tannin.”

✓ located verbatim in the source · abstract, characters 508–649 · show in text ↑
t0

“Barley Seeds”

✓ located verbatim in the source · title, characters 89–101 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Aleurone enzyme activity was approximately equal with and without tannins, suggesting secretion was not blocked.

Taxon
Barley SeedsHordeum vulgaret0
Treatment
presence and absence of tanninse1
Outcome
amounts of activitye0
Direction
approximately equale0
Comparator
absence of tanninse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The amounts of activity were relatively small and approximately equal in both cases, indicating that secretion from the aleurone was not blocked by the tannins.”

✓ located verbatim in the source · abstract, characters 796–956 · show in text ↑
e1

“aleurone layers incubated with GA(3) in the presence and absence of tannins”

✓ located verbatim in the source · abstract, characters 719–794 · show in text ↑
t0

“Barley Seeds”

✓ located verbatim in the source · title, characters 89–101 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding GA(3) completely reversed the tannin-induced reduction of enzyme activity in barley endosperm and embryo halves.

Taxon
Barley SeedsHordeum vulgaret0
Treatment
addition of GA(3)e0
Outcome
reduction of enzyme activitye0
Direction
completely reversede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The reduction of enzyme activity caused by tannins in both endosperm and embryo half seeds could be completely reversed by the addition of GA(3).”

✓ located verbatim in the source · abstract, characters 957–1102 · show in text ↑
t0

“Barley Seeds”

✓ located verbatim in the source · title, characters 89–101 · show in text ↑

Gibberellic acid · soil: biology · Hordeum vulgare (barley)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.