Abstract
Root-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita. In roots containing a GUS reporter fusion to the Hahsp17.7G4 promoter, 10% of the galls stained for GUS expression 1 to 3 days after infection and the fraction stained increased to 60 to 80% 17 to 20 days after infection. A DNA fragment from -83 to +163, which contains heat-shock element (HSE) core sequences, is sufficient to support a promoter activity largely restricted to giant cells within the galls. Two-point mutations in HSE cores, previously reported to abolish the heat-shock response and to strongly reduce the embryogenesis response of the same promoter, did not reduce expression in giant cells. This suggests a distinct regulation of the promoter by nematodes. However, additional point mutations located at positions crucial for binding of heat-shock transcription factors (HSFs) caused a severe decrease in the nematode response. These results demonstrate that HSEs are involved in the promoter activation in giant cells and suggest that HSFs may mediate this response.
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Claims 9
Claim · model-written summary
The Hahsp17.7G4 promoter directed GUS expression in tobacco galls induced by Meloidogyne incognita.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- gallse2
- Interactor
- Meloidogyne incognita
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogyne incognitae2pest - Gene
- Hahsp17.7G4e1
- Outcome
- GUS expressione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“tobacco galls”
e1“promoter of the Hahsp17.7G4 gene”
e2“directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita”
Claim · model-written summary
10% of the galls stained for GUS expression 1 to 3 days after infection.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- galls stained for GUS expressione1
- Value
- 10%e1
- Qualifier
- 1 to 3 days after infectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“10% of the galls stained for GUS expression 1 to 3 days after infection”
Claim · model-written summary
The fraction of galls stained for GUS increased to 60 to 80% 17 to 20 days after infection.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- fraction stainede1
- Direction
- increasede1
- Value
- 60 to 80%e1
- Qualifier
- 17 to 20 days after infectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“the fraction stained increased to 60 to 80% 17 to 20 days after infection”
Claim · model-written summary
A DNA fragment from -83 to +163 containing HSE core sequences is sufficient for promoter activity largely restricted to giant cells within the galls.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- DNA fragment from -83 to +163e1
- Organ / stage
- giant cellse1
- Outcome
- promoter activity largely restricted to giant cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“A DNA fragment from -83 to +163, which contains heat-shock element (HSE) core sequences, is sufficient to support a promoter activity largely restricted to giant cells within the galls”
Claim · model-written summary
Two-point mutations in HSE cores did not reduce expression in giant cells.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- giant cellse1
- Treatment
- Two-point mutations in HSE corese1
- Outcome
- expression in giant cellse1
- Direction
- did not reducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“Two-point mutations in HSE cores, previously reported to abolish the heat-shock response and to strongly reduce the embryogenesis response of the same promoter, did not reduce expression in giant cells”
Claim · model-written summary
Additional point mutations at positions crucial for binding of heat-shock transcription factors caused a severe decrease in the nematode response.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- additional point mutations located at positions crucial for binding of heat-shock transcription factorse1
- Outcome
- nematode responsee1
- Direction
- severe decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“additional point mutations located at positions crucial for binding of heat-shock transcription factors (HSFs) caused a severe decrease in the nematode response”
Claim · model-written summary
HSEs are involved in the promoter activation in giant cells.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- HSEse1
- Outcome
- promoter activation in giant cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“These results demonstrate that HSEs are involved in the promoter activation in giant cells”
Claim · model-written summary
HSFs may mediate the promoter response in giant cells.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- HSFse1
- Outcome
- this responsee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“suggest that HSFs may mediate this response”
Claim · model-written summary
The authors suggest a distinct regulation of the promoter by nematodes.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- distinct regulation of the promotere1
- Qualifier
- by nematodese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco galls”
e1“This suggests a distinct regulation of the promoter by nematodes”
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