Abstract
Efficient shoot bud formation (94.5 ± 7.59), in vitro regeneration and production of flowers were obtained from sterile plants of Begonia x hiemalis Fotsch. An in vitro regeneration was attempted using immature reproductive organs, which were not commonly used before, such as young inflorescences, peduncles and petals of flowers collected in the field. The flower segments were cultured on solid Murashige and Skoog (MS) medium, supplemented with various concentrations and combinations of plant growth regulators (PGRs) and adenine. Within 8 weeks of the culture period, the highest frequency of reproductive shoot regeneration (red calyx, 8.50) was obtained from explants of immature inflorescence cultured on the MS medium supplemented with 1.0 mgL-1 benzyl amino purine (BA) and 1.0 mgL-1 1-naphthalene acetic acid (NAA), added with 40 mgL-1 adenine and 3 sucrose. To attain further root growth and maturity, the clonal plantlets (with or without reproductive shoots) were excised and sub-cultured onto the MS medium fortified with 1.0 mgL-1 Gibberellic acid (GA3). 87 of the regenerated plantlets were successfully acclimatized on 2:1:1 ratio of peat: soil: sand, under greenhouse conditions. All of them were capable of producing true-to-type flowers. The in vitro developed reproductive shoots, also further generated complete, small flowers, which were morphologically similar to the in vivo types, following an ex vitro acclimatization.This study provides an alternative approach to generate early flowering in Begonia without undergoing the full growth cycle and hence can help overcome problems associated with flower growth and development in this species. The present study revealed that floral parts (inflorescence, peduncle and petals) could also be used as a source of explants besides the commonly used tissues such as leaf, stem, shoot and root segments.
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Claims 6
Claim · model-written summary
Shoot bud formation in Begonia x hiemalis was efficient, at 94.5.
- Taxon
- Begonia x hiemalis fotsch
Begonia ×hiemalist0 - Outcome
- shoot bud formatione1
- Value
- 94.5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“Efficient shoot bud formation (94.5”
Claim · model-written summary
The highest frequency of reproductive shoot regeneration (red calyx, 8.50) was obtained within 8 weeks from immature inflorescence explants on MS medium with 1.0 mg/L BA, 1.0 mg/L NAA, 40 mg/L adenine and 3 sucrose.
- Taxon
- Begonia x hiemalis fotschBegonia ×hiemalist0
- Organ / stage
- immature inflorescencee1
- Treatment
- MS medium supplemented with 1.0 mgL-1 benzyl amino purine (BA) and 1.0 mgL-1 1-naphthalene acetic acid (NAA), added with 40 mgL-1 adenine and 3 sucrosee1
- Outcome
- reproductive shoot regeneratione1
- Direction
- higheste1
- Value
- 8.50e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“the highest frequency of reproductive shoot regeneration (red calyx, 8.50) was obtained from explants of immature inflorescence cultured on the MS medium supplemented with 1.0 mgL-1 benzyl amino purine (BA) and 1.0 mgL-1 1-naphthalene acetic acid (NAA), added with 40 mgL-1 adenine and 3 sucrose”
Claim · model-written summary
87 of the regenerated plantlets were successfully acclimatized in peat:soil:sand 2:1:1 under greenhouse conditions.
- Taxon
- Begonia x hiemalis fotschBegonia ×hiemalist0
- Organ / stage
- regenerated plantletse1
- Outcome
- successfully acclimatizede1
- Value
- 87e1
- Setting
- greenhouse conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“87 of the regenerated plantlets were successfully acclimatized on 2:1:1 ratio of peat: soil: sand, under greenhouse conditions”
Claim · model-written summary
All acclimatized regenerated plantlets were capable of producing true-to-type flowers.
- Taxon
- Begonia x hiemalis fotschBegonia ×hiemalist0
- Outcome
- true-to-type flowerse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“All of them were capable of producing true-to-type flowers”
Claim · model-written summary
In vitro developed reproductive shoots generated complete, small flowers morphologically similar to in vivo flowers after ex vitro acclimatization.
- Taxon
- Begonia x hiemalis fotschBegonia ×hiemalist0
- Organ / stage
- reproductive shootse1
- Outcome
- complete, small flowerse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“The in vitro developed reproductive shoots, also further generated complete, small flowers, which were morphologically similar to the in vivo types”
Claim · model-written summary
The study concludes that floral parts (inflorescence, peduncle and petals) can be used as a source of explants.
- Taxon
- Begonia x hiemalis fotschBegonia ×hiemalist0
- Organ / stage
- floral partse1
- Outcome
- source of explantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Begonia x hiemalis fotsch”
e1“floral parts (inflorescence, peduncle and petals) could also be used as a source of explants”
The same questions in other papers
1-naphthaleneacetic acid · growth · Begonia x hiemalis (winter-flowering begonia)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Begonia x hiemalis (winter-flowering begonia)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Begonia x hiemalis (winter-flowering begonia)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.