Abstract
BRCA1 is a well-known tumor suppressor protein in mammals, involved in multiple cellular processes such as DNA repair, chromosome segregation and chromatin remodeling. Interestingly, homologs of BRCA1 and several of its complex partners are also found in plants. As the respective mutants are viable, in contrast to mammalian mutants, detailed analyses of their biological role is possible. Here we demonstrate that the model plant Arabidopsis thaliana harbors two homologs of the mammalian BRCA1 interaction partner BRCC36, AtBRCC36A and AtBRCC36B. Mutants of both genes as well as the double mutants are fully fertile and show no defects in development. We were able to show that mutation of one of the homologs, AtBRCC36A, leads to a severe defect in intra- and interchromosomal homologous recombination (HR). A HR defect is also apparent in Atbrca1 mutants. As the Atbrcc36a/Atbrca1 double mutant behaves like the single mutants of AtBRCA1 and AtBRCC36A both proteins seem to be involved in a common pathway in the regulation of HR. AtBRCC36 is also epistatic to AtBRCA1 in DNA crosslink repair. Upon genotoxic stress, AtBRCC36A is transferred into the nucleus.
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Claims 6
Claim · model-written summary
Mutants of AtBRCC36A, AtBRCC36B and the double mutant are fully fertile and show no developmental defects.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Outcome
- defects in developmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“Mutants of both genes as well as the double mutants are fully fertile and show no defects in development”
Claim · model-written summary
Mutation of AtBRCC36A leads to a severe defect in intra- and interchromosomal homologous recombination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- mutation of one of the homologse2
- Gene
- AtBRCC36Ae2
- Outcome
- intra- and interchromosomal homologous recombination (HR)e2
- Direction
- severe defecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“mutation of one of the homologs, AtBRCC36A, leads to a severe defect in intra- and interchromosomal homologous recombination (HR)”
Claim · model-written summary
A homologous recombination defect is also apparent in Atbrca1 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Atbrca1 mutantse3
- Outcome
- HR defecte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“A HR defect is also apparent in Atbrca1 mutants”
Claim · model-written summary
The Atbrcc36a/Atbrca1 double mutant behaves like the single mutants, suggesting both proteins act in a common pathway regulating homologous recombination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Atbrcc36a/Atbrca1 double mutante4
- Outcome
- regulation of HRe4
- Qualifier
- a common pathwaye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“As the Atbrcc36a/Atbrca1 double mutant behaves like the single mutants of AtBRCA1 and AtBRCC36A both proteins seem to be involved in a common pathway in the regulation of HR”
Claim · model-written summary
AtBRCC36 is epistatic to AtBRCA1 in DNA crosslink repair.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtBRCC36e5
- Outcome
- DNA crosslink repaire5
- Direction
- epistatice5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e5“AtBRCC36 is also epistatic to AtBRCA1 in DNA crosslink repair”
Claim · model-written summary
Upon genotoxic stress, AtBRCC36A is transferred into the nucleus in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- genotoxic stresse6
- Gene
- AtBRCC36Ae6
- Outcome
- transferred into the nucleuse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e6“Upon genotoxic stress, AtBRCC36A is transferred into the nucleus”
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