Paper · 1989 · New Phytologist

Appropriate controls for vesicular–arbuscular mycorrhiza research

ROGER T. KOIDE; MINGGUANG LI

9
claims
16
verbatim quotes
242
citations
2
research areas
✓
Open access

Abstract

SUMMARY Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum. Use of inocula containing mycorrhizal propagules ultimately resulted in growth promotions which were related to improved phosphorus nutrition. Inoculation with soil containing mycorrhizal propagules caused an initial growth depression in comparison with plants receiving no inoculation, but inoculation with washed spores did not. For several reasons, the growth depression caused by inoculation with soil was not attributable to mycorrhizal infection. First, the growth depression was evident before mycorrhizal infections had become established. Second, the growth depression was also evident when either soil or soil sievings, both lacking mycorrhizal propagules, were used. Third, similar growth depressions were observed in mustard. The results suggest that careful selection of appropriate controls for mycorrhizal plants must be made, particularly if the emphasis is on early phases of plant growth. Use of non‐sterile soil reduced the extent of mycorrhizal infection.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inocula containing mycorrhizal propagules promoted growth of sunflower and mustard, related to improved phosphorus nutrition.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Treatment
inocula containing mycorrhizal propagulese1
Compound
phosphoruse1
Outcome
growthe1
Direction
promotionse1
Qualifier
related to improved phosphorus nutritione1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e1

“Use of inocula containing mycorrhizal propagules ultimately resulted in growth promotions which were related to improved phosphorus nutrition”

✓ located verbatim in the source · abstract, characters 173–314 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Inoculation with soil containing mycorrhizal propagules caused an initial growth depression relative to no inoculation.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Treatment
Inoculation with soil containing mycorrhizal propagulese2
Outcome
growthe2
Direction
depressione2
Qualifier
initiale2
Comparator
plants receiving no inoculatione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e2

“Inoculation with soil containing mycorrhizal propagules caused an initial growth depression in comparison with plants receiving no inoculation, but inoculation with washed spores did not”

✓ located verbatim in the source · abstract, characters 316–502 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Inoculation with washed spores did not cause an initial growth depression relative to no inoculation.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Treatment
inoculation with washed sporese2
Outcome
growthe2
Direction
did note2
Comparator
plants receiving no inoculatione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e2

“Inoculation with soil containing mycorrhizal propagules caused an initial growth depression in comparison with plants receiving no inoculation, but inoculation with washed spores did not”

✓ located verbatim in the source · abstract, characters 316–502 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The growth depression caused by soil inoculation was not attributable to mycorrhizal infection.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Treatment
inoculation with soile3
Outcome
growth depressione3
Qualifier
not attributable to mycorrhizal infectione3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e3

“For several reasons, the growth depression caused by inoculation with soil was not attributable to mycorrhizal infection”

✓ located verbatim in the source · abstract, characters 504–624 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The growth depression was evident before mycorrhizal infections had become established.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Outcome
growth depressione4
Qualifier
before mycorrhizal infections had become establishede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e4

“First, the growth depression was evident before mycorrhizal infections had become established. Second, the growth depression was also evident when either soil or soil sievings, both lacking mycorrhizal propagules, were used. Third, similar growth depressions were observed in mustard”

✓ located verbatim in the source · abstract, characters 626–909 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The growth depression was also evident when soil or soil sievings lacking mycorrhizal propagules were used.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Outcome
growth depressione4
Qualifier
both lacking mycorrhizal propagulese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e4

“First, the growth depression was evident before mycorrhizal infections had become established. Second, the growth depression was also evident when either soil or soil sievings, both lacking mycorrhizal propagules, were used. Third, similar growth depressions were observed in mustard”

✓ located verbatim in the source · abstract, characters 626–909 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Similar growth depressions were observed in mustard.

Taxon
mustarde4
Outcome
growth depressionse4

Verbatim

e4

“First, the growth depression was evident before mycorrhizal infections had become established. Second, the growth depression was also evident when either soil or soil sievings, both lacking mycorrhizal propagules, were used. Third, similar growth depressions were observed in mustard”

✓ located verbatim in the source · abstract, characters 626–909 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Use of non-sterile soil reduced the extent of mycorrhizal infection.

Taxon
Sunflower ( Helianthus animus L.)Helianthus annuuse0
Treatment
Use of non‐sterile soile5
Outcome
extent of mycorrhizal infectione5
Direction
reducede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Sunflower ( Helianthus animus L.) and mustard ( Brassica hirta Moench.) plants were grown in autoclaved soil to which was added various components of soil inoculum”

✓ located verbatim in the source · abstract, characters 8–171 · show in text ↑
e5

“Use of non‐sterile soil reduced the extent of mycorrhizal infection”

✓ located verbatim in the source · abstract, characters 1080–1147 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Careful selection of appropriate controls must be made for mycorrhizal plants, particularly for early growth.

Taxon
mycorrhizal plantse6
Outcome
careful selection of appropriate controlse6
Qualifier
particularly if the emphasis is on early phases of plant growthe6

Verbatim

e6

“The results suggest that careful selection of appropriate controls for mycorrhizal plants must be made, particularly if the emphasis is on early phases of plant growth”

✓ located verbatim in the source · abstract, characters 911–1078 · show in text ↑

mycorrhiza · growth · Helianthus annuus (common sunflower)Positive and negative results

15 papers: 12 positive, 3 negative or no effect

11 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. no effect

11 papers more in this group

See all 15 papers →

inoculation · growth · Helianthus annuus (common sunflower)Positive and negative results

62 papers: 54 positive, 8 negative or no effect

53 papers in the same direction as this paper · 7 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

58 papers more in this group

See all 62 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.