Abstract
To study and induce meiotic recombination in plants, we generated and analyzed transgenic tomato hybrids F1-RecA and F1-NLS-recA-LicBM3 expressing, respectively, the recA gene of Escherichia coli and the NLS-recA-licBM3 gene. It was found that the recA and NLS-recA-licBM3 genes are inherited through the maternal and paternal lineages, they have no selective influence on the pollen and are contained in tomato F1-RecA and F1-NLS-RecA-LicBM3 hybrids outside the second chromosome in the hemizygous state. The comparative analysis of the meiotic recombination frequency (rf) in the progenies of the transgenic and nontransgenic hybrids showed that only the expression of the recA gene of E. coli in cells of the F1-RecA plants produced a 1.2-1.5-fold increase in the frequency of recombination between some linked marker genes of the second chromosome of tomato.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
The two transgenes were inherited through maternal and paternal lineages.
- Taxon
- tomato
Solanum lycopersicumt0 - Gene
- recA and NLS-recA-licBM3 genese0
- Outcome
- inherited through the maternal and paternal lineagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the recA and NLS-recA-licBM3 genes are inherited through the maternal and paternal lineages”
t0“tomato”
Claim · model-written summary
The transgenes had no selective effect on pollen.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- pollene0
- Outcome
- no selective influence on the pollene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they have no selective influence on the pollen”
t0“tomato”
Claim · model-written summary
The transgenes were hemizygous and outside chromosome two in both hybrids.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- F1-RecA and F1-NLS-RecA-LicBM3 hybridse0
- Outcome
- outside the second chromosome in the hemizygous statee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are contained in tomato F1-RecA and F1-NLS-RecA-LicBM3 hybrids outside the second chromosome in the hemizygous state”
Claim · model-written summary
Expression of E. coli recA increased recombination between some linked chromosome-two markers 1.2-1.5-fold.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- expression of the recA gene of E. colie0
- Gene
- recA gene of E. colie0
- Outcome
- frequency of recombination between some linked marker genes of the second chromosomee0
- Direction
- increasee0
- Value
- 1.2-1.5-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only the expression of the recA gene of E. coli in cells of the F1-RecA plants produced a 1.2-1.5-fold increase in the frequency of recombination between some linked marker genes of the second chromosome of tomato”
The same questions in other papers
transgenic · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.