Abstract
Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. Temperature strongly affected induction frequency of secondary embryogenesis. Relatively high temperature (30°C) and germinated SEs explants were effective for induction of secondary somatic embryos, and low temperature (20°C) was more suitable for further embryo development, plantlet conversion, and transplant survival. Somatic embryos formed on agar medium had larger cotyledons than those of embryos formed in liquid medium. Supplementing 0.1 mg L(-1) 6-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture and 36.5% in embryos from liquid culture. In vitro plants were successfully acclimatized in the greenhouse. The protocol established in this study will be helpful for large-scale vegetative propagation of this medicinal tree.
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Claims 10
Claim · model-written summary
Mature seed explants produced embryogenic callus on medium containing 2,4-dichlorophenoxyacetic acid.
- Taxon
- Hovenia dulcis
Hovenia dulcist0 - Organ / stage
- mature seed explantse0
- Treatment
- 2,4-dichlorophenoxyacetic acide0
- Outcome
- Embryogenic calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid.”
t0“Hovenia dulcis”
Claim · model-written summary
Primary somatic embryos developed only into abnormal plants.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Organ / stage
- Primary somatic embryose0
- Outcome
- abnormal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Primary somatic embryos (SEs) can only develop into abnormal plants.”
t0“Hovenia dulcis”
Claim · model-written summary
Secondary somatic embryogenesis yielded well-developed embryos in solid culture.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Outcome
- Well-developed SEse0
- Setting
- solide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures.”
t0“Hovenia dulcis”
Claim · model-written summary
Secondary somatic embryogenesis yielded well-developed embryos in liquid culture.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Outcome
- Well-developed SEse0
- Setting
- liquid culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures.”
t0“Hovenia dulcis”
Claim · model-written summary
A temperature of 30°C and germinated somatic embryo explants favored secondary embryo induction.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Organ / stage
- germinated SEs explantse0
- Treatment
- 30°Ce0
- Outcome
- induction of secondary somatic embryose0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relatively high temperature (30°C) and germinated SEs explants were effective for induction of secondary somatic embryos”
t0“Hovenia dulcis”
Claim · model-written summary
A temperature of 20°C was more suitable for embryo development, plantlet conversion and transplant survival.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Treatment
- 20°Ce0
- Outcome
- embryo development, plantlet conversion, and transplant survivale0
- Direction
- more suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low temperature (20°C) was more suitable for further embryo development, plantlet conversion, and transplant survival”
t0“Hovenia dulcis”
Claim · model-written summary
Somatic embryos formed on agar had larger cotyledons than those formed in liquid medium.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Outcome
- cotyledonse0
- Direction
- largere0
- Comparator
- liquid mediume0
- Setting
- agar mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos formed on agar medium had larger cotyledons than those of embryos formed in liquid medium.”
t0“Hovenia dulcis”
Claim · model-written summary
BA supplementation produced 43.3% plant conversion from solid-culture embryos.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Treatment
- 0.1 mg L(-1) 6-benzyladenine (BA)e0
- Outcome
- plant conversione0
- Direction
- effectivee0
- Value
- 43.3%e0
- Setting
- solid culturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supplementing 0.1 mg L(-1) 6-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture”
t0“Hovenia dulcis”
Claim · model-written summary
BA supplementation produced 36.5% plant conversion from liquid-culture embryos.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Treatment
- 0.1 mg L(-1) 6-benzyladenine (BA)e0
- Outcome
- plant conversione0
- Direction
- effectivee0
- Value
- 36.5%e0
- Setting
- liquid culturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supplementing 0.1 mg L(-1) 6-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture and 36.5% in embryos from liquid culture”
t0“Hovenia dulcis”
Claim · model-written summary
In vitro plants were successfully acclimatized in a greenhouse.
- Taxon
- Hovenia dulcisHovenia dulcist0
- Outcome
- acclimatizede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plants were successfully acclimatized in the greenhouse.”
t0“Hovenia dulcis”