Abstract
Acrylamide is produced in a wide variety of carbohydrate-rich foods during high-temperature cooking. Dietary acrylamide is a suspected human carcinogen, and health concerns related to dietary acrylamide have been raised worldwide. French fries and potato chips contribute a significant proportion to the average daily intake of acrylamide, especially in developed countries. One way to mitigate health concerns related to acrylamide is to develop potato cultivars that have reduced contents of the acrylamide precursors asparagine, glucose and fructose in tubers. We generated a large number of silencing lines of potato cultivar Russet Burbank by targeting the vacuolar invertase gene VInv and the asparagine synthetase genes StAS1 and StAS2 with a single RNA interference construct. The transcription levels of these three genes were correlated with reducing sugar (glucose and fructose) and asparagine content in tubers. Fried potato products from the best VInv/StAS1/StAS2-triple silencing lines contained only one-fifteenth of the acrylamide content of the controls. Interestingly, the extent of acrylamide reduction of the best triple silencing lines was similar to that of the best VInv-single silencing lines developed previously from the same potato cultivar Russet Burbank. These results show that an acrylamide mitigation strategy focused on developing potato cultivars with low reducing sugars is likely to be an effective and sufficient approach for minimizing the acrylamide-forming potential of French fry processing potatoes.
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Claims 4
Claim · model-written summary
Transcript levels of three genes correlated with reducing sugar and asparagine content in tubers.
- Taxon
- potato
Solanum tuberosume2 - Gene
- VInv and the asparagine synthetase genes StAS1 and StAS2e1
- Outcome
- reducing sugar (glucose and fructose) and asparagine content in tuberse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcription levels of these three genes were correlated with reducing sugar (glucose and fructose) and asparagine content in tubers.”
e1“the vacuolar invertase gene VInv and the asparagine synthetase genes StAS1 and StAS2”
e2“silencing lines of potato cultivar Russet Burbank”
Claim · model-written summary
Fried products from the best triple silencing lines had one-fifteenth of control acrylamide content.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- VInv/StAS1/StAS2-triple silencing linese0
- Outcome
- acrylamide contente0
- Direction
- onlye0
- Value
- one-fifteenthe0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fried potato products from the best VInv/StAS1/StAS2-triple silencing lines contained only one-fifteenth of the acrylamide content of the controls.”
Claim · model-written summary
The best triple and single silencing lines had similar acrylamide reduction.
- Taxon
- potatoSolanum tuberosume0
- Material
- triple silencing linese0
- Outcome
- extent of acrylamide reductione0
- Direction
- similare0
- Comparator
- VInv-single silencing linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the extent of acrylamide reduction of the best triple silencing lines was similar to that of the best VInv-single silencing lines developed previously from the same potato cultivar Russet Burbank.”
Claim · model-written summary
Developing low reducing sugar potato cultivars is likely sufficient to minimize acrylamide forming potential.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- developing potato cultivars with low reducing sugarse0
- Qualifier
- likely to be an effective and sufficient approache0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing potato cultivars with low reducing sugars is likely to be an effective and sufficient approach for minimizing the acrylamide-forming potential of French fry processing potatoes.”
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