Abstract
Suspension‐cultured carrot ( Daucus carota L. cv. Kintoki) cells were grown in calcium (Ca 2+ )‐deficient and normal liquid media. Cell growth was limited by the Ca 2+ deficiency. Similar amounts of pectic fractions were extracted from the walls of control and Ca 2+ ‐deprived cells, but the fractions from the walls of Ca 2+ ‐deprived cells showed a substantial decrease in galacturonic acid content. However, after 15 days of culture, Ca 2+ ‐deprived cells released galacturonic acid‐rich extracellular polysaccharides at twice the rate of control cells. The polysaccharides consisted of a mixture of several polymers containing predominantly arabinose, galactose and galacturonic acid. Ca 2+ ‐deprived cells also secreted three times more extracellular proteins, containing many glycan‐hydrolytic enzymes, into the medium than did normal cells. SDS‐PAGE analysis revealed several distinct changes in the polypeptide pattern in the medium of control and Ca 2+ ‐deprived cells. Activities of α ‐galactosidase, β ‐glucosidase and exo‐ polygalacturonase increased considerably during Ca 2+ deficiency, whereas α ‐ l ‐arabinofuranosidase and β ‐galactosidase activities were much reduced.
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Claims 9
Claim · model-written summary
Calcium deficiency limited carrot cell growth.
- Taxon
- carrot
Daucus carotae1 - Treatment
- Ca 2+ deficiencye0
- Outcome
- Cell growthe0
- Direction
- limitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell growth was limited by the Ca 2+ deficiency”
e1“carrot”
Claim · model-written summary
Control and calcium-deprived carrot cell walls yielded similar amounts of pectic fractions.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ ‐deprivede0
- Outcome
- pectic fractionse0
- Direction
- Similar amountse0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar amounts of pectic fractions were extracted from the walls of control and Ca 2+ ‐deprived cells”
e1“carrot”
Claim · model-written summary
Pectic fractions from calcium-deprived carrot cell walls had substantially less galacturonic acid.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ ‐deprivede0
- Outcome
- galacturonic acid contente0
- Direction
- substantial decreasee0
- Comparator
- controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fractions from the walls of Ca 2+ ‐deprived cells showed a substantial decrease in galacturonic acid content”
e1“carrot”
e2“walls of control and Ca 2+ ‐deprived cells”
Claim · model-written summary
After 15 days, calcium-deprived carrot cells released galacturonic-acid-rich extracellular polysaccharides at twice the control rate.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ ‐deprivede0
- Outcome
- galacturonic acid‐rich extracellular polysaccharidese0
- Direction
- twice the ratee0
- Value
- twice the ratee0
- Qualifier
- after 15 days of culturee0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after 15 days of culture, Ca 2+ ‐deprived cells released galacturonic acid‐rich extracellular polysaccharides at twice the rate of control cells”
e1“carrot”
Claim · model-written summary
The released polysaccharides were predominantly composed of arabinose, galactose and galacturonic acid.
- Taxon
- carrotDaucus carotae1
- Outcome
- polymers containing predominantly arabinose, galactose and galacturonic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polysaccharides consisted of a mixture of several polymers containing predominantly arabinose, galactose and galacturonic acid”
e1“carrot”
Claim · model-written summary
Calcium-deprived carrot cells secreted three times more extracellular proteins than normal cells.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ ‐deprivede0
- Outcome
- extracellular proteinse0
- Direction
- three times moree0
- Value
- three times moree0
- Comparator
- normal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca 2+ ‐deprived cells also secreted three times more extracellular proteins, containing many glycan‐hydrolytic enzymes, into the medium than did normal cells”
e1“carrot”
Claim · model-written summary
The medium polypeptide pattern differed between control and calcium-deprived carrot cells.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ ‐deprivede0
- Outcome
- polypeptide patterne0
- Direction
- distinct changese0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SDS‐PAGE analysis revealed several distinct changes in the polypeptide pattern in the medium of control and Ca 2+ ‐deprived cells”
e1“carrot”
Claim · model-written summary
Calcium deficiency considerably increased α-galactosidase, β-glucosidase and exo-polygalacturonase activities.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ deficiencye0
- Gene
- α ‐galactosidase, β ‐glucosidase and exo‐ polygalacturonasee0
- Outcome
- Activitiese0
- Direction
- increased considerablye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of α ‐galactosidase, β ‐glucosidase and exo‐ polygalacturonase increased considerably during Ca 2+ deficiency”
e1“carrot”
Claim · model-written summary
Calcium deficiency greatly reduced α-l-arabinofuranosidase and β-galactosidase activities.
- Taxon
- carrotDaucus carotae1
- Treatment
- Ca 2+ deficiencye2
- Gene
- α ‐ l ‐arabinofuranosidase and β ‐galactosidasee0
- Outcome
- activitiese0
- Direction
- much reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“α ‐ l ‐arabinofuranosidase and β ‐galactosidase activities were much reduced”
e1“carrot”
e2“Cell growth was limited by the Ca 2+ deficiency”
The same questions in other papers
calcium(2+) · growth · Daucus carota (carrot)Results agree
1 paper in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.