Abstract
Cotton (Gossypium hirsutum L.) fibers, one of the most important natural raw materials for textile industry, are highly elongated trichomes from epidermal cells of cotton ovules. DET2, an Arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (BR) biosynthesis. To understand the role of BRs in cotton fiber development, GhDET2, which putatively encodes a steroid 5alpha-reductase by sequence comparison, was cloned from developing fiber cells. In vitro assessment of GhDET2 protein activity confirmed that GhDET2 encodes a functional steroid 5alpha-redutase. High levels of GhDET2 transcript were detected during the fiber initiation stage and the fiber rapid elongation stage. Antisense-mediated suppression of GhDET2 inhibited both fiber initiation and fiber elongation. Similarly, treating cultured ovules with finasteride, a steroid 5alpha-reductase inhibitor, reduced fiber elongation. Inhibition of fiber cell elongation by expression of antisense GhDET2 or the finasteride treatment could be reversed by epibrassinolide, a biologically active BR. Furthermore, seed coat-specific expression of GhDET2 increased fiber number and length. Therefore, GhDET2 and BRs play a crucial role in the initiation and elongation of cotton fiber cells, suggesting that modulation of BR biosynthesis factors may improve fiber quality or yield.
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Claims 9
Claim · model-written summary
GhDET2 was cloned from developing fiber cells.
- Taxon
- Cotton
Gossypium hirsutumt0 - Organ / stage
- developing fiber cellse0
- Gene
- GhDET2e0
- Outcome
- clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhDET2, which putatively encodes a steroid 5alpha-reductase by sequence comparison, was cloned from developing fiber cells.”
t0“Cotton”
Claim · model-written summary
In vitro activity confirmed GhDET2 as a functional steroid 5alpha reductase.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhDET2e0
- Outcome
- functional steroid 5alpha-redutasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro assessment of GhDET2 protein activity confirmed that GhDET2 encodes a functional steroid 5alpha-redutase.”
t0“Cotton”
Claim · model-written summary
GhDET2 transcript levels were high during fiber initiation and rapid elongation.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- fiber initiation stage and the fiber rapid elongation stagee0
- Gene
- GhDET2e0
- Outcome
- GhDET2 transcripte0
- Direction
- High levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High levels of GhDET2 transcript were detected during the fiber initiation stage and the fiber rapid elongation stage.”
t0“Cotton”
Claim · model-written summary
Antisense suppression of GhDET2 inhibited fiber initiation and elongation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Antisense-mediated suppression of GhDET2e0
- Gene
- GhDET2e0
- Outcome
- fiber initiation and fiber elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense-mediated suppression of GhDET2 inhibited both fiber initiation and fiber elongation.”
t0“Cotton”
Claim · model-written summary
Finasteride treatment reduced fiber elongation in cultured ovules.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- cultured ovulese0
- Treatment
- finasteridee0
- Outcome
- fiber elongatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similarly, treating cultured ovules with finasteride, a steroid 5alpha-reductase inhibitor, reduced fiber elongation.”
t0“Cotton”
Claim · model-written summary
Epibrassinolide reversed fiber elongation inhibition from expression of antisense GhDET2.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- epibrassinolidee0
- Outcome
- Inhibition of fiber cell elongatione0
- Direction
- reversede0
- Comparator
- expression of antisense GhDET2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of fiber cell elongation by expression of antisense GhDET2 or the finasteride treatment could be reversed by epibrassinolide, a biologically active BR.”
t0“Cotton”
Claim · model-written summary
Epibrassinolide reversed fiber elongation inhibition from the finasteride treatment.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- epibrassinolidee0
- Outcome
- Inhibition of fiber cell elongatione0
- Direction
- reversede0
- Comparator
- the finasteride treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of fiber cell elongation by expression of antisense GhDET2 or the finasteride treatment could be reversed by epibrassinolide, a biologically active BR.”
t0“Cotton”
Claim · model-written summary
Seed coat specific GhDET2 expression increased fiber number and length.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- seed coat-specific expression of GhDET2e0
- Gene
- GhDET2e0
- Outcome
- fiber number and lengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, seed coat-specific expression of GhDET2 increased fiber number and length.”
t0“Cotton”
Claim · model-written summary
The authors suggest modulating BR biosynthesis factors to improve cotton fiber quality or yield.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhDET2e0
- Outcome
- modulation of BR biosynthesis factors may improve fiber quality or yielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhDET2 and BRs play a crucial role in the initiation and elongation of cotton fiber cells, suggesting that modulation of BR biosynthesis factors may improve fiber quality or yield.”
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See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.