Abstract
ABSTRACT The bacterial gene mtlD , which encodes mannitol 1‐phosphate dehydrogenase (E. C. 1. 1. 1. 17), was transformed into Arabidopsis thaliana and expressed under control of the CaMV 35S promoter. MtlD ‐transformants accumulated mannitol, a sugar alcohol that is not normally found in Arabidopsis . Amounts of soluble carbohydrates, sucrose, glucose, fructose, myo‐inositol and mannitol were determined in different tissues of wild‐type and transgenic plants. We estimated that less than 1& of the carbon assimilated was converted into mannitol by the transgenic plants. The establishment of individual transformed lines (after self‐crossing three times) resulted in high and low mannitol‐producing lines which were stably maintained. The presence of mannitol did not alter plant appearance or growth habit. When MtlD‐expressing seeds and control seeds (T3 generation) were imbibed with solutions containing NaCl (range 0 to 400 mol m −3 ), transgenic seeds containing mannitol germinated in medium supplemented with up to 400 mol m −3 NaCl, while control seeds ceased germination at 100 mol m −3 NaCl. It is doubtful whether the ability to germinate in high salt was a result of an osmotic effect exerted by elevated levels of mannitol, considering that mannitol concentrations were in the mol m −3 range in seeds. A specific effect of polyols, for example on the integrity of subcellular membranes or enzymes, cannot be excluded.
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Claims 6
Claim · model-written summary
mtlD transformants accumulated mannitol absent from normal Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- MtlD ‐transformantse0
- Treatment
- MtlD ‐transformantse0
- Outcome
- accumulated mannitole0
- Direction
- accumulatede0
- Comparator
- Arabidopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MtlD ‐transformants accumulated mannitol, a sugar alcohol that is not normally found in Arabidopsis .”
Claim · model-written summary
Less than 1& of assimilated carbon was converted to mannitol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- carbon assimilated was converted into mannitole0
- Value
- less than 1&e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“less than 1& of the carbon assimilated was converted into mannitol by the transgenic plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Self crossing established stable high and low mannitol producing lines.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- high and low mannitol‐producing linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high and low mannitol‐producing lines which were stably maintained.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Mannitol presence did not change plant appearance or growth habit.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Compound
- mannitole0
- Outcome
- plant appearance or growth habite0
- Direction
- did not altere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of mannitol did not alter plant appearance or growth habit.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Mannitol containing transgenic seeds germinated up to 400 mol m −3 NaCl.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- up to 400 mol m −3 NaCle0
- Outcome
- germinatede0
- Value
- 400 mol m −3e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic seeds containing mannitol germinated in medium supplemented with up to 400 mol m −3 NaCl”
t0“Arabidopsis thaliana”
Claim · model-written summary
Control seeds ceased germination at 100 mol m −3 NaCl.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- 100 mol m −3 NaCle0
- Outcome
- ceased germinatione0
- Value
- 100 mol m −3e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“control seeds ceased germination at 100 mol m −3 NaCl.”
t0“Arabidopsis thaliana”
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