Abstract
The effects of different culture temperatures, culture media and saccharides on the anther culture efficiency in two salt tolerant rice (Oryza sativa ssp. indica) cultivars, Pokkali and Nona Bokra, were examined. Comparison of two culture temperatures (constant temperature at 25°C and alternating temperatures at 30°C/20°C) indicated that alternating temperatures showed remarkable effects not only on the callus induction but also on the plant regeneration. Among the basal media examined, higher callus productivity was observed in SK-I medium and higher shoot productivity was obtained in SK-II medium than other basal media. When alternating temperatures and revised N6 medium including maltose were used throughout anther culture, callus productivity and green shoot productivity showed the highest score in the salt tolerant two cultivars used in this study. Maltose and alternating temperatures increases the anther culture efficiency of recalcitrant indica rice cultivars. This is the first report suggesting high production of haploids or doubled haploids from anther culture of these salt tolerant indica cultivars.
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Claims 7
Claim · model-written summary
Alternating temperatures improved callus induction relative to constant temperature.
- Taxon
- rice
Oryza sativae1 - Treatment
- alternating temperatures at 30°C/20°Ce0
- Outcome
- callus inductione0
- Comparator
- constant temperature at 25°Ce0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constant temperature at 25°C and alternating temperatures at 30°C/20°C) indicated that alternating temperatures showed remarkable effects not only on the callus induction but also on the plant regeneration”
e1“rice (Oryza sativa ssp. indica) cultivars”
Claim · model-written summary
Alternating temperatures affected plant regeneration relative to constant temperature.
- Taxon
- riceOryza sativae1
- Treatment
- alternating temperatures at 30°C/20°Ce0
- Outcome
- plant regeneratione0
- Comparator
- constant temperature at 25°Ce0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constant temperature at 25°C and alternating temperatures at 30°C/20°C) indicated that alternating temperatures showed remarkable effects not only on the callus induction but also on the plant regeneration”
e1“rice (Oryza sativa ssp. indica) cultivars”
Claim · model-written summary
SK-I medium produced more callus than other basal media.
- Taxon
- riceOryza sativae1
- Treatment
- SK-I mediume0
- Outcome
- callus productivitye0
- Direction
- highere0
- Comparator
- other basal mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher callus productivity was observed in SK-I medium and higher shoot productivity was obtained in SK-II medium than other basal media”
e1“rice (Oryza sativa ssp. indica) cultivars”
Claim · model-written summary
SK-II medium produced more shoots than other basal media.
- Taxon
- riceOryza sativae1
- Treatment
- SK-II mediume0
- Outcome
- shoot productivitye0
- Direction
- highere0
- Comparator
- other basal mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher shoot productivity was obtained in SK-II medium than other basal media”
e1“rice (Oryza sativa ssp. indica) cultivars”
Claim · model-written summary
Alternating temperatures and maltose-containing revised N6 medium gave the highest callus productivity in both cultivars.
- Taxon
- riceOryza sativae1
- Material
- Pokkali and Nona Bokrae1
- Treatment
- alternating temperatures and revised N6 medium including maltosee0
- Outcome
- callus productivitye0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When alternating temperatures and revised N6 medium including maltose were used throughout anther culture, callus productivity and green shoot productivity showed the highest score in the salt tolerant two cultivars used in this study”
e1“rice (Oryza sativa ssp. indica) cultivars, Pokkali and Nona Bokra”
Claim · model-written summary
Alternating temperatures and maltose-containing revised N6 medium gave the highest green shoot productivity in both cultivars.
- Taxon
- riceOryza sativae1
- Material
- Pokkali and Nona Bokrae1
- Treatment
- alternating temperatures and revised N6 medium including maltosee0
- Outcome
- green shoot productivitye0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When alternating temperatures and revised N6 medium including maltose were used throughout anther culture, callus productivity and green shoot productivity showed the highest score in the salt tolerant two cultivars used in this study”
e1“rice (Oryza sativa ssp. indica) cultivars, Pokkali and Nona Bokra”
Claim · model-written summary
The anther culture protocol was reported to enable high production of haploids or doubled haploids.
- Taxon
- riceOryza sativae1
- Outcome
- production of haploids or doubled haploidse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is the first report suggesting high production of haploids or doubled haploids from anther culture of these salt tolerant indica cultivars”
e1“rice (Oryza sativa ssp. indica) cultivars”
The same questions in other papers
maltose · yield · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
in vitro · yield · Oryza sativa (rice)Positive and negative results
50 papers in the same direction as this paper · 7 in the opposite direction · 2 reporting no difference from an alternative
- negative
- negative
- negative
56 papers more in this group
See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.