Abstract
Indica rice is reported to be recalcitrant to in-vitro techniques including anther culture. Pokkali and Nona Bokra, indica rice cultivars, have been included in rice hybridization programs to develop salt tolerant cultivars but are recalcitrant to anther culture technique. To improve the anther culture efficiency of these cultivars, the effectiveness of culture temperatures and sacchrides on callus induction (CI) and plant regeneration (PR) were investigated. Callus productivity (CP) and green shoot productivity (GSP) were higher in SK media under alternate temperatures (20C, 10 hours/30C, 14 hours). Pokkali was more responsive than Nona Bokra. At CI stage, responding anthers (RA) and CP were increased 1.7 and 2.7 times in Pokkali, respectively, in SK-I medium and alternate temperatures. At PR stage, regeneration of green shoot (GS) and GSP were increased 3.1 and 8.6 times, respectively, in SK-II medium under alternate temperatures. The effects of saccharides (sucrose and maltose) were investigated further in revised N6 media. Maltose media enhanced RA 1.4 and 1.1 times, and CP 1.8 and 1.5 times in Nona Bokra and Pokkali, respectively. Whereas, GSP was increased 1.8 and 3.1 times in Nona Bokra and Pokkali, respectively, in the regeneration medium included maltose. These results indicated the usefulness of alternate culture temperatures and maltose in improving the anther culture efficiency of Pokkali and Nona Bokra. Hence, maltose and alternate culture temperatures could be beneficial factors to develop an appropriate anther culture protocol for other recalcitrant indica rice cultivars.
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Claims 13
Claim · model-written summary
Callus productivity and green shoot productivity were higher in SK media under alternate temperatures.
- Taxon
- indica rice cultivars
Oryza sativa Indica Groupt0 - Treatment
- SK media under alternate temperatures (20C, 10 hours/30C, 14 hours)e0
- Outcome
- Callus productivity (CP) and green shoot productivity (GSP)e0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus productivity (CP) and green shoot productivity (GSP) were higher in SK media under alternate temperatures (20C, 10 hours/30C, 14 hours).”
t0“indica rice cultivars”
Claim · model-written summary
Pokkali was more responsive to anther culture than Nona Bokra.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie0
- Outcome
- more responsivee0
- Direction
- more responsivee0
- Comparator
- Nona Bokrae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pokkali was more responsive than Nona Bokra.”
t0“indica rice cultivars”
Claim · model-written summary
In Pokkali, responding anthers increased 1.7 times under SK-I medium and alternate temperatures.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- SK-I medium and alternate temperaturese0
- Outcome
- responding anthers (RA)e0
- Direction
- increasede0
- Value
- 1.7e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At CI stage, responding anthers (RA) and CP were increased 1.7 and 2.7 times in Pokkali, respectively, in SK-I medium and alternate temperatures.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
In Pokkali, CP increased 2.7 times under SK-I medium and alternate temperatures.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- SK-I medium and alternate temperaturese0
- Outcome
- CPe0
- Direction
- increasede0
- Value
- 2.7 timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At CI stage, responding anthers (RA) and CP were increased 1.7 and 2.7 times in Pokkali, respectively, in SK-I medium and alternate temperatures.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
In Pokkali, regeneration of green shoot (GS) increased 3.1 times in SK-II medium under alternate temperatures.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- SK-II medium under alternate temperaturese0
- Outcome
- regeneration of green shoot (GS)e0
- Direction
- increasede0
- Value
- 3.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At PR stage, regeneration of green shoot (GS) and GSP were increased 3.1 and 8.6 times, respectively, in SK-II medium under alternate temperatures.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
In Pokkali, GSP increased 8.6 times in SK-II medium under alternate temperatures.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- SK-II medium under alternate temperaturese0
- Outcome
- GSPe0
- Direction
- increasede0
- Value
- 8.6e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At PR stage, regeneration of green shoot (GS) and GSP were increased 3.1 and 8.6 times, respectively, in SK-II medium under alternate temperatures.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose medium enhanced RA 1.4 times in Nona Bokra.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Nona Bokrae1
- Treatment
- Maltose mediae0
- Outcome
- RAe0
- Value
- 1.4e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose media enhanced RA 1.4 and 1.1 times, and CP 1.8 and 1.5 times in Nona Bokra and Pokkali, respectively.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose medium enhanced RA 1.1 times in Pokkali.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- Maltose mediae0
- Outcome
- RAe0
- Value
- 1.1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose media enhanced RA 1.4 and 1.1 times, and CP 1.8 and 1.5 times in Nona Bokra and Pokkali, respectively.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose medium enhanced CP 1.8 times in Nona Bokra.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Nona Bokrae1
- Treatment
- Maltose mediae0
- Outcome
- CPe0
- Value
- 1.8e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose media enhanced RA 1.4 and 1.1 times, and CP 1.8 and 1.5 times in Nona Bokra and Pokkali, respectively.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose medium enhanced CP 1.5 times in Pokkali.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- Maltose mediae0
- Outcome
- CPe0
- Value
- 1.5e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose media enhanced RA 1.4 and 1.1 times, and CP 1.8 and 1.5 times in Nona Bokra and Pokkali, respectively.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose regeneration medium increased green shoot productivity 1.8 times in Nona Bokra.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Nona Bokrae1
- Treatment
- regeneration medium included maltosee0
- Outcome
- GSPe0
- Direction
- increasede0
- Value
- 1.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GSP was increased 1.8 and 3.1 times in Nona Bokra and Pokkali, respectively, in the regeneration medium included maltose.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
Maltose regeneration medium increased green shoot productivity 3.1 times in Pokkali.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupt0
- Material
- Pokkalie1
- Treatment
- regeneration medium included maltosee0
- Outcome
- GSPe0
- Direction
- increasede0
- Value
- 3.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GSP was increased 1.8 and 3.1 times in Nona Bokra and Pokkali, respectively, in the regeneration medium included maltose.”
t0“indica rice cultivars”
e1“Pokkali and Nona Bokra, indica rice cultivars”
Claim · model-written summary
The authors propose maltose and alternate temperatures as useful for anther culture of recalcitrant indica rice.
- Taxon
- indica rice cultivarsOryza sativa Indica Groupe0
- Treatment
- maltose and alternate culture temperaturese0
- Outcome
- beneficial factors to develop an appropriate anther culture protocole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maltose and alternate culture temperatures could be beneficial factors to develop an appropriate anther culture protocol for other recalcitrant indica rice cultivars.”
The same questions in other papers
maltose · yield · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
in vitro · yield · Oryza sativa (rice)Positive and negative results
50 papers in the same direction as this paper · 7 in the opposite direction · 2 reporting no difference from an alternative
- negative
- negative
- negative
56 papers more in this group
See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.