Abstract
A full-length genomic cDNA clone of a plum pox potyvirus (PPV) isolate belonging to the M strain (PPV-PS) has been cloned downstream from a bacteriophage T7 polymerase promoter and sequenced. Transcripts from the resulting plasmid, pGPPVPS, were infectious and, in herbaceous hosts, produced symptoms that differed from those of virus progeny of pGPPV, a full-length genomic cDNA clone of the D strain PPV-R. Viable PPV-R/-PS chimeric viruses were constructed by recombination of the cDNA clones in vitro. Analysis of plants infected with the different chimeras indicated that sequences encoding the most variable regions of the potyvirus genome, the P1 and capsid protein coding sequences, were not responsible for symptom differences between the two PPV isolates in herbaceous hosts. On the contrary, complex symptomatology determinants seem to be located in the central region of the PPV genome. The results indicate that a genomic fragment that encodes 173 aa from the C-terminal part of the P3+6K(1) coding region is enough to confer, on a PPV-R background, a PS phenotype in Nicotiana clevelandii. This pathogenicity determinant also participates in symptom induction in Pisum sativum, although the region defining the PS phenotype in this host is probably restricted to 74 aa.
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Claims 5
Claim · model-written summary
The PPV-PS clone produced symptoms in herbaceous hosts different from those caused by the PPV-R clone.
Not reportedTaxon
Verbatim
e0“Transcripts from the resulting plasmid, pGPPVPS, were infectious and, in herbaceous hosts, produced symptoms that differed from those of virus progeny of pGPPV, a full-length genomic cDNA clone of the D strain PPV-R.”
Claim · model-written summary
P1 and capsid protein coding sequences did not account for the symptom differences in herbaceous hosts.
Not reportedTaxonDirection
Verbatim
e0“the P1 and capsid protein coding sequences, were not responsible for symptom differences between the two PPV isolates in herbaceous hosts.”
Claim · model-written summary
The central PPV genome region appears to contain complex symptom determinants.
- Interactor
- PPVe0pathogen
- Outcome
- complex symptomatology determinantse0
- Qualifier
- central region of the PPV genomee0
Not reportedTaxon
Verbatim
e0“complex symptomatology determinants seem to be located in the central region of the PPV genome.”
Claim · model-written summary
A 173 aa P3+6K(1) fragment conferred a PS phenotype on a PPV-R background in Nicotiana clevelandii.
- Taxon
- Nicotiana clevelandii
Nicotiana clevelandiie0 - Interactor
- PPV-Re0pathogen
- Gene
- P3+6K(1)e0
- Outcome
- PS phenotypee0
- Qualifier
- 173 aae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a genomic fragment that encodes 173 aa from the C-terminal part of the P3+6K(1) coding region is enough to confer, on a PPV-R background, a PS phenotype in Nicotiana clevelandii.”
Claim · model-written summary
The determinant also contributed to symptoms in Pisum sativum, with the defining region probably restricted to 74 aa.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Outcome
- symptom inductione0
- Qualifier
- probably restricted to 74 aae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This pathogenicity determinant also participates in symptom induction in Pisum sativum, although the region defining the PS phenotype in this host is probably restricted to 74 aa.”