Abstract
The multiplication and the distribution of plant material is one of the objectives of plant tissue culture laboratory of African Research Centre on Bananas and Plantains (ARCBAP). Some banana (Musa spp.) cultivars including Topala, Fougamou, Gros-Michel shows low proliferation when cultured in vitro. Other cultivars like Dwarf-Kalapua, Pelipita and Kalapua 2 take long time (two months) to be differentiated in in vitro multiplication. All these cultivars have interested agronomic characteristics and have partial or total resistance to Black Leaf Streak Disease (BLSD). In the aim of increasing proliferation rate, shoot tips of those cultivars were cultured on Murashige and Skoog nutrient salts supplemented with different concentrations of thidiazuron (0.05-2 µM) in comparison with the standard medium containing 2 mg/l of BAP. Growth parameters (length and diameter of the stem) and proliferation parameters (number of shoots, leaves and roots formed) were measured six weeks after introduction of explants on the medium during introduction and proliferation phases. Results showed that proliferation was better whichever the cultivar with low thidiazuron concentration (0.05 to 0.8 µM). The plant growth in treatments did not show any significant difference as compared to the control, however, plant development was ameliorated in all cultivars at thidiazuron concentration of 0.05-0.4 µM. Positive correlations were observed between shoot proliferation and leaf or root formation in Fougamou, Gros-Michel and Pelipita cultivars, except for cultivar Topala.
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Claims 8
Claim · model-written summary
Topala, Fougamou and Gros-Michel had low proliferation in vitro.
Verbatim
e0“Topala, Fougamou, Gros-Michel shows low proliferation when cultured in vitro”
t0“Banana”
Claim · model-written summary
Dwarf-Kalapua, Pelipita and Kalapua 2 took two months to differentiate.
Verbatim
e0“Dwarf-Kalapua, Pelipita and Kalapua 2 take long time (two months) to be differentiated in in vitro multiplication”
t0“Banana”
Claim · model-written summary
The cultivars had partial or total resistance to BLSD.
Verbatim
e0“All these cultivars have interested agronomic characteristics and have partial or total resistance to Black Leaf Streak Disease (BLSD)”
t0“Banana”
Claim · model-written summary
Low thidiazuron concentrations improved proliferation across cultivars.
- Taxon
- bananat0
- Treatment
- low thidiazuron concentration (0.05 to 0.8 µM)e0
- Outcome
- proliferatione0
- Direction
- bettere0
Not reportedComparator
Verbatim
e0“proliferation was better whichever the cultivar with low thidiazuron concentration (0.05 to 0.8 µM)”
t0“Banana”
Claim · model-written summary
Growth did not significantly differ from control.
Verbatim
e0“The plant growth in treatments did not show any significant difference as compared to the control”
t0“Banana”
Claim · model-written summary
Thidiazuron at 0.05-0.4 µM improved development in all cultivars.
- Taxon
- bananat0
- Treatment
- thidiazuron concentration of 0.05-0.4 µMe0
- Outcome
- plant developmente0
- Direction
- amelioratede0
Not reportedComparator
Verbatim
e0“plant development was ameliorated in all cultivars at thidiazuron concentration of 0.05-0.4 µM”
t0“Banana”
Claim · model-written summary
Shoot proliferation correlated positively with leaf and root formation in Fougamou, Gros-Michel and Pelipita.
Verbatim
e0“Positive correlations were observed between shoot proliferation and leaf or root formation in Fougamou, Gros-Michel and Pelipita cultivars”
t0“Banana”
Claim · model-written summary
Topala was an exception to the positive correlations.
Verbatim
e0“except for cultivar Topala”
t0“Banana”
The same questions in other papers
Thidiazuron · growth · MusaResults differ
19 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- positive
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.