Abstract
The effects of various factors in culture medium on the phenolics production from cultured tobacco cells (free and immobilized) were studied. It was found that removing the growth hormone from the medium increased the productivities of phenolics for both free and immobilized cultures. Low initial sucrose concentration in the medium restricted growth and resulted in high cellular productivities of the phenolics for freely suspended cells, but not for the immobilized cells. Addition of 1.4% DMSO to standard culture medium greatly increased phenolics productivities without affecting cell viability in both free and immobilized cell cultures. Continuous operation of a packed-column reactor of the immobilized cells was achieved for 500 hours. Aeration was accomplished by diffusing pure oxygen through silicone tubing placed inside the reactor. It was found that prolonged cell viability was contingent upon initially operating the reactor with total recycle for several days, and then introducing fresh feed while maintaining a high recycle rate. Immobilized cells packed in a continuous column reactor achieved productivities more than twice that achieved in any batch run.
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Claims 9
Claim · model-written summary
Removing growth hormone increased phenolics productivity in free and immobilized tobacco cells.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- removing the growth hormonee0
- Outcome
- productivities of phenolicse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removing the growth hormone from the medium increased the productivities of phenolics for both free and immobilized cultures”
t0“tobacco cell cultures”
Claim · model-written summary
Low initial sucrose restricted growth of freely suspended tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- freely suspended cellse0
- Treatment
- Low initial sucrose concentratione0
- Outcome
- growthe0
- Direction
- restrictede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low initial sucrose concentration in the medium restricted growth and resulted in high cellular productivities of the phenolics for freely suspended cells, but not for the immobilized cells”
t0“tobacco cell cultures”
Claim · model-written summary
Low initial sucrose produced high cellular phenolics productivity in freely suspended tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- freely suspended cellse0
- Treatment
- Low initial sucrose concentratione0
- Outcome
- cellular productivities of the phenolicse0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low initial sucrose concentration in the medium restricted growth and resulted in high cellular productivities of the phenolics for freely suspended cells, but not for the immobilized cells”
t0“tobacco cell cultures”
Claim · model-written summary
Low initial sucrose did not produce high cellular phenolics productivity in immobilized tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- immobilized cellse0
- Treatment
- Low initial sucrose concentratione0
- Outcome
- cellular productivities of the phenolicse0
- Direction
- note0
- Comparator
- freely suspended cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low initial sucrose concentration in the medium restricted growth and resulted in high cellular productivities of the phenolics for freely suspended cells, but not for the immobilized cells”
t0“tobacco cell cultures”
Claim · model-written summary
Adding 1.4% DMSO greatly increased phenolics productivity in free and immobilized tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Addition of 1.4% DMSOe0
- Outcome
- phenolics productivitiese0
- Direction
- greatly increasede0
- Comparator
- standard culture mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 1.4% DMSO to standard culture medium greatly increased phenolics productivities without affecting cell viability in both free and immobilized cell cultures”
t0“tobacco cell cultures”
Claim · model-written summary
Adding 1.4% DMSO did not affect viability of free and immobilized tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Addition of 1.4% DMSOe0
- Outcome
- cell viabilitye0
- Direction
- without affectinge0
- Comparator
- standard culture mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 1.4% DMSO to standard culture medium greatly increased phenolics productivities without affecting cell viability in both free and immobilized cell cultures”
t0“tobacco cell cultures”
Claim · model-written summary
Continuous operation of the immobilized tobacco cell reactor was achieved for 500 hours.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- Continuous operatione0
- Value
- 500 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Continuous operation of a packed-column reactor of the immobilized cells was achieved for 500 hours”
t0“tobacco cell cultures”
Claim · model-written summary
Prolonged tobacco cell viability depended on initial total recycle followed by fresh feed with high recycle.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- initially operating the reactor with total recycle for several days, and then introducing fresh feed while maintaining a high recycle ratee0
- Outcome
- prolonged cell viabilitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prolonged cell viability was contingent upon initially operating the reactor with total recycle for several days, and then introducing fresh feed while maintaining a high recycle rate”
t0“tobacco cell cultures”
Claim · model-written summary
Immobilized tobacco cells in a continuous column reactor achieved more than twice the phenolics productivity of any batch run.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- continuous column reactore0
- Outcome
- productivitiese0
- Direction
- more than twicee0
- Value
- more than twicee0
- Comparator
- any batch rune0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immobilized cells packed in a continuous column reactor achieved productivities more than twice that achieved in any batch run”
t0“tobacco cell cultures”
The same questions in other papers
dimethyl sulfoxide · quality: phytochemicals · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- no effect
sucrose · growth · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →dimethyl sulfoxide · cytology · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.