Abstract
Data are presented which indicate that dimethylsulfoxide (DMSO) acts selectively on the plasma membrane of cultured tobacco cells, rendering it more permeable to small molecules, while having a far smaller effect on the permeability of the vacuolar membrane. The results which support this conclusion are: (a) DMSO (5 to 10%, by volume) causes complete release of [(14)C]tryptophan newly synthesized from [(14)C]indole while causing efflux of only about 20% of the total intracellular tryptophan pool; (b) similar concentrations of DMSO do not cause substantial release from these cells of phenolic compounds or preloaded neutral red, nor of beta-cyanin from fresh beet discs; (c) kinetic studies of release of tryptophan and neutral sugars and of efflux of (86)Rb(+) show that DMSO selectively promotes rapid release of a portion of the total pool, followed by a substantially slower release of the remaining pool; (d) when tobacco cell protoplasts are incubated in the presence of 7.5% (by volume) DMSO, rapid lysis is observed concomitant with the release of intact vacuoles. These data indicate that a procedure involving a brief treatment of intact plant cells or tissues with DMSO may be used to assess the distribution of metabolites between cytoplasmic and vacuolar compartments.
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Claims 8
Claim · model-written summary
DMSO acts selectively on the plasma membrane of cultured tobacco cells, making it more permeable to small molecules, with a far smaller effect on the vacuolar membrane.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- plasma membranee0
- Treatment
- dimethylsulfoxide (DMSO)e0
- Outcome
- permeabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dimethylsulfoxide (DMSO) acts selectively on the plasma membrane of cultured tobacco cells, rendering it more permeable to small molecules, while having a far smaller effect on the permeability of the vacuolar membrane”
Claim · model-written summary
DMSO (5 to 10%) caused complete release of newly synthesized [14C]tryptophan from tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- DMSO (5 to 10%, by volume)e1
- Outcome
- release of [(14)C]tryptophan newly synthesized from [(14)C]indolee1
- Direction
- completee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cultured tobacco cells”
e1“DMSO (5 to 10%, by volume) causes complete release of [(14)C]tryptophan newly synthesized from [(14)C]indole”
Claim · model-written summary
DMSO caused efflux of only about 20% of the total intracellular tryptophan pool.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- total intracellular tryptophan poole1
- Direction
- effluxe1
- Value
- about 20%e1
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cultured tobacco cells”
e1“while causing efflux of only about 20% of the total intracellular tryptophan pool”
Claim · model-written summary
Similar DMSO concentrations did not cause substantial release of phenolic compounds or preloaded neutral red from the tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- DMSOe1
- Outcome
- release from these cells of phenolic compounds or preloaded neutral rede1
- Direction
- not cause substantial releasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cultured tobacco cells”
e1“similar concentrations of DMSO do not cause substantial release from these cells of phenolic compounds or preloaded neutral red”
Claim · model-written summary
DMSO did not cause substantial release of beta-cyanin from fresh beet discs.
- Taxon
- beet
Beta vulgarise1 - Treatment
- DMSOe1
- Outcome
- releasee1
- Direction
- nor of beta-cyanine1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“similar concentrations of DMSO do not cause substantial release from these cells of phenolic compounds or preloaded neutral red, nor of beta-cyanin from fresh beet discs”
Claim · model-written summary
Kinetic studies showed DMSO selectively promotes rapid release of a portion of the total pool of tryptophan, neutral sugars and (86)Rb(+), followed by much slower release of the remainder.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- DMSOe1
- Outcome
- release of tryptophan and neutral sugars and of efflux of (86)Rb(+)e1
- Direction
- rapid releasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cultured tobacco cells”
e1“kinetic studies of release of tryptophan and neutral sugars and of efflux of (86)Rb(+) show that DMSO selectively promotes rapid release of a portion of the total pool, followed by a substantially slower release of the remaining pool”
Claim · model-written summary
Tobacco cell protoplasts incubated with 7.5% (by volume) DMSO showed rapid lysis concomitant with release of intact vacuoles.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- 7.5% (by volume) DMSOe1
- Outcome
- rapid lysise1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“when tobacco cell protoplasts are incubated in the presence of 7.5% (by volume) DMSO, rapid lysis is observed concomitant with the release of intact vacuoles”
Claim · model-written summary
A brief DMSO treatment of intact plant cells or tissues may be used to assess the distribution of metabolites between cytoplasmic and vacuolar compartments.
Verbatim
e1“a procedure involving a brief treatment of intact plant cells or tissues with DMSO may be used to assess the distribution of metabolites between cytoplasmic and vacuolar compartments”
The same questions in other papers
dimethyl sulfoxide · quality: phytochemicals · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.