Abstract
Abstract The mutagenic effects of gamma radiation and sodium azide (SA) immersion of explants on culturability and frequency of somaclonal variation in indica rice were investigated. The results showed that young inflorescence and mature embryo were satisfactory explants both for in vitro culture and for mutagenesis. 1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration, 0.5—1 kR would be the appropriate exposure for young inflorescence to produce a better culture response and higher variability, and 2.5—5.0 kR the optimal dose range for mature embryos. Immersion of mature seed in 2—4 mM sodium azide solution is a concentration level to induce these effects in indica rice. Application of optimal gamma exposure and chemical mutagen to rice explants enhanced the somaclonal variation frequency and provided a large number of variants useful for rice breeding.
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Claims 8
Claim · model-written summary
1 kR gamma rays applied to immature embryos of indica rice led to a higher rate of callusing and plantlet regeneration.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Organ / stage
- immature embryose1
- Treatment
- 1 kR gamma rayse1
- Outcome
- rate of callusing and plantlet regeneratione1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“somaclonal variation in indica rice”
e1“1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration”
Claim · model-written summary
Exposure of calli from mature seeds to 250—500 R gamma rays led to a higher rate of callusing and plantlet regeneration in indica rice.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Organ / stage
- calli derived from mature seedse1
- Treatment
- 250—500 Re1
- Outcome
- rate of callusing and plantlet regeneratione1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“somaclonal variation in indica rice”
e1“1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration”
Claim · model-written summary
0.5—1 kR is the appropriate gamma exposure for young inflorescence of indica rice to give a better culture response and higher variability.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Organ / stage
- young inflorescencee2
- Outcome
- culture responsee2
- Value
- 0.5—1 kRe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“somaclonal variation in indica rice”
e2“0.5—1 kR would be the appropriate exposure for young inflorescence to produce a better culture response and higher variability”
Claim · model-written summary
2.5—5.0 kR is the optimal gamma dose range for mature embryos of indica rice.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Organ / stage
- mature embryose3
- Outcome
- optimal dose rangee3
- Value
- 2.5—5.0 kRe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“somaclonal variation in indica rice”
e3“2.5—5.0 kR the optimal dose range for mature embryos”
Claim · model-written summary
Immersion of mature seed in 2—4 mM sodium azide is a concentration level that induces these effects in indica rice.
- Taxon
- indica rice
Oryza sativa Indica Groupe4 - Organ / stage
- mature seede4
- Treatment
- sodium azidee4
- Outcome
- concentration level to induce these effectse4
- Value
- 2—4 mMe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e4“Immersion of mature seed in 2—4 mM sodium azide solution is a concentration level to induce these effects in indica rice”
Claim · model-written summary
Optimal gamma exposure combined with a chemical mutagen enhanced the somaclonal variation frequency in rice explants.
- Taxon
- rice
Oryza sativae5
- Treatment
- optimal gamma exposure and chemical mutagene5
- Outcome
- somaclonal variation frequencye5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Application of optimal gamma exposure and chemical mutagen to rice explants enhanced the somaclonal variation frequency”
Claim · model-written summary
Young inflorescence and mature embryo of indica rice were satisfactory explants for in vitro culture and mutagenesis.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Organ / stage
- young inflorescence and mature embryoe6
- Outcome
- satisfactory explantse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“somaclonal variation in indica rice”
e6“The results showed that young inflorescence and mature embryo were satisfactory explants both for in vitro culture and for mutagenesis”
Claim · model-written summary
The mutagenesis and culture procedure provided a large number of rice variants useful for breeding.
- Taxon
- rice
Oryza sativae7
- Outcome
- variants useful for rice breedinge7
- Value
- a large numbere7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“provided a large number of variants useful for rice breeding”
The same questions in other papers
radiation · growth · Oryza sativa (rice)Results differ
21 papers in the same direction as this paper · 60 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- negative
80 papers more in this group
See all 84 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.