Paper · 1992 · Plant Breeding

In Vitro Culture of Hybrid Indica Rice Combined with Mutagenesis

M. W. Gao; Q. H. Cai; Z. Q. Liang

8
claims
13
verbatim quotes
10
citations
3
research areas
—
Not open access

Abstract

Abstract The mutagenic effects of gamma radiation and sodium azide (SA) immersion of explants on culturability and frequency of somaclonal variation in indica rice were investigated. The results showed that young inflorescence and mature embryo were satisfactory explants both for in vitro culture and for mutagenesis. 1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration, 0.5—1 kR would be the appropriate exposure for young inflorescence to produce a better culture response and higher variability, and 2.5—5.0 kR the optimal dose range for mature embryos. Immersion of mature seed in 2—4 mM sodium azide solution is a concentration level to induce these effects in indica rice. Application of optimal gamma exposure and chemical mutagen to rice explants enhanced the somaclonal variation frequency and provided a large number of variants useful for rice breeding.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

1 kR gamma rays applied to immature embryos of indica rice led to a higher rate of callusing and plantlet regeneration.

Organ / stage
immature embryose1
Treatment
1 kR gamma rayse1
Outcome
rate of callusing and plantlet regeneratione1
Direction
highere1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“somaclonal variation in indica rice”

✓ located verbatim in the source · abstract, characters 128–163 · show in text ↑
e1

“1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration”

✓ located verbatim in the source · abstract, characters 319–501 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Exposure of calli from mature seeds to 250—500 R gamma rays led to a higher rate of callusing and plantlet regeneration in indica rice.

Organ / stage
calli derived from mature seedse1
Treatment
250—500 Re1
Outcome
rate of callusing and plantlet regeneratione1
Direction
highere1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“somaclonal variation in indica rice”

✓ located verbatim in the source · abstract, characters 128–163 · show in text ↑
e1

“1 kR gamma rays applied to immature embryos and exposure of calli derived from mature seeds at the dose range of 250—500 R led to a higher rate of callusing and plantlet regeneration”

✓ located verbatim in the source · abstract, characters 319–501 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

0.5—1 kR is the appropriate gamma exposure for young inflorescence of indica rice to give a better culture response and higher variability.

Organ / stage
young inflorescencee2
Outcome
culture responsee2
Value
0.5—1 kRe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“somaclonal variation in indica rice”

✓ located verbatim in the source · abstract, characters 128–163 · show in text ↑
e2

“0.5—1 kR would be the appropriate exposure for young inflorescence to produce a better culture response and higher variability”

✓ located verbatim in the source · abstract, characters 503–629 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

2.5—5.0 kR is the optimal gamma dose range for mature embryos of indica rice.

Organ / stage
mature embryose3
Outcome
optimal dose rangee3
Value
2.5—5.0 kRe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“somaclonal variation in indica rice”

✓ located verbatim in the source · abstract, characters 128–163 · show in text ↑
e3

“2.5—5.0 kR the optimal dose range for mature embryos”

✓ located verbatim in the source · abstract, characters 635–687 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Immersion of mature seed in 2—4 mM sodium azide is a concentration level that induces these effects in indica rice.

Organ / stage
mature seede4
Treatment
sodium azidee4
Outcome
concentration level to induce these effectse4
Value
2—4 mMe4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e4

“Immersion of mature seed in 2—4 mM sodium azide solution is a concentration level to induce these effects in indica rice”

✓ located verbatim in the source · abstract, characters 689–809 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Optimal gamma exposure combined with a chemical mutagen enhanced the somaclonal variation frequency in rice explants.

Treatment
optimal gamma exposure and chemical mutagene5
Outcome
somaclonal variation frequencye5
Direction
enhancede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e5

“Application of optimal gamma exposure and chemical mutagen to rice explants enhanced the somaclonal variation frequency”

✓ located verbatim in the source · abstract, characters 811–930 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Young inflorescence and mature embryo of indica rice were satisfactory explants for in vitro culture and mutagenesis.

Organ / stage
young inflorescence and mature embryoe6
Outcome
satisfactory explantse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“somaclonal variation in indica rice”

✓ located verbatim in the source · abstract, characters 128–163 · show in text ↑
e6

“The results showed that young inflorescence and mature embryo were satisfactory explants both for in vitro culture and for mutagenesis”

✓ located verbatim in the source · abstract, characters 183–317 · show in text ↑
Method / resourceobservedoriginal resultpositivebasis n/a

Claim · model-written summary

The mutagenesis and culture procedure provided a large number of rice variants useful for breeding.

Outcome
variants useful for rice breedinge7
Value
a large numbere7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e7

“provided a large number of variants useful for rice breeding”

✓ located verbatim in the source · abstract, characters 935–995 · show in text ↑

radiation · growth · Oryza sativa (rice)Results differ

84 papers: 22 positive, 62 negative or no effect

21 papers in the same direction as this paper · 60 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

80 papers more in this group

See all 84 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.