Abstract
MYB transcription factors play important roles in transcriptional regulation of many secondary metabolites including anthocyanins. We cloned the R2R3-MYB type IbMYB1 complementary DNAs from the purple-fleshed sweet potato (Ipomoea batatas L. cv Sinzami) and investigated the expression patterns of IbMYB1 gene with IbMYB1a and IbMYB1b splice variants in leaf and root tissues of various sweet potato cultivars by reverse transcription-polymerase chain reaction. The transcripts of IbMYB1 were predominantly expressed in the purple-fleshed storage roots and they were also detectable in the leaf tissues accumulating anthocyanin pigments. In addition, transcript levels of IbMYB1 gene were up-regulated by treatment with methyl jasmonate or salicylic acid in leaf and root tissues of cv. White Star. To set up the intragenic vector system in sweet potato, we first evaluated the utilization of the IbMYB1 gene as a visible selectable marker. The IbMYB1a was transiently expressed in tobacco leaves under the control of a constitutive cauliflower mosaic virus 35S promoter, a root-specific and sucrose-inducible sporamin promoter, and an oxidative stress-inducible sweet potato anionic peroxidase2 promoter. We also showed that overexpression of IbMYB1a induced massive anthocyanin pigmentation in tobacco leaves and up-regulated the transcript levels of the structural genes in anthocyanin biosynthetic pathway. Furthermore, high-performance liquid chromatography analysis revealed that the expression of IbMYB1a led to production of cyanidin as a major core molecule of anthocyanidins in tobacco leaves. These results suggest that the IbMYB1 gene can be applicable to a visible marker for sweet potato transformation with intragenic vectors, as well as the production of anthocyanin as important nutritive value in other plant species.
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Claims 13
Claim · model-written summary
IbMYB1 cDNAs with two splice variants were cloned from purple fleshed sweet potato.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatast0e0 - Material
- cv Sinzamie0
- Gene
- IbMYB1e0
- Outcome
- IbMYB1 complementary DNAse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“sweet potatoIbMYB1gene”
e0“We cloned the R2R3-MYB type IbMYB1 complementary DNAs from the purple-fleshed sweet potato (Ipomoea batatas L. cv Sinzami)”
Claim · model-written summary
IbMYB1 transcripts were predominantly expressed in purple fleshed storage roots.
- Taxon
- sweet potatoIpomoea batatase1
- Organ / stage
- purple-fleshed storage rootse0
- Gene
- IbMYB1e0
- Outcome
- transcripts of IbMYB1e0
- Direction
- predominantly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts of IbMYB1 were predominantly expressed in the purple-fleshed storage roots”
e1“sweet potato (Ipomoea batatas L. cv Sinzami)”
Claim · model-written summary
IbMYB1 transcripts were detectable in leaves accumulating anthocyanin.
- Taxon
- sweet potatoIpomoea batatast0
- Organ / stage
- leaf tissuese0
- Outcome
- detectable in the leaf tissues accumulating anthocyanin pigmentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potatoIbMYB1gene”
e0“they were also detectable in the leaf tissues accumulating anthocyanin pigments”
Claim · model-written summary
Methyl jasmonate raised IbMYB1 transcript levels in White Star leaves and roots.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- cv. White Stare0
- Organ / stage
- leaf and root tissuese0
- Treatment
- methyl jasmonatee0
- Gene
- IbMYB1e0
- Outcome
- transcript levels of IbMYB1 genee0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potatoIbMYB1gene”
e0“transcript levels of IbMYB1 gene were up-regulated by treatment with methyl jasmonate or salicylic acid in leaf and root tissues of cv. White Star”
Claim · model-written summary
Salicylic acid raised IbMYB1 transcript levels in White Star leaves and roots.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- cv. White Stare0
- Organ / stage
- leaf and root tissuese0
- Treatment
- salicylic acide0
- Gene
- IbMYB1e0
- Outcome
- transcript levels of IbMYB1 genee0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potatoIbMYB1gene”
e0“transcript levels of IbMYB1 gene were up-regulated by treatment with methyl jasmonate or salicylic acid in leaf and root tissues of cv. White Star”
Claim · model-written summary
IbMYB1a was transiently expressed in tobacco leaves using the constitutive cauliflower mosaic virus 35S promoter.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Treatment
- constitutive cauliflower mosaic virus 35S promotere0
- Gene
- IbMYB1ae0
- Outcome
- transiently expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The IbMYB1a was transiently expressed in tobacco leaves under the control of a constitutive cauliflower mosaic virus 35S promoter”
Claim · model-written summary
IbMYB1a was transiently expressed in tobacco leaves using a root specific and sucrose inducible sporamin promoter.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- root-specific and sucrose-inducible sporamin promotere0
- Gene
- sporamine0
- Outcome
- tobacco leaves under the controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco leaves under the control of a constitutive cauliflower mosaic virus 35S promoter, a root-specific and sucrose-inducible sporamin promoter”
Claim · model-written summary
IbMYB1a was transiently expressed in tobacco leaves using an oxidative stress inducible sweet potato anionic peroxidase2 promoter.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- oxidative stress-inducible sweet potato anionic peroxidase2 promotere0
- Gene
- IbMYB1ae0
- Outcome
- transiently expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The IbMYB1a was transiently expressed in tobacco leaves under the control of a constitutive cauliflower mosaic virus 35S promoter, a root-specific and sucrose-inducible sporamin promoter, and an oxidative stress-inducible sweet potato anionic peroxidase2 promoter.”
Claim · model-written summary
IbMYB1a overexpression induced extensive anthocyanin pigmentation in tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- overexpression of IbMYB1ae0
- Gene
- IbMYB1ae0
- Outcome
- anthocyanin pigmentatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of IbMYB1a induced massive anthocyanin pigmentation in tobacco leaves”
Claim · model-written summary
IbMYB1a overexpression upregulated structural genes in the anthocyanin biosynthetic pathway in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of IbMYB1ae0
- Gene
- structural genes in anthocyanin biosynthetic pathwaye0
- Outcome
- transcript levels of the structural genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of IbMYB1a induced massive anthocyanin pigmentation in tobacco leaves and up-regulated the transcript levels of the structural genes in anthocyanin biosynthetic pathway”
Claim · model-written summary
IbMYB1a expression yielded cyanidin as the major core anthocyanidin molecule in tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Gene
- IbMYB1ae0
- Outcome
- production of cyanidin as a major core molecule of anthocyanidinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of IbMYB1a led to production of cyanidin as a major core molecule of anthocyanidins in tobacco leaves”
Claim · model-written summary
The authors propose IbMYB1 as a visible marker for sweet potato intragenic transformation.
- Taxon
- sweet potatoIpomoea batatast0e0
- Gene
- IbMYB1e0
- Outcome
- visible marker for sweet potato transformation with intragenic vectorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potatoIbMYB1gene”
e0“the IbMYB1 gene can be applicable to a visible marker for sweet potato transformation with intragenic vectors”
Claim · model-written summary
The authors propose IbMYB1 for anthocyanin production in other plant species.
Verbatim
e0“as well as the production of anthocyanin as important nutritive value in other plant species”
The same questions in other papers
salicylic acid · molecular: expression · Ipomoea batatas (sweet potato)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →(-)-methyl jasmonate · molecular: expression · Ipomoea batatas (sweet potato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.