Abstract
Thirty one isolates of endophytic bacteria were collected from different plant sources together with Bacillus subtilis var. amyloliquefaciens (FZB24) that was obtained from Novozymes South Asia Pvt. Ltd. to screen against major pathogens of rice. All the isolates were characterized on the basis of biochemical and phenotypic analysis. Therefore, following these tests, it can be concluded that 31 isolates exhibited differences and they were subjected to partial 16S-rDNA gene sequencing using polymerase chain reaction for phylogenetic analysis. The molecular characterization through amplification of 16S rDNA fragment to an amplicon size of 546 bp confirmed that the thirty one isolates were Bacillus. The PCR analysis showed that all the 31 isolates were found to have the genes for iturin A, 30 isolates for surfactin, 27 isolates for bacillomycin D and one isolate was found to have gene for zwittermicin A. The biosynthetic gene for the production of ACC deaminase was also identified among 11 endophytic Bacillus isolates. The random amplified polymorphic DNA (RAPD) analysis showed more similarity among the isolates isolated from the same ecosystem as compared to the isolates collected from different ecosystems. Among the endophytic bacterial isolates tested against the major pathogens of rice viz., Pyricularia grisea, Rhizoctonia solani and Xanthomonas oryzaepv. oryzae in vitro, Bacillus subtilis var. amyloliquefaciens (FZB24) was found to be effective in inhibiting growth of all the three pathogens and it was also found to promote the growth of rice seedling by registering significantly higher vigour index in roll towel method. Key words: Endophyte, Bacillus, antagonistic activity, antibiotic gene, random amplified polymorphic DNA (RAPD).
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Claims 4
Claim · model-written summary
FZB24 inhibited growth of Pyricularia grisea in vitro.
- Taxon
- rice
Oryza sativat0 - Interactor
- Pyricularia grisea
Pyricularia griseae1pathogen - Second interactor
- Bacillus subtilis var. amyloliquefaciens (FZB24)
Bacillus subtilise0biocontrol_agent - Outcome
- inhibiting growth of all the three pathogense0
- Direction
- inhibitinge0
- Setting
- in vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis var. amyloliquefaciens (FZB24) was found to be effective in inhibiting growth of all the three pathogens”
e1“Pyricularia grisea, Rhizoctonia solani and Xanthomonas oryzaepv. oryzae in vitro”
t0“rice”
Claim · model-written summary
FZB24 inhibited growth of Rhizoctonia solani in vitro.
- Taxon
- riceOryza sativat0
- Interactor
- Rhizoctonia solani
Rhizoctonia solanie1pathogen - Second interactor
- Bacillus subtilis var. amyloliquefaciens (FZB24)Bacillus subtilise0biocontrol_agent
- Outcome
- inhibiting growth of all the three pathogense0
- Direction
- inhibitinge0
- Setting
- in vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis var. amyloliquefaciens (FZB24) was found to be effective in inhibiting growth of all the three pathogens”
e1“Pyricularia grisea, Rhizoctonia solani and Xanthomonas oryzaepv. oryzae in vitro”
t0“rice”
Claim · model-written summary
FZB24 inhibited growth of Xanthomonas oryzaepv. oryzae in vitro.
- Taxon
- riceOryza sativat0
- Interactor
- Xanthomonas oryzaepv. oryzaee1pathogen
- Second interactor
- Bacillus subtilis var. amyloliquefaciens (FZB24)Bacillus subtilise0biocontrol_agent
- Outcome
- inhibiting growth of all the three pathogense0
- Direction
- inhibitinge0
- Setting
- in vitroe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis var. amyloliquefaciens (FZB24) was found to be effective in inhibiting growth of all the three pathogens”
e1“Pyricularia grisea, Rhizoctonia solani and Xanthomonas oryzaepv. oryzae in vitro”
t0“rice”
Claim · model-written summary
FZB24 promoted rice seedling growth with significantly higher vigour index.
- Taxon
- riceOryza sativae0
- Treatment
- Bacillus subtilis var. amyloliquefaciens (FZB24)e0
- Interactor
- Bacillus subtilis var. amyloliquefaciens (FZB24)Bacillus subtilise0biocontrol_agent
- Outcome
- vigour indexe0
- Direction
- highere0
- Setting
- roll towel methode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis var. amyloliquefaciens (FZB24) was found to be effective in inhibiting growth of all the three pathogens and it was also found to promote the growth of rice seedling by registering significantly higher vigour index in roll towel method.”
The same questions in other papers
Bacillus subtilis · growth · Oryza sativa (rice)Results agree
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.