Abstract
Rice blast disease caused by fungus Magnaporthe oryzae (syn. Pyricularia oryzae) is one of the most damaging diseases of rice, reducing plant production worldwide. In the present study, Bacillus subtilis strain GB519 was identified from the rhizosphere based on predicted signatures of 16S ribosomal DNA and gyrA gene and morphological, biochemical, and physiological characteristics. Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus. In culture, GB519 inhibited growth of the following rice fungal pathogens (in order from most effective to least effective): M. oryzae, Ustilaginoidea virens, Fusarium graminearum, F. moniliforme, F. oxysporum, and Rhizoctonia solani. Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively. Such reductions were correlated with accumulated hydrolytic enzymes, including amylases, proteases, chitinase, and lipases, and the defense enzyme activity of the total antioxidant capacity, catalase, and superoxide dismutase in rice. Field experiments showed that the biocontrol efficacy of GB519 was similar to that of other biological and chemical fungicides. Our results indicate that B. subtilis strain GB519 promoted plant growth and reduced blast disease and suggest that this strain has potential to be used as a biological control agent against rice blast. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Claims 14
Claim · model-written summary
GB519 treatment increased rice germination rate, vigor index.
- Taxon
- rice
Oryza sativae0 - Treatment
- B. subtilis GB519e0
- Interactor
- B. subtilis GB519
Bacillus subtilise0biocontrol_agent - Outcome
- germination rate, vigor indexe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus.”
Claim · model-written summary
GB519 treatment increased rice shoot length, shoot fresh weight, and root fresh weight.
- Taxon
- riceOryza sativae0
- Treatment
- B. subtilis GB519e0
- Interactor
- B. subtilis GB519Bacillus subtilise0biocontrol_agent
- Outcome
- shoot length, shoot fresh weight, and root fresh weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus.”
Claim · model-written summary
GB519 treatment increased rice indole acetic acid.
- Taxon
- riceOryza sativae0
- Treatment
- B. subtilis GB519e0
- Interactor
- B. subtilis GB519Bacillus subtilise0biocontrol_agent
- Outcome
- indole acetic acide0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus.”
Claim · model-written summary
GB519 treatment increased rice organic phosphorus.
- Taxon
- riceOryza sativae0
- Treatment
- B. subtilis GB519e0
- Interactor
- B. subtilis GB519Bacillus subtilise0biocontrol_agent
- Outcome
- organic phosphoruse0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus.”
Claim · model-written summary
GB519 treatment increased rice inorganic phosphorus.
- Taxon
- riceOryza sativae0
- Treatment
- B. subtilis GB519e0
- Interactor
- B. subtilis GB519Bacillus subtilise0biocontrol_agent
- Outcome
- inorganic phosphoruse0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treated with B. subtilis GB519, rice plants exhibited increased germination rate, vigor index, shoot length, shoot fresh weight, and root fresh weight coupled with more production of indole acetic acid, organic phosphorus, and inorganic phosphorus.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 70.3% in greenhouse in 2017.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 70.3e0
- Qualifier
- 2017e0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 62.1% in fields in 2017.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 62.1%e0
- Qualifier
- 2017e0
- Setting
- fieldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 69.9% in greenhouse in 2018.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 69.9e0
- Qualifier
- 2018e0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 71.6% in fields in 2018.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 71.6%e0
- Qualifier
- 2018e0
- Setting
- fieldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 75.1% in greenhouse in 2019.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 75.1e0
- Qualifier
- 2019e0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
GB519 spraying significantly reduced rice blast incidence by 75.6% in fields in 2019.
- Taxon
- riceOryza sativae0
- Treatment
- sprayed with GB519e0
- Interactor
- GB519e0biocontrol_agent
- Outcome
- rice blast incidencee0
- Direction
- reductionse0
- Value
- 75.6%e0
- Qualifier
- 2019e0
- Setting
- fieldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three years of studies showed that, when rice was sprayed with GB519, there were significant reductions in rice blast incidence in both the greenhouse and fields: 70.3 and 62.1% in 2017, 69.9 and 71.6% in 2018, and 75.1 and 75.6% in 2019, respectively.”
Claim · model-written summary
Reduced blast incidence correlated with hydrolytic enzymes.
- Taxon
- riceOryza sativae0
- Gene
- amylases, proteases, chitinase, and lipasese0
- Outcome
- reductionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such reductions were correlated with accumulated hydrolytic enzymes, including amylases, proteases, chitinase, and lipases, and the defense enzyme activity of the total antioxidant capacity, catalase, and superoxide dismutase in rice.”
Claim · model-written summary
Reduced blast incidence correlated with defense enzyme activity.
- Taxon
- riceOryza sativae0
- Gene
- catalase, and superoxide dismutasee0
- Outcome
- reductionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such reductions were correlated with accumulated hydrolytic enzymes, including amylases, proteases, chitinase, and lipases, and the defense enzyme activity of the total antioxidant capacity, catalase, and superoxide dismutase in rice.”
Claim · model-written summary
GB519 biocontrol efficacy was similar to other fungicides in field experiments.
- Taxon
- RiceOryza sativat0
- Outcome
- biocontrol efficacy of GB519e0
- Direction
- similare0
- Comparator
- other biological and chemical fungicidese0
- Setting
- Fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field experiments showed that the biocontrol efficacy of GB519 was similar to that of other biological and chemical fungicides.”
t0“Promotes Rice Growth”
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