Abstract
A passive light microscope system has been developed, capable of reconstructing an extended-focus 3-D cell-level image of chlorophyll fluorescence and Phi(PSII) of intact attached leaves using a limited number of focal plane images of chlorophyll fluorescence. Using this system, the relationships between the depth of the mesophyll cells in spongy tissue and the intensity of the chlorophyll fluorescence and the Phi(PSII) were investigated in sunflower leaves exposed to 300 ppb ozone for 12 h at a PPFD of 300 micromol m(-2) s(-1) actinic light. After ozone exposure, fluorescence intensity (F) largely decreased in the cells just under the epidermal cells (within approximately 20 microm of the epidermal cells), but the sites where fluorescence intensity decreased had no relationship to the position of the stomata. By contrast, the distribution of Phi(PSII) showed no change after the ozone exposure. These findings suggest that ozone-induced inhibition occurs in the cells just under the epidermal cells by reducing the light absorption of the chloroplasts, while the operating quantum efficiency of PSII photochemistry is maintained.
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Claims 5
Claim · model-written summary
A passive microscope reconstructed 3-D cell-level chlorophyll fluorescence and Phi(PSII) images of intact leaves.
- Taxon
- sunflower
Helianthus annuust0 - Outcome
- extended-focus 3-D cell-level image of chlorophyll fluorescence and Phi(PSII)e0
- Value
- 3-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A passive light microscope system has been developed, capable of reconstructing an extended-focus 3-D cell-level image of chlorophyll fluorescence and Phi(PSII) of intact attached leaves using a limited number of focal plane images of chlorophyll fluorescence.”
t0“sunflower leaves”
Claim · model-written summary
Ozone exposure largely decreased fluorescence intensity just under the epidermis.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- ozone exposuree0
- Outcome
- fluorescence intensity (F)e0
- Direction
- largely decreasede0
- Value
- approximately 20 microme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After ozone exposure, fluorescence intensity (F) largely decreased in the cells just under the epidermal cells (within approximately 20 microm of the epidermal cells)”
t0“sunflower leaves”
Claim · model-written summary
Sites of decreased fluorescence had no relationship to stomatal position.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- stomatae0
- Outcome
- fluorescence intensitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sites where fluorescence intensity decreased had no relationship to the position of the stomata”
t0“sunflower leaves”
Claim · model-written summary
Phi(PSII) distribution showed no change after ozone exposure.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- ozone exposuree0
- Outcome
- distribution of Phi(PSII)e0
- Direction
- no changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the distribution of Phi(PSII) showed no change after the ozone exposure”
t0“sunflower leaves”
Claim · model-written summary
The authors suggest ozone inhibits cells beneath the epidermis by reducing chloroplast light absorption while PSII quantum efficiency remains maintained.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- ozonee0
- Outcome
- ozone-induced inhibitione0
- Qualifier
- reducing the light absorption of the chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ozone-induced inhibition occurs in the cells just under the epidermal cells by reducing the light absorption of the chloroplasts, while the operating quantum efficiency of PSII photochemistry is maintained”
t0“sunflower leaves”
The same questions in other papers
ozone · physiology: photosynthesis · Helianthus annuus (common sunflower)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.