Paper · 2012 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Improving adventitious shoot regeneration from cultured leaf explants of Petunia hybrida using thidiazuron

Hassan Abu-Qaoud

4
claims
8
verbatim quotes
5
citations
1
research areas
✓
Open access

Abstract

The effect of various concentrations of thidiazuron (TDZ) with or without 2.7 μM of α-naphthalene acetic acid (NAA) on adventitious shoot formation of two Petunia hybridacultivars was studied. Seeds from ‘Daddy Blue’ and ‘Dreams White’ cultivars were germinated in vitro. Expanded leaves from both seedlings and regenerated shoots were taken as explants. Explants were placed on MS media supplied with different concentrations and combinations of the growth regulators. Cultures were incubated under dark conditions for three weeks and then moved under light condition. The ability to form shoots varied depending on the growth regulator combination. The highest shoot percentage (87.5 to 90%) with both explant sources was obtained when TDZ was used at levels between 0.5 and 8 µM in both cultivars. Regeneration was reduced significantly when TDZ was combined with 2.7 μM NAA. A similar trend of average number of shoots produced was observed. Key words: Cytokinins, petunia, regeneration, thidiazuron.

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Claims 4

Associationobservedoriginal resultmeasured

Claim · model-written summary

Shoot formation ability varied with growth regulator combination.

Taxon
Petunia hybridaPetunia ×hybridat0
Treatment
growth regulator combinatione0
Outcome
ability to form shootse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The ability to form shoots varied depending on the growth regulator combination”

✓ located verbatim in the source · abstract, characters 570–649 · show in text ↑
t0

“Petunia hybrida”

✓ located verbatim in the source · title, characters 73–88 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

TDZ at 0.5 to 8 µM gave the highest shoot percentage, 87.5 to 90%, across both explant sources and cultivars.

Taxon
Petunia hybridaPetunia ×hybridat0
Treatment
TDZ was used at levels between 0.5 and 8 µMe0
Outcome
shoot percentagee0
Direction
higheste0
Value
87.5 to 90%e0
Qualifier
both explant sourcese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest shoot percentage (87.5 to 90%) with both explant sources was obtained when TDZ was used at levels between 0.5 and 8 µM in both cultivars”

✓ located verbatim in the source · abstract, characters 651–799 · show in text ↑
t0

“Petunia hybrida”

✓ located verbatim in the source · title, characters 73–88 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Combining TDZ with 2.7 μM NAA significantly reduced regeneration.

Taxon
Petunia hybridaPetunia ×hybridat0
Treatment
TDZ was combined with 2.7 μM NAAe0
Outcome
Regeneratione0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regeneration was reduced significantly when TDZ was combined with 2.7 μM NAA”

✓ located verbatim in the source · abstract, characters 801–877 · show in text ↑
t0

“Petunia hybrida”

✓ located verbatim in the source · title, characters 73–88 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The average number of shoots followed a similar trend of response to growth regulators.

Taxon
Petunia hybridaPetunia ×hybridat0
Material
Petunia hybridat0
Outcome
average number of shoots producede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A similar trend of average number of shoots produced was observed”

✓ located verbatim in the source · abstract, characters 879–944 · show in text ↑
t0

“Petunia hybrida”

✓ located verbatim in the source · title, characters 73–88 · show in text ↑

Thidiazuron · growth · Petunia x hybrida (garden petunia)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

1-naphthaleneacetic acid · growth · Petunia x hybrida (garden petunia)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

11 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.