Abstract
A study was initiated through various experiments aiming to optimize different stages including callus induction, shoot regeneration, shoot multiplication, rooting and acclimatization of Petunia hybrida. Callogenesis from leaves of in vitro-grown seedling was observed on MS medium supplemented with 0.1 mg/l NAA combined with BAP at various concentrations (0.0, 0.25, 0.5 and 1.0 mg/l). The results indicated that the medium with BAP at 0.5 mg/l in combination with NAA at 0.1 mg/l resulted in the highest percentage for explants producing callus (40%). Callus was transferred to regeneration medium to study the effect of three types of cytokinins including BAP, TDZ and Kin at different concentrations (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/l). The results showed that BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%). Another experiment employed four MS basal salt strengths (full, ¾, ½ and ¼ MS) in combination with three concentrations of sucrose (20, 30 and 40 g/l). Full strength MS basal salt medium with sucrose (40 g/l) boosted multiplication performance. Multiplied shoots were studied for rooting ability. Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm). The well rooted plantlets were successfully acclimatized on different potting media including peat moss, vermiculite, clay and perlite where clay surpassed other growing medium mixtures with a 89% survival rate.
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Claims 9
Claim · model-written summary
BAP at 0.5 mg/l with NAA at 0.1 mg/l produced the highest callus-forming explant percentage, 40%.
- Taxon
- Petunia hybrida
Petunia ×hybridat0 - Organ / stage
- explantse0
- Treatment
- BAP at 0.5 mg/l in combination with NAA at 0.1 mg/le0
- Outcome
- percentage for explants producing calluse0
- Direction
- higheste0
- Value
- 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the medium with BAP at 0.5 mg/l in combination with NAA at 0.1 mg/l resulted in the highest percentage for explants producing callus (40%)”
t0“Petunia hybrida”
Claim · model-written summary
BAP at 0.2 mg/l induced the highest multiplication capacity, 90%.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- BAP at 0.2 mg/le0
- Outcome
- multiplication capacitye0
- Direction
- higheste0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%)”
t0“Petunia hybrida”
Claim · model-written summary
TDZ at 0.2 induced the highest multiplication capacity, 90%.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- TDZ at 0.2e0
- Outcome
- multiplication capacitye0
- Direction
- higheste0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%)”
t0“Petunia hybrida”
Claim · model-written summary
0.4 mg/l induced the highest multiplication capacity, 90%.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- 0.4 mg/le0
- Outcome
- multiplication capacitye0
- Direction
- higheste0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%)”
t0“Petunia hybrida”
Claim · model-written summary
Full-strength MS basal salt medium with 40 g/l sucrose boosted multiplication performance.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- Full strength MS basal salt medium with sucrose (40 g/l)e0
- Outcome
- multiplication performancee0
- Direction
- boostede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Full strength MS basal salt medium with sucrose (40 g/l) boosted multiplication performance”
t0“Petunia hybrida”
Claim · model-written summary
Sucrose at 15 g/l with full or half-strength MS produced rooting percent of 100%.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- sucrose at the lowest concentration (15 g/l) with full or ½ MSe0
- Outcome
- rooting percente0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm)”
t0“Petunia hybrida”
Claim · model-written summary
Sucrose at 15 g/l with full or half-strength MS produced number of roots of 8.33.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- sucrose at the lowest concentration (15 g/l) with full or ½ MSe0
- Outcome
- number of rootse0
- Direction
- higheste0
- Value
- 8.33e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm)”
t0“Petunia hybrida”
Claim · model-written summary
Sucrose at 15 g/l with full or half-strength MS produced roots of 12.03 cm.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- sucrose at the lowest concentration (15 g/l) with full or ½ MSe0
- Outcome
- rootse0
- Direction
- longeste0
- Value
- 12.03 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm)”
t0“Petunia hybrida”
Claim · model-written summary
Clay gave the highest acclimatization survival rate, 89%, among tested potting media.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- claye0
- Outcome
- survival ratee0
- Direction
- surpassede0
- Value
- 89%e0
- Comparator
- other growing medium mixturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clay surpassed other growing medium mixtures with a 89% survival rate”
t0“Petunia hybrida”
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