Paper · 1974 · Ikushugaku zasshi

Effect of Cultural Condition on the in vitro Development of Ovules in Petunia hybrida Vilm.

Takumi WAKIZUKA; Tetsuo NAKAJIMA

4
claims
8
verbatim quotes
11
citations
2
research areas
✓
Open access

Abstract

The successful development of the young ovules excised separately from placenta soon aLter fertilization has not been obtained in Petunia sp.. This experiment was carried out to find a favourable condition for the in vitro develop-ment of the excised young ovules of Petunia hybrida Vilm. The results are as follow : (1) The medium containing 6% sucrose was most favourable for development of the free ovules excised 4 days after pollination. And also when osmotic value of the medium were adjusted to equivalent to 6% sucrose solution by adding cucumber fruit-juice, the ovules were developed at same rate of the former. Therefore, it is recognized that high osmotic value of the medium act upon the development of young ovules. (2) When the ovules excised 3 days after pollination were inoculated, the most favourable bsmotic value of a medium was equivalent to 8% sucrose solution which was higher than one L0r culturing the ovules excised 4 days aLter pollination. Moreover, it was observed that the range of osmotic value for culturing younger ovules was narrow than that for older one. Therefore, it is recognized the osmotic value of medium affects more remarkably the development of younger ovules.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Medium with 6% sucrose was most favourable for development of ovules excised four days after pollination.

Taxon
Petunia hybrida Vilm.Petunia ×hybridat0
Organ / stage
free ovulese0
Treatment
medium containing 6% sucrosee0
Outcome
development of the free ovulese0
Direction
most favourablee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The medium containing 6% sucrose was most favourable for development of the free ovules excised 4 days after pollination”

✓ located verbatim in the source · abstract, characters 321–441 · show in text ↑
t0

“Petunia hybrida Vilm.”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cucumber fruit-juice adjusted to an equivalent osmotic value gave the same ovule development rate.

Taxon
Petunia hybrida Vilm.Petunia ×hybridat0
Treatment
adding cucumber fruit-juicee0
Outcome
ovules were developede0
Direction
same ratee0
Comparator
6% sucrose solutione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“when osmotic value of the medium were adjusted to equivalent to 6% sucrose solution by adding cucumber fruit-juice, the ovules were developed at same rate of the former”

✓ located verbatim in the source · abstract, characters 452–620 · show in text ↑
t0

“Petunia hybrida Vilm.”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

For ovules excised three days after pollination, optimum osmotic value was equivalent to 8% sucrose, higher than for four-day ovules.

Taxon
Petunia hybrida Vilm.Petunia ×hybridat0
Organ / stage
ovulese0
Outcome
bsmotic value of a mediume0
Direction
highere0
Value
8% sucrosee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the most favourable bsmotic value of a medium was equivalent to 8% sucrose solution which was higher than one L0r culturing the ovules excised 4 days aLter pollination”

✓ located verbatim in the source · abstract, characters 800–967 · show in text ↑
t0

“Petunia hybrida Vilm.”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Younger ovules had a narrower culturable osmotic range than older ovules.

Taxon
Petunia hybrida Vilm.Petunia ×hybridat0
Organ / stage
younger ovulese0
Outcome
range of osmotic valuee0
Direction
narrowe0
Comparator
older onee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia x hybrida
Petunia ×hybrida · TaxonW. Bulach · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the range of osmotic value for culturing younger ovules was narrow than that for older one”

✓ located verbatim in the source · abstract, characters 1000–1090 · show in text ↑
t0

“Petunia hybrida Vilm.”

✓ located verbatim in the source · title, characters 70–91 · show in text ↑

sucrose · growth · Petunia x hybrida (garden petunia)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

in vitro · growth · Petunia x hybrida (garden petunia)Positive and negative results

10 papers: 8 positive, 2 negative or no effect

7 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.