Abstract
An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.
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Claims 5
Claim · model-written summary
MS medium with 1 mg/l BAP and 1 mg/l NAA gave the highest Petunia shoot length.
- Taxon
- Petunia hybrida
Petunia ×hybridat0 - Treatment
- MS medium supplemented with 1 mg/l BAP + 1 mg/l NAAe0
- Outcome
- shoot lengthe0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA.”
t0“Petunia hybrida”
Claim · model-written summary
MS medium with 0.6 mg/l BAP and 0.5 mg/l IBA gave the highest Petunia shoot number, three per explant.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBAe0
- Outcome
- shoot numbere0
- Direction
- higheste0
- Value
- 3 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA.”
t0“Petunia hybrida”
Claim · model-written summary
MS medium with 0.2 mg/l GA3 and 0.2 mg/l BAP gave the highest Petunia shoot length of 5.75 cm.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAPe0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 5.75 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP.”
t0“Petunia hybrida”
Claim · model-written summary
Regenerated Petunia shoots rooted on MS medium supplemented with IBA and NAA.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- MS medium supplemented with 0.1-1 mg/1 IBA and NAAe0
- Outcome
- Rooting of regenerated shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA.”
t0“Petunia hybrida”
Claim · model-written summary
Regenerated Petunia shoots established in peat moss pots under laboratory conditions with 70% survival.
- Taxon
- Petunia hybridaPetunia ×hybridat0
- Treatment
- pots containing sterilized peat mosse0
- Outcome
- survival ratese0
- Value
- 70%e0
- Setting
- laboratory conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.”
t0“Petunia hybrida”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.