Abstract
An important part of the root system is the root hairs, which play a role in mineral and water uptake. Here, we present an analysis of the transcriptomic response to water deficiency of the wild-type (WT) barley cultivar 'Karat' and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress resulted in the identification of genes whose expression was specifically affected in each genotype. At the onset of water stress, more genes were modulated by water shortage in the roots of the WT plants than in the roots of rhl1.a. The roots of the WT plants, but not of rhl1.a, specifically responded with the induction of genes that are related to the abscisic acid biosynthesis, stomatal closure, and cell wall biogenesis, thus indicating the specific activation of processes that are related to water-stress signalling and protection. On the other hand, the processes involved in the further response to abiotic stimuli, including hydrogen peroxide, heat, and high light intensity, were specifically up-regulated in the leaves of rhl1.a. An extended period of severe stress caused more drastic transcriptome changes in the roots and leaves of the rhl1.a mutant than in those of the WT. These results are in agreement with the much stronger damage to photosystem II in the rhl1.a mutant than in its parent cultivar after 10 d of water stress. Taking into account the putative stress sensing and signalling features of the root hair transcriptome, we discuss the role of root hairs as sensors of environmental conditions.
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Claims 7
Claim · model-written summary
Water stress affected distinct gene sets in wild-type barley and rhl1.a roots.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree2 - Material
- rhl1.ae1
- Outcome
- genes whose expression was specifically affected in each genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“genes whose expression was specifically affected in each genotype”
e2“wild-type (WT) barley cultivar”
e1“Here, we present an analysis of the transcriptomic response to water deficiency of the wild-type (WT) barley cultivar 'Karat' and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress”
Claim · model-written summary
At onset of water stress, more root genes were modulated in WT than rhl1.a.
- Taxon
- barleyHordeum vulgaree2
- Material
- WTe0
- Organ / stage
- rootse0
- Treatment
- water shortagee0
- Outcome
- genes were modulatede0
- Direction
- moree0
- Comparator
- rhl1.ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the onset of water stress, more genes were modulated by water shortage in the roots of the WT plants than in the roots of rhl1.a.”
e2“wild-type (WT) barley cultivar”
Claim · model-written summary
WT roots specifically induced genes related to abscisic acid biosynthesis, stomatal closure, and cell wall biogenesis; rhl1.a roots did not.
- Taxon
- barleyHordeum vulgaree2
- Material
- WTe0
- Organ / stage
- rootse0
- Treatment
- water stresse1
- Outcome
- genes that are related to the abscisic acid biosynthesis, stomatal closure, and cell wall biogenesise0
- Direction
- inductione0
- Comparator
- rhl1.ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The roots of the WT plants, but not of rhl1.a, specifically responded with the induction of genes that are related to the abscisic acid biosynthesis, stomatal closure, and cell wall biogenesis”
e2“wild-type (WT) barley cultivar”
e1“Here, we present an analysis of the transcriptomic response to water deficiency of the wild-type (WT) barley cultivar 'Karat' and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress”
Claim · model-written summary
rhl1.a leaves specifically up-regulated responses to hydrogen peroxide, heat and high light intensity.
- Taxon
- barleyHordeum vulgaree2
- Material
- rhl1.ae0
- Organ / stage
- leavese0
- Outcome
- processes involved in the further response to abiotic stimuli, including hydrogen peroxide, heat, and high light intensitye0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the processes involved in the further response to abiotic stimuli, including hydrogen peroxide, heat, and high light intensity, were specifically up-regulated in the leaves of rhl1.a”
e2“wild-type (WT) barley cultivar”
Claim · model-written summary
Extended severe stress caused more drastic root transcriptome changes in rhl1.a than WT.
- Taxon
- barleyHordeum vulgaree2
- Material
- rhl1.ae0
- Organ / stage
- rootse0
- Treatment
- An extended period of severe stresse0
- Outcome
- transcriptome changese0
- Direction
- more drastice0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An extended period of severe stress caused more drastic transcriptome changes in the roots and leaves of the rhl1.a mutant than in those of the WT.”
e2“wild-type (WT) barley cultivar”
Claim · model-written summary
Extended severe stress caused more drastic leaf transcriptome changes in rhl1.a than WT.
- Taxon
- barleyHordeum vulgaree2
- Material
- rhl1.ae0
- Organ / stage
- leavese0
- Treatment
- An extended period of severe stresse0
- Outcome
- transcriptome changese0
- Direction
- more drastice0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An extended period of severe stress caused more drastic transcriptome changes in the roots and leaves of the rhl1.a mutant than in those of the WT.”
e2“wild-type (WT) barley cultivar”
Claim · model-written summary
After ten days of water stress, photosystem II damage was much stronger in rhl1.a than the parent cultivar.
- Taxon
- barleyHordeum vulgaree2
- Material
- rhl1.ae0
- Treatment
- water stresse0
- Outcome
- damage to photosystem IIe0
- Direction
- much strongere0
- Qualifier
- 10 de0
- Comparator
- parent cultivare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“much stronger damage to photosystem II in the rhl1.a mutant than in its parent cultivar after 10 d of water stress”
e2“wild-type (WT) barley cultivar”