Abstract
This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of two Middle East chickpea cultivars (Balila and Wady). Organogenic ability of various explants (decapitated embryo axes, fragments of embryo axes, fragments of embryo axes with basal part of cotyledons, entire and half of cotyledons) was tested on MS media containing different concentrations and nature of cytokinins (BAP and zeatin) or compound with cytokinin-like activity (TDZ). The highest level of shoot regeneration was achieved in Balila genotype using embryo axes fragments including the basal part of cotyledon on medium with 0.25 mg/L BAP. Higher BAP concentrations than 0.25 mg/L for Balila and 0.5 mg/L for Wady caused a decrease of shoot regeneration. On MS media supplemented with zeatin (1, 3 and 5 mg/L) and thidiazuron (1, 3 and 5 mg/L), the highest level of shoot differentiation was obtained from calli derived from fragments of embryo axes. Organogenic ability of calli from Wady genotype was enhanced by increasing zeatin and TDZ concentrations, up to 5 mg/L. Conversely, a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.
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Claims 13
Claim · model-written summary
Balila embryo axis fragments with the cotyledon base on 0.25 mg/L BAP gave the highest shoot regeneration.
- Taxon
- chickpea
Cicer arietinume1 - Material
- Balilae0
- Organ / stage
- embryo axes fragments including the basal part of cotyledone0
- Treatment
- medium with 0.25 mg/L BAPe0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest level of shoot regeneration was achieved in Balila genotype using embryo axes fragments including the basal part of cotyledon on medium with 0.25 mg/L BAP.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
BAP concentrations above 0.25 mg/L reduced Balila shoot regeneration.
- Taxon
- chickpeaCicer arietinume1
- Material
- Balilae0
- Treatment
- Higher BAP concentrations than 0.25 mg/Le0
- Outcome
- shoot regeneratione0
- Direction
- decreasee0
- Comparator
- 0.25 mg/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher BAP concentrations than 0.25 mg/L for Balila and 0.5 mg/L for Wady caused a decrease of shoot regeneration.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
BAP concentrations above 0.5 mg/L reduced Wady shoot regeneration.
- Taxon
- chickpeaCicer arietinume1
- Material
- Wadye0
- Treatment
- Higher BAP concentrationse0
- Outcome
- shoot regeneratione0
- Direction
- decreasee0
- Comparator
- 0.5 mg/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher BAP concentrations than 0.25 mg/L for Balila and 0.5 mg/L for Wady caused a decrease of shoot regeneration.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
With zeatin, embryo axis fragment calli gave the highest shoot differentiation.
- Taxon
- chickpeaCicer arietinume1
- Organ / stage
- calli derived from fragments of embryo axese0
- Treatment
- zeatin (1, 3 and 5 mg/L)e0
- Outcome
- shoot differentiatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On MS media supplemented with zeatin (1, 3 and 5 mg/L) and thidiazuron (1, 3 and 5 mg/L), the highest level of shoot differentiation was obtained from calli derived from fragments of embryo axes.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
With thidiazuron, embryo axis fragment calli gave the highest shoot differentiation.
- Taxon
- chickpeaCicer arietinume1
- Organ / stage
- calli derived from fragments of embryo axese0
- Treatment
- thidiazuron (1, 3 and 5 mg/L)e0
- Outcome
- shoot differentiatione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On MS media supplemented with zeatin (1, 3 and 5 mg/L) and thidiazuron (1, 3 and 5 mg/L), the highest level of shoot differentiation was obtained from calli derived from fragments of embryo axes.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing zeatin concentration to 5 mg/L enhanced Wady callus organogenic ability.
- Taxon
- chickpeaCicer arietinume1
- Material
- Wadye0
- Treatment
- increasing zeatine0
- Outcome
- Organogenic ability of callie0
- Direction
- enhancede0
- Value
- 5 mg/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Organogenic ability of calli from Wady genotype was enhanced by increasing zeatin and TDZ concentrations, up to 5 mg/L.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing TDZ concentration to 5 mg/L enhanced Wady callus organogenic ability.
- Taxon
- chickpeaCicer arietinume1
- Material
- Wadye0
- Treatment
- TDZ concentrationse0
- Outcome
- Organogenic ability of callie0
- Direction
- enhancede0
- Value
- 5 mg/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Organogenic ability of calli from Wady genotype was enhanced by increasing zeatin and TDZ concentrations, up to 5 mg/L.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing BAP progressively reduced Balila shoot frequency.
- Taxon
- chickpeaCicer arietinume1
- Material
- Balilae0
- Treatment
- BAPe0
- Outcome
- shoot frequencye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing zeatin progressively reduced Balila shoot frequency.
- Taxon
- chickpeaCicer arietinume1
- Material
- Balilae0
- Treatment
- zeatine0
- Outcome
- shoot frequencye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing TDZ progressively reduced Balila shoot frequency.
- Taxon
- chickpeaCicer arietinume1
- Material
- Balilae0
- Treatment
- TDZe0
- Outcome
- shoot frequencye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing BAP reduced root development in both genotypes.
- Taxon
- chickpeaCicer arietinume2
- Treatment
- BAPe0
- Outcome
- root developmente0
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“a progressive decrease of shoot frequency for Balila and root development for both genotypes”
e2“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing zeatin reduced root development in both genotypes.
- Taxon
- chickpeaCicer arietinume2
- Treatment
- zeatine0
- Outcome
- root developmente0
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“a progressive decrease of shoot frequency for Balila and root development for both genotypes”
e2“two Middle East chickpea cultivars (Balila and Wady)”
Claim · model-written summary
Increasing TDZ reduced root development in both genotypes.
- Taxon
- chickpeaCicer arietinume2
- Treatment
- TDZe0
- Outcome
- root developmente0
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.”
e1“a progressive decrease of shoot frequency for Balila and root development for both genotypes”
e2“two Middle East chickpea cultivars (Balila and Wady)”
The same questions in other papers
Thidiazuron · growth · Cicer arietinum (chickpea)Positive and negative results
1 in the opposite direction
- positive
N-benzyladenine · growth · Cicer arietinum (chickpea)Positive and negative results
10 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.