Abstract
A rapid, simple and efficient protocol for in vitro multiple shoot induction and plantlet regeneration was achieved from two different explants of Cicer arietinum cv (ICCCR) (kranthi). The explants viz shoot tip and cotyledonary node were cultured on MS medium fortified with Benzyl Adeno Purine (BAP) (0.5-3.0 mg/L) and Kinetin (Kn) (0.5-3.0 mg/L) for multiple shoot induction. Multiple shoots proliferation was best observed at 2.0mg/L BAP from all the two explants within three weeks of culture. Of the two different explants tested, BAP was found to be more effective than Kinetin for shoot multiplication. The highest number of shoots (12.0 ± 0.3) was achieved on MS medium augmented with 2.0 mg/L BAP. The medium supplemented with (2.0 mg/L) BAP better than all other media concentrations in cotyledonary node explants. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (0.5-1.0mg/L) and Indole Acetic Acid (IAA) (0.5-1.0mg/L) for root induction. Rooting was observed within two weeks of culture. MS medium supplemented with (1.0 mg/L) IBA proved better with seventy percent rooting after 25 days of implantation. Most of the roots were long and healthy. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 76.3%. Plants looked healthy with no visually detectable phenotypic variations.
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Claims 10
Claim · model-written summary
At 2.0 mg/L BAP, both explants showed their best multiple-shoot proliferation within three weeks.
- Taxon
- Cicer Arietinum L.t0
- Organ / stage
- two explantse0
- Treatment
- 2.0mg/L BAPe0
- Outcome
- Multiple shoots proliferatione0
- Direction
- best observede0
Not reportedComparator
Verbatim
e0“Multiple shoots proliferation was best observed at 2.0mg/L BAP from all the two explants within three weeks of culture”
t0“Cicer Arietinum L.”
Claim · model-written summary
BAP produced more shoot multiplication than Kinetin.
Verbatim
e0“Of the two different explants tested, BAP was found to be more effective than Kinetin for shoot multiplication”
t0“Cicer Arietinum L.”
Claim · model-written summary
MS medium with 2.0 mg/L BAP yielded the maximum of 12.0 ± 0.3 shoots.
- Taxon
- Cicer Arietinum L.t0
- Treatment
- 2.0 mg/L BAPe0
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 12.0 ± 0.3e0
Not reportedComparator
Verbatim
e0“The highest number of shoots (12.0 ± 0.3) was achieved on MS medium augmented with 2.0 mg/L BAP”
t0“Cicer Arietinum L.”
Claim · model-written summary
For cotyledonary node explants, 2.0 mg/L BAP medium outperformed other concentrations.
Verbatim
e0“The medium supplemented with (2.0 mg/L) BAP better than all other media concentrations in cotyledonary node explants”
t0“Cicer Arietinum L.”
Claim · model-written summary
Rooting was observed within two weeks of culture.
Not reportedDirection
Verbatim
e0“Rooting was observed within two weeks of culture”
t0“Cicer Arietinum L.”
Claim · model-written summary
MS medium with 1.0 mg/L IBA produced 70% rooting after 25 days.
- Taxon
- Cicer Arietinum L.t0
- Treatment
- (1.0 mg/L) IBAe0
- Outcome
- rootinge0
- Direction
- bettere0
- Value
- seventy percente0
- Qualifier
- after 25 days of implantatione0
Not reportedComparator
Verbatim
e0“MS medium supplemented with (1.0 mg/L) IBA proved better with seventy percent rooting after 25 days of implantation”
t0“Cicer Arietinum L.”
Claim · model-written summary
Most regenerated roots were long and healthy.
Verbatim
e0“Most of the roots were long and healthy”
t0“Cicer Arietinum L.”
Claim · model-written summary
Rooted plantlets were hardened and established in field conditions.
Verbatim
e0“Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions”
t0“Cicer Arietinum L.”
Claim · model-written summary
Plant survival was 76.3%.
Verbatim
e0“The recorded survival rate of the plants was 76.3%”
t0“Cicer Arietinum L.”
Claim · model-written summary
Regenerated plants appeared healthy without visible phenotypic variation.
Verbatim
e0“Plants looked healthy with no visually detectable phenotypic variations”
t0“Cicer Arietinum L.”
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