Abstract
Alfalfa (Medicago sativa L.)cell suspension cultures accumulated highconcentrations ofthepterocarpan phytoalexin medicarpin, reaching amaximumwithin 24hours after exposure toan elicitor preparation fromcell walls ofthephytopathogenic fungus Colletotrichum lindemuthianum. Thiswaspreceded byincreases intheextractable activities oftheisoflavonoid biosynthetic enzymesL-phenylalanine ammonia-lyase, cinnamic acid4-hydroxylase, 4-coumarate coenzyme A-ligase, chalcone synthase, chalconeisomerase, andisoflavone 0-methyltransferase. Pectic polysaccharides wereweakelicitors ofphenylalanine ammonialyaseactivity butdidnotinducemedicarpin accumulation, whereas reduced glutathione wastotally inactive asanelicitor in this system. Thefungal cell wallextract wasaweakelicitor of thelignin biosynthetic enzymes, caffeic acid0-methyltransferase andconiferyl alcohol dehydrogenase, butdidnotinduce appreciable increases intheactivities ofthehydrolytic enzymeschitinaseand1,3-0-D-glucanase. Theresults arediscussed inrelation totheactivation ofisoflavonoid biosynthesis inother legumes andthedevelopment ofthealfalfa cell culture system asamodel forstudying theenzymology andmolecular biology ofplant defenseexpression.
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Claims 7
Claim · model-written summary
Fungal cell wall elicitor induced medicarpin accumulation in alfalfa cultures, reaching a maximum within 24 hours.
- Taxon
- Alfalfa
Medicago sativae0 - Treatment
- elicitor preparation fromcell wallse0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- medicarpine0
- Direction
- accumulatede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa L.)cell suspension cultures accumulated highconcentrations ofthepterocarpan phytoalexin medicarpin, reaching amaximumwithin 24hours after exposure toan elicitor preparation fromcell walls ofthephytopathogenic fungus Colletotrichum lindemuthianum”
Claim · model-written summary
Activities of six isoflavonoid biosynthetic enzymes rose before medicarpin accumulation.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- elicitor preparatione2
- Outcome
- extractable activities oftheisoflavonoid biosynthetic enzymesL-phenylalanine ammonia-lyase, cinnamic acid4-hydroxylase, 4-coumarate coenzyme A-ligasee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thiswaspreceded byincreases intheextractable activities oftheisoflavonoid biosynthetic enzymesL-phenylalanine ammonia-lyase, cinnamic acid4-hydroxylase, 4-coumarate coenzyme A-ligase, chalcone synthase, chalconeisomerase, andisoflavone 0-methyltransferase”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
e2“after exposure toan elicitor preparation fromcell walls ofthephytopathogenic fungus Colletotrichum lindemuthianum”
Claim · model-written summary
Pectic polysaccharides weakly elicited phenylalanine ammonia lyase activity.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- Pectic polysaccharidese0
- Outcome
- phenylalanine ammonialyaseactivitye0
- Direction
- weakelicitorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pectic polysaccharides wereweakelicitors ofphenylalanine ammonialyaseactivity”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
Claim · model-written summary
Pectic polysaccharides did not induce medicarpin accumulation.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- Pectic polysaccharidese0
- Outcome
- medicarpin accumulatione0
- Direction
- didnotinducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pectic polysaccharides wereweakelicitors ofphenylalanine ammonialyaseactivity butdidnotinducemedicarpin accumulation”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
Claim · model-written summary
Reduced glutathione was inactive as an elicitor in alfalfa cultures.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- reduced glutathionee0
- Outcome
- inactive asanelicitore0
- Direction
- inactivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced glutathione wastotally inactive asanelicitor in this system”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
Claim · model-written summary
Fungal wall extract weakly elicited lignin biosynthetic enzyme activities.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- Thefungal cell wallextracte0
- Outcome
- lignin biosynthetic enzymese0
- Direction
- weakelicitore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thefungal cell wallextract wasaweakelicitor of thelignin biosynthetic enzymes, caffeic acid0-methyltransferase andconiferyl alcohol dehydrogenase”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
Claim · model-written summary
Fungal wall extract did not appreciably increase chitinase or glucanase activities.
- Taxon
- AlfalfaMedicago sativae1
- Treatment
- fungal cell wallextracte2
- Outcome
- activities ofthehydrolytic enzymeschitinaseand1,3-0-D-glucanasee0
- Direction
- didnotinducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“butdidnotinduce appreciable increases intheactivities ofthehydrolytic enzymeschitinaseand1,3-0-D-glucanase”
e1“Alfalfa (Medicago sativa L.)cell suspension cultures”
e2“Thefungal cell wallextract wasaweakelicitor”
The same questions in other papers
polysaccharide · molecular: metabolites · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- no effect
resistance inducer · Colletotrichum lindemuthianum · Medicago sativa (alfalfa)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
resistance inducer · physiology: enzyme activity · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.