Abstract
The aim of the present study was to investigate the importance of the preculture conditions on the survival of redwood (Sequoia sempervirens (D. Don.) Endl.) shoot tips cryopreserved using a combined droplet- vitrification procedure. In vitro grown Sequoia plants were used for cryopreservation. Preculture was critical for the survival of shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, sucrose, mannitol and sorbitol), treatment with 0.5 M sucrose for 24 h was the most efficient one. One-step vitrification protocol with PVS2 for 30 minutes was efficient for shoot regeneration following cryopreservation. After rapid thawing and transfer of shoot tips on recovery medium, 67% growth recovery was observed. Plants produced from control and cryopreserved shoot tips were phenotypically similar.
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Claims 5
Claim · model-written summary
Preculture was critical for survival after cryopreservation.
- Taxon
- redwood
Sequoia sempervirenst0 - Organ / stage
- shoot tipse0
- Outcome
- survival of shoot tips after cryopreservatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preculture was critical for the survival of shoot tips after cryopreservation.”
t0“redwood”
Claim · model-written summary
Sucrose preculture was most efficient among tested sugars and sugar alcohols.
- Taxon
- redwoodSequoia sempervirenst0
- Treatment
- 0.5 M sucrose for 24 he0
- Outcome
- most efficient onee0
- Direction
- most efficiente0
- Comparator
- glucose, sucrose, mannitol and sorbitole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the different tested sugars and sugar alcohols (glucose, sucrose, mannitol and sorbitol), treatment with 0.5 M sucrose for 24 h was the most efficient one.”
t0“redwood”
Claim · model-written summary
One step PVS2 vitrification was efficient for shoot regeneration.
- Taxon
- redwoodSequoia sempervirenst0
- Treatment
- PVS2e0
- Outcome
- shoot regeneration following cryopreservatione0
- Value
- 30 minutese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One-step vitrification protocol with PVS2 for 30 minutes was efficient for shoot regeneration following cryopreservation.”
t0“redwood”
Claim · model-written summary
Growth recovery after thawing was 67%.
- Taxon
- redwoodSequoia sempervirenst0
- Organ / stage
- shoot tipse0
- Outcome
- growth recoverye0
- Value
- 67%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After rapid thawing and transfer of shoot tips on recovery medium, 67% growth recovery was observed.”
t0“redwood”
Claim · model-written summary
Control and cryopreserved plants were phenotypically similar.
- Taxon
- redwoodSequoia sempervirenst0
- Outcome
- phenotypically similare0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants produced from control and cryopreserved shoot tips were phenotypically similar.”
t0“redwood”