Abstract
RUZIC, Dj., T. VUJOVIC and R. CEROVIC , 2012. In vitro preservation of autochthonous plum genotypes. Bulg. J. Agric. Sci., 18: 55-62 The paper presents results of the application of ‘Cold storage’ (CS), an in vitro technique for preservation of cultures at +5 o C in total darkness. This simple in vitro preservation method is effectively employed in large plant gene bank repositories for the purpose of preservation of valuable plant germplasm. In order to protect from extinction autochthonous plums Crvena Ranka (Prunus insititia L.), Sitnica (Prunus domestica L.) and Cherry plum (Prunus cerasifera Ehrh.) a protocol for their in vitro preservation has been developed. Upon the establishment of aseptic culture, the studied genotypes were propagated in vitro on Murashige and Skoog (1962) medium of different hormonal composition, depending on genotype. During CS, in vitro shoots were maintained at +5 o C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after respective period, viability of shoots for further propagation was determined as well as multiplication parameters and length of axial and lateral shoots. Three months after maintaining shoots in CS, shoots of Crvena Ranka and Sitnica showed high shoot viability (in excess of 50%), while in Cherry plum viable shoots were etiolated showing symptoms of leaf necrosis (in more than 20%). However, after 6-month preservation under cold conditions, only shoots of Crvena Ranka survived showing necrosis on shoot tips, but they had viable, chlorotic lateral shoots (in excess of 45% of shoots). All three genotypes showed severe signs of necrosis after 9 months of cold storage.
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Claims 5
Claim · model-written summary
Crvena Ranka shoots had over 50% viability after three months cold storage.
- Taxon
- Prunus insititia L.e1
- Material
- Crvena Rankae0
- Organ / stage
- shootse0
- Treatment
- Three months after maintaining shoots in CSe0
- Outcome
- shoot viabilitye0
- Direction
- highe0
- Value
- in excess of 50%e0
Not reportedComparator
Verbatim
e0“Three months after maintaining shoots in CS, shoots of Crvena Ranka and Sitnica showed high shoot viability (in excess of 50%)”
e1“Crvena Ranka (Prunus insititia L.)”
Claim · model-written summary
Sitnica shoots had over 50% viability after three months cold storage.
- Taxon
- Prunus domestica L.
Prunus domesticae1 - Material
- Sitnicae0
- Organ / stage
- shootse0
- Treatment
- Three months after maintaining shoots in CSe0
- Outcome
- shoot viabilitye0
- Direction
- highe0
- Value
- in excess of 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three months after maintaining shoots in CS, shoots of Crvena Ranka and Sitnica showed high shoot viability (in excess of 50%)”
e1“Sitnica (Prunus domestica L.)”
Claim · model-written summary
Cherry plum shoots showed etiolation and leaf necrosis above 20% after three months.
- Taxon
- Prunus cerasifera Ehrh.
Prunus cerasiferae1 - Material
- Cherry plume0
- Organ / stage
- shootse0
- Outcome
- symptoms of leaf necrosise0
- Value
- in more than 20%e0
- Qualifier
- Three months after maintaining shoots in CSe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Cherry plum viable shoots were etiolated showing symptoms of leaf necrosis (in more than 20%)”
e1“Cherry plum (Prunus cerasifera Ehrh.)”
e2“Three months after maintaining shoots in CS”
Claim · model-written summary
After six months only Crvena Ranka shoots survived, with necrotic tips and viable chlorotic lateral shoots above 45%.
Verbatim
e0“after 6-month preservation under cold conditions, only shoots of Crvena Ranka survived showing necrosis on shoot tips, but they had viable, chlorotic lateral shoots (in excess of 45% of shoots)”
e1“Crvena Ranka (Prunus insititia L.)”
Claim · model-written summary
All three genotypes had severe necrosis after nine months cold storage.
Verbatim
e0“All three genotypes showed severe signs of necrosis after 9 months of cold storage”
e1“autochthonous plums”
The same questions in other papers
cold · growth · Prunus domestica (plum)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.