Abstract
Changes in peroxidase activity as well as isozyme peroxidase pattern were analyzed in five cell wall (CW) fractions of barley roots exposed to 1mM CdCl2for 48 and 72 h. Whereas strong inhibition of peroxidase activity was detected in fraction CW I and weak inhibition in fractions CW II, III and IV, strong activation of peroxidase was observed in fraction V after Cd application. Despite the inhibition of enzyme activity in most CW fractions, induction of several isoperoxidases was discovered after separation on PAGE. In fraction CW I inhibition of one cathodic isoperoxidase and activation of two anodic and two cathodic isoperoxidases was detected. Additional two anodic and one cathodic Cd-induced isozyme appeared in CW II and CW III. Strongly activated anodic isoperoxidase was detected in fraction CW V. Our results demonstrate that monitoring the stress modified peroxidase activity in enzyme extracts using guaiacol as a non-specific peroxidase substrate is not sufficient enough. Since some isozymes can be activated and some inhibited, isozyme pattern analysis is recommended.
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Claims 9
Claim · model-written summary
Cadmium strongly inhibited peroxidase activity in cell wall fraction I.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- 1mM CdCl2e0
- Outcome
- peroxidase activitye1
- Direction
- inhibitione1
- Qualifier
- fraction CW Ie1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“strong inhibition of peroxidase activity was detected in fraction CW I”
Claim · model-written summary
Cadmium weakly inhibited peroxidase activity in fractions II through IV.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 1mM CdCl2e0
- Outcome
- peroxidase activitye2
- Direction
- inhibitione1
- Qualifier
- fractions CW II, III and IVe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“weak inhibition in fractions CW II, III and IV”
e2“strong inhibition of peroxidase activity was detected in fraction CW I”
Claim · model-written summary
Cadmium strongly activated peroxidase activity in fraction V.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- Cd applicatione1
- Outcome
- peroxidasee1
- Direction
- activatione1
- Qualifier
- fraction Ve1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“strong activation of peroxidase was observed in fraction V after Cd application.”
Claim · model-written summary
Several isoperoxidases were induced despite inhibition of total enzyme activity in most fractions.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 1mM CdCl2e0
- Outcome
- several isoperoxidasese1
- Direction
- inductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“induction of several isoperoxidases was discovered after separation on PAGE.”
Claim · model-written summary
In fraction I, one cathodic isoperoxidase was inhibited.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 1mM CdCl2e0
- Outcome
- one cathodic isoperoxidasee1
- Direction
- inhibitione1
- Qualifier
- fraction CW Ie1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“In fraction CW I inhibition of one cathodic isoperoxidase and activation of two anodic and two cathodic isoperoxidases was detected.”
Claim · model-written summary
In fraction I, two anodic and two cathodic isoperoxidases were activated.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 1mM CdCl2e0
- Outcome
- two anodic and two cathodic isoperoxidasese1
- Direction
- activatione1
- Qualifier
- fraction CW Ie1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“In fraction CW I inhibition of one cathodic isoperoxidase and activation of two anodic and two cathodic isoperoxidases was detected.”
Claim · model-written summary
Two anodic and one cathodic cadmium-induced isozymes appeared in fractions II and III.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- Cd-inducede1
- Outcome
- two anodic and one cathodic Cd-induced isozymee1
- Qualifier
- CW II and CW IIIe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“Additional two anodic and one cathodic Cd-induced isozyme appeared in CW II and CW III.”
Claim · model-written summary
An anodic isoperoxidase was strongly activated in fraction V.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 1mM CdCl2e0
- Outcome
- anodic isoperoxidasee1
- Direction
- activatede1
- Qualifier
- fraction CW Ve1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“Strongly activated anodic isoperoxidase was detected in fraction CW V.”
Claim · model-written summary
The authors recommend isozyme pattern analysis for stress-modified peroxidase activity.
- Taxon
- barleyHordeum vulgaree0
- Outcome
- isozyme pattern analysise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley roots exposed to 1mM CdCl2for 48 and 72 h.”
e1“isozyme pattern analysis is recommended.”
The same questions in other papers
cadmium atom · nutrient: content · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
13 papers more in this group
See all 17 papers →cadmium atom · physiology: antioxidant enzymes · Hordeum vulgare (barley)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.